色氨酸

Study on the Crystallization Process of Labeled GL-7-ACA Acylase CA130 Complex 7β-bromoacetyl amino cephalosporanic acid (BA-7-ACA), an analog of glutaryl-7-amino cephalosporanic acid (GL-7-ACA), can inhibit and specifically alkylate GL-7-ACA acylase (CA130) from Pseudomonas sp. 130, forming a carbon-carbon bond between BA-7-ACA and the C-2 on indole ring of Trp-β4 residue of CA130.Here we reported that BA-7-ACA labeled CA130 (BA-C130) could self-catalyze the hydrolysis of BA-7-ACA during crystallization process. The hydrolysis was confirmed to be a reaction analogous to the one of GL-7-ACA by comparative MALDI-TOF (matrix-assisted laser desorption/ionization-time of flight) spectrometry analysis.

二、GL-7-AcA酰化酶CA130标记复合物的结晶过程研究溴乙酰氨基头孢烷酸（7β-bromoacetyl amino cephalosporanic acid，BA-7-ACA）作为戊二酰-7-氨基头孢烷酸（GL-7-ACA）的类似物，不仅能够抑制GL-7-ACA酰化酶CA130的活力，而且能通过在BA-7-ACA和CA130的β亚基第四位色氨酸吲哚环二位碳原子之间形成碳-碳共价键而将CA130特异的烷基化。

The levels of all identified metabolites are notably higher at day 11 and day 14. At day 21, the amounts of Asn and His were decreased, but Val, lactate, Ala, malate, succinate, GABA, choline,β-glucose, fructose, allantoid, Phe and formate were all increased. Enteric bacteria must overcome the acid environments in host organs to colonize the host.

结果表明：根瘤菌处理5d后苹果酸含量显著增加，7d时丙氨酸浓度降低而异亮氨酸、苹果酸、琥珀酸、GABA、胆碱、磷酸胆碱、β-葡萄糖、延胡索酸、组氨酸、苯丙氨酸、色氨酸含量显著增加。11d和14d的差别极为显著，所有已鉴定代谢物的含量都大大增高。

Results Agarose gel electrophoresis proved that the length of PCR product was about 7 000 bp. SDS-PAGE showed that the target protein was highly expressed. Compared with those of control, the anthranilate synthase and tryptophan synthase activities of expressed product increased by 2.7 and 3.2 folds respectively.

结果 凝胶电泳可见代PCR扩增产物大小约为7000bp，SDS-PAGE鉴定目的蛋白分别得到了表达，邻氨基苯甲酸合成酶和色氨酸合成酶的活性分别比对照提高了2.7和3.2倍。

The inner reorganization energy and the exothermicity of the reaction are then determined. Closed shell HF SCF calculations have been carried out for the systems Tryptophy1-Tyrosine and Tyrosyl-Tryptophan for different values of R, and the Koopmans^,theorem is invoked to estimate the energy level splitting A.From the obtained A~m~i~n, the values of electron transfer matrix element V~D~A are determined to be 0.96kJ. mol^-^1 and 0.87kJ.mol^-^1 for Tryptophyl-Tyrosine and Tyrosyl- Tryptophan, respectively.

对色氨酰酪氨酸和酪氨酰色氨酸体系进行闭壳层HF自洽场计算，按Koopmans定理计算体系分子轨道分裂能值A，在R约为0处发现了A的极小值，从而获得色氨酰酪氨酸及酪氨酰色氨酸体系分子内电子转移的电子转移矩阵元V~D~A分别为0.96kJ.mol^-^1和0.87kJ.mol^-^1。

Through a variety of resin filter, adsorption and elution conditions for the optimization, as well as decolorization, crystallization methods of research, this article eventually determine the strong acid cation resin HZ-001 which is at pH 3, 3BV / h, having the largest adsorption to the tryptophan; the fermentation broth were centrifuged to remove sediment,then treated with activated carbon to be decolored and then treated with strong acid cation resin HZ-001. After that,2mol/l amino acid were passed through the HZ-001 ion exchanging column,kept almost constants when the flow rate stayed 6 BV / h. At 60℃,it was condensed into the saturated state and then added Ethanol until its percentage reaching 30%. At 4 ℃ under 100rpm placed in the shaker 20h, the collection of L-tryptophan was dried up in vacuo.

本文通过对种树脂的筛选、吸附和洗脱条件的优化，以及对脱色、结晶方法的研究与摸索，最终确定强酸型阳离子树脂HZ-001在pH=3，3倍床体积流速，温度室温，对色氨酸有最大的吸附作用；将发酵液离心去沉淀并用活性炭脱色后通过HZ-001强酸型阳离子交换树脂柱，再用2mol/L的氨水6倍床体积流速在室温下进行洗脱，收集洗脱液，60℃浓缩为过饱和状态后加入30%无水乙醇，4℃下100rpm摇起晶20h，过滤搜集结晶并真空干燥得白色粉片状L-色氨酸晶体。

In the sensitive and simple method, the p-phenylenediamine dihydrochloride was oxided to diazotized products with the reaction of NaNO2 in H2SO4 medium.

色氨酸与重氮化的对苯二胺二盐酸盐在硫酸介质中生成粉红色化合物，该化合物最大吸光值为522nm；摩尔吸光系数为0.89×104L/mol·cm；偶合产物至少稳定存在1h；色氨酸浓度在0.30～12μg/ml范围内遵循朗伯比尔定律。

The conditions for L-tryptophan determination, such as buffer pH value, buffer concentration and applied voltage were investigated.

应用高效毛细管电泳法对发酵液中L-色氨酸的含量进行了测定，研究了检测波长、缓冲液pH值、缓冲液浓度、分离电压对L-色氨酸测定的影响。

Tryptophane – The main sources of tryptophane are beetroots, carrots, celery, spinach, alfalfa and turnips.

色氨酸-的主要来源色氨酸是beetroots ，胡萝卜，芹菜，菠菜，苜蓿和萝卜。

The results of its fluorescence probe showed that when the guanidine hydrochloride concentration in denaturation solution was about 1.0 mol/L,there existed some stable hydrophobic regions,which could interact with a hydrophobic reagent 8-anilino-1-naphthalene sulfonic acid,in the partially folded intermediate of Bacillus amyloliquefaciensα-amylase;with the denaturation concentration increasing,the stable hydrophobic regions disappered.the results of fluorescence quenching using acrylamide and potassium iodide as quenchers showed that using acrylamide as quenchers,with the protein denaturation extent increasing,the number of Trp that can be quenched increased untill all the Trp residues were quenched;Using potassium iodide as quenchers,with the maximum number(8) of tryptophan residues in a partially folded intermediate Bacillus amyloliquefaciensα-amylase molecule could be quenched by potassium iodide;with the denaturation concentration increasing,the number of Trp that can be quenched decreased to 5.the results of their protein electrophoreses and SEC showed that no aggregate or aggregate precipitation of Bacillus amyloliquefaciensα-amylase formed during the whole unfolding/refolding procedure of Bacillus amyloliquefaciensα-amylase induced by guanidine hydrochloride or urea.

ANS外源荧光探针结果表明：盐酸胍诱导的芽孢杆菌α-淀粉酶分子去折叠过程中存在着能够与探针分子1-苯胺基-8-萘磺酸结合的稳定的疏水区域；而随着芽孢杆菌α-淀粉酶分子在盐酸胍溶液中变性程度的加深，这一疏水区域逐步被瓦解。丙烯酰胺和碘化钾猝灭结果表明：在盐酸胍溶液中，随着芽孢杆菌α-淀粉酶分子变性程度的进一步加深，其分子内能够被丙烯酰胺接近的色氨酸残基逐渐增多，直至全部被猝灭。但位于芽孢杆菌α-淀粉酶分子表面的能够被碘化钾猝灭的色氨酸残基，在中间态芽孢杆菌α-淀粉酶分子中数目达到最大的8个，而随着其分子变性程度的进一步加深，反而减少至5个。

Trp and lac operons were usedsas the research object in this paper and two extended mathematical models were proposed on the basis of previous works. The parameters of the model were determined and the results were analyzed. A steady-state optimization model of tryptophan biosynthesis was established based on indirect optimization method. The network analysis was used to lac operon system.

本文以色氨酸操纵子和乳糖操纵子为主要研究对象，在前人的研究基础上建立了较为全面的新的数学模型，确定了模型参数，并对计算结果进行了分析，建立了稳态的优化模型，并利用间接优化法对色氨酸生产过程进行优化；利用网络分析法对乳糖操纵子系统进行分析。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。