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Mitochondrial injury will ultimately lead to the release of cytochrome C into cytoplasm,as reflected by the two-fold increase of cytochrome C protein content in alcohol group.

线粒体损伤最终导致细胞色素C释放入胞浆,表现为模型组肝脏胞浆细胞色素C蛋白含量增加至正常对照组的2倍。

Briefly, under fluorescence microscopy, ingested ROS was shown by yellow fluorescence, which was a mixture of red and green, ROS just bounding to MSCs surface only revealed green.

三、检测分化的色素上皮样细胞的吞噬功能1、荧光标记法检测分化的色素上皮样细胞的吞噬功能参照Margaret等的双重荧光标记法。

The results showed that after treatment of neural stem cells by RA, apoptosis factor cytochrome c was released.

同时应用Western blot检测RA诱导神经干细胞凋亡过程中细胞色素C的变化,结果发现在细胞质中有细胞色素C的释放。

Preliminary standard reference spectra for the unliganded enzyme and for the spectral changes induced by sodium dithionite and succinate were obtained. The wavelength and intensity of the UV signal observed in agreement with results of ox-reduced state of heme b.

其次我们使用不同还原剂succinate、Na2S2SO3分别处理两种纯度的样品,通过分光光度计检测细胞色素b的氧化还原状态,观测到了不同还原剂影响下的细胞色素b的特征光谱。

By employing two kinds of molecular marker of mitochondrial DNA, i. e. Cyt b and CoI gene, the sequences of 15 species representing 6 families from Passeriformes were compared. We analysed their sequences compose and characteristic with the sorft of MEG3.1, and built the phylogenetic trees in PAUP~*4.0. We deeply analysed the feasibility that CoI gene qua the marker to research the phylogenetic relationship of Passeriformes, and afford the academic proof for building the DNA barcoding of the birds.

本文分别以线粒体DNA的细胞色素b和CoI基因作为分子标记,对雀形目6科15种鸟类的细胞色素b基因全序列(1,143bp)和CoI基因部分序列(1,176bp)的组成特点及其系统发育树进行了比较分析,还探讨了CoI基因作为雀形目系统发育研究和构建鸟类物种DNA编码库的分子标记的可行性,旨在为DNA编码库的构建积累基础资料。

Objective To study the expression of tumor necrosis factor-α, intercellular adhesion molecule-1(ICAM-1) and S-100 in the process of pigmentary degeneration, and its significance.

目的 观察视网膜色素变性过程中,视网膜外节层内组织细胞肿瘤坏死因子α、神经组织蛋白S-100、细胞间黏附分子-1(ICAM-1)的表达情况,探讨其在视网膜色素变性过程中的作用及意义。

Mainly using protein - film voltammetric technique, we have achieved direct electrochemical response of lots of proteins such as hemoglobin, myoglobin, cytocrome c, cytocrome b5, horseradish peroxiase, GOD ,Vitreoscillia sp. haemoglobin, etc., at a series of protein membrane modified electrodes, for instance, protein/kieselgubr, protein/polyethylene glycol, protein/polyethyleneimine, protein/phosphatidylcholine, protein/DNA, protein/montmorillonite/polyvinyl alcohol, protein/silver nanoparticle/ cysteamine membrane modified electrode, etc., which are prepared by casting, incorperating or layer by layer self-assembly methods. Electrochemical behavior of these proteins in the films have been studied, and their structural and conformational changes have also been examined with spectrometric techniques.

三年来,在基金委的支持下,我们以蛋白膜伏安法这一新技术作为主要的研究手段,并结合其他生物、化学技术,研究了血红蛋白、肌红蛋白、细胞色素C、细胞色素b5、辣根过氧化物酶、葡萄糖氧化酶以及好氧革兰式阴性菌透明颤菌中的血红素类蛋白等多种蛋白质在以涂布和自组装方法制成的蛋白质/硅藻土膜、蛋白质/聚乙二醇膜、蛋白质/卵磷脂膜、蛋白质/聚乙烯亚胺膜、蛋白质/蒙托石/聚乙烯醇自组装膜以及蛋白质/银胶/半胱胺自组装膜等修饰电极上的直接电化学行为,结合各种光谱技术,对蛋白质在膜中的构象进行表征,探索其电子传递机理。

Objective To observe the pathological changes and investigate the hepatocyte apoptosis relating with CYP2E1 in alcoholic liver diseaseof rat. Method the Model of Alcoholic liver Disease: Rats were randomly divided into model group(n=37)and control group(n=33). The dose of 40%ethanol was administerd at 8g/kg body weight by garage twice daily for 8 weeks in model group, and control group was received isovolume saline by gavage. End of 8th week, the activities of serum ALT and AST were detect by autobiochemistry instrument. The pathological changes of liver was observed under light microscope after HE staining, hepatocyte apoptosis was detected by the TUNEL method.

目的: 研究酒精性肝病细胞凋亡程度与细胞色素P4502E1表达的关系方法:用酒精灌胃法制备大鼠酒精性肝病模型,将70只wistar大鼠随机分为正常对照组(33只)和酒精灌胃组(37只),连续灌胃8周后病理切片观察细胞凋亡程度,干化学法测定ALT、AST的含量变化,用PCR法测定细胞色素P450 2E1的表达变化。

At the 10th day after cultivation, the visual pigment immunoreaction was suffusive in the whole cell membrane and apical process; while at the 30th and 40th day, it gathered at the membrane of apical process or one pole of the cells.

体外培养10 d时,视色素弥漫地分布于整个细胞膜及顶端突起;培养20 d时视色素逐渐向顶端突起或细胞的一端集中;到培养30、40 d时,视色素聚集于顶端突起或细胞的一端,呈现极性分布特征。

Vitrectomy allows us to manage otherwise untreatable ocular pathologies, but the substitution of the vitreous by an artificial intraocular irrigation solution would probably disturb the physiology of the retina and choroids.

中文题名眼内灌注液对视网膜色素上皮细胞凋亡的影响副题名外文题名 The effects of different intraocular irrigating solutions on the apoptosis of RPE cells 论文作者殷春悦导师黎晓新教授学科专业眼科学研究领域\研究方向临床医疗技能训练与研究学位级别博士学位授予单位北京大学学位授予日期2001 论文页码总数94页关键词玻璃体切除术眼科手术细胞凋亡视网膜色素上皮细胞眼内灌注液馆藏号BSLW /2001 /R779.6 /42 随着玻璃体切除术的出现和日益发展,眼内灌注液作为术中使用的玻璃体替代物,直接影响视网膜和脉络膜的正常生理功能。

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