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色氨酸

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The effect of chemical modification of amino acid residues upon hemagglutination was investigated.

NBS修饰Trp结果表明,在天然状态下有3个色氨酸分子被修饰,其中第二和第三个色氨酸分子对其活性至关重要。

Visual inspection of spectra shows that the differences observed in spectra of different cells in band intensities at 1603cm^(-1)(C=C in-plane bending mode of phenylalanine and tyrosine) and 1616cm^(-1)(C=C stretching mode of tyrosine and tryptophan) are obvious. This can be used as the marks for blood cell of defferent species. And LTRS is a useful tool for the blood cell analysis.

结果显示不同物种血红细胞的拉曼光谱在1603cm^(-1)苯丙氨酸和色氨酸的C=C平行振动模及1616cm^(-1)酪氨酸和色氨酸的C=C拉伸模有明显的差异,这可以用来作为不同种血红细胞鉴定的特征标记,表明激光镊子拉曼光谱技术可以成为血红细胞分析的有效手段。

Escherichia coli 4-fluorotryptophan-substituted arginyl-tRNA synthetase was biosynthetically prepared and purified from a tryptophan auxotroph, which could overproduce this enzyme.

利用色氨酸营养缺陷型菌株在大肠杆菌精氨酰tRNA合成酶中成功地掺入了4-氟-色氨酸

It was confirmed that more than 95% of the tryptophan residues in the purified 4-fluorotryptophan-substituted arginyl-tRNA synthetase was replaced by 4-fluorotryptophan.

用这种方法分析制备的掺氟酶,证实其中95%以上的色氨酸残基已被4-氟-色氨酸取代。

The effect of N-bromosuccinimide on the activity of Escherichia coli arginyltRNA synthetase was studied.

用N-溴代丁二酰亚胺对大肠杆菌精氨酰tRNA合成酶中的五个色氨酸残基进行化学修饰研究,发现酶分子所含的五个色氨酸残基中有一个可以被。。。

Due to tryptophan's resistance to insulin, this will result in an increase of circulating tryptophan.

由于色氨酸对胰岛素的耐受,结果血液循环中的色氨酸升高。

This will raise blood levels of tryptophan, a chemical that eventually turns into serotonin.

这可以提高血液中的色氨酸水平。色氨酸最终会转化成血清素。

A new synthesis method for 15NL-tryptophan by yeast fermentation using glucose as carbon source, ammonium sulphate as nitrogen source, and 15N anthranilic acid as a precursor is described.

以葡萄糖为碳源,硫酸铵为氮源,15N邻氨基苯甲酸为前体物,利用酵母发酵合成15NL-色氨酸,是合成15NL-色氨酸的一种新方法。

The tryptophan residues play important roles in DNA photoreactivation catalyzed by CPD photolyases. We have investigated the action mechanism concerning Trp in DNA photoreactivation, and obtained the results as follows: 1 We proposed a mechanism of photosensitized splitting of CPDs by Trp and Trp-containing peptides. 2 Quantum yields of splitting of the model compounds that a Trp linked covalently a CPD were obtained under 290 nm light in various solvents, these values changing with solvent, range from 0.01 to 0.5. 3 Utilizing pulse radiolysis technique, splitting processes were observed in aqueous solution of the CPDs with hydrated electron and some oxidative species (such as hydroxyl radical, bromic radical and azide radical), and obtained their rate constants. In the case of interaction between CPD and hydrated electron, splitting of the CPD anion, and pyrimidine anion transfer an electron to FAD or Rf, were observed. The process rate was slower one order more than that in photolyase. Minor, but highly mutagenic UV-light-induced lesions are (6-4) photoproducts, which are repaired by (6-4) photolyases. These enzymes, discovered in 1993, are not well characterized and their mechanism is still under investigation.

我们研究它的作用机理,获得以下结果:1)建立了色氨酸及其肽对嘧啶二聚体光敏化裂解的直接修复作用机理模型;2)获得了色氨酸共价键连接于嘧啶二聚体的化合物在多种溶剂中290nm光辐照裂解的量子效率,其值随溶剂而变化,范围在0.01-0.5;3)运用脉冲辐解观察到嘧啶二聚体与水合电子和一些氧化性的自由基作用裂解过程,得到相应速率常数;对二聚体与水合电子作用发生裂解以及向FAD返还电子的过程进行了体外模拟;4)(6-4)光解酶的作用机理中一个关键假定是氧杂环丁烷中间体在接受电子后能自发裂解,恢复二嘧啶的结构。

The research results indicated that the pretreatment of corn stalk by steam blasting procedure or warm oxygen method could successfully separate lignose and fibre, and enzyme hydrolysis could realize 85 % cellulose conversion rate, then yeast used for fermentation to produce alcohol.

将测定谷物色氨酸的&酶水解三氯化铁醋酸比色法&用于山羊奶、牛奶色氨酸测定,回收率可达96.4%以上。试验结果表明,该法精密度与DAB比色法无显著性差异,但比DAB比色法更快,更简便可靠

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