色氨酸

The influence of pH levels on protein structure is analyzed using fluorescence anisotropism and a new principle is put forward.

首次以不同修饰的金纳米通道阵列为分离载体，分离了色氨酸／VB2，在半胱氨酸、异硫氰酸胍修饰的金纳米通道阵列内，色氨酸／核黄素的相对选择分离系数度分别为5.7和8.6，绝对分离度分别为111.5和168.0。

Recombinant plasmid pFf-22-Trp operon was successfully constructed, and both the anthranilate synthase and tryptophan synthase activities of expressed product increased. It laid a foundation of construction of recombinant E. coli strain for production of tryptophan in a large scale.

已成功构建了重组表达质粒pET-22b-Trp operon，邻氨基苯甲酸合成酶和色氨酸合成酶的活性在大肠杆菌中得到了提高，为高产色氨酸基因工程菌的构建奠定了基础。

A salt of bromic acid .A salt of iodic acid .

5-氟色氨酸(5-FT)、色氨酸氧肟酸盐、6-氟色氨酸(6-FT)、。。。

A conclusion can be drawn that tryptopanase expressed only by constructed plasmid during "catabolite repression".

在葡萄糖存在的条件下由于分解代谢产物阻遏，基因组上的色氨酸酶基因不表达，加入IPTG只表达质粒上的基因，如果在合适条件下测得色氨酸酶活性，则可以证明质粒上的基因能表达出有活性的色氨酸酶。

Because the recovery of 5-hydroxytryptophan from plant is low, and the technics is complex, the plant source is limited. Electroanalysis synthesize method has been used to transform tryptophan to 5-hydroxytryptophan.

由于5-羟基色氨酸从植物中的提取收率较低，工艺复杂，植物来源有限，通过查阅文献资料，采用电解合成法在催化剂存在下，使色氨酸部分转化成为5-羟基色氨酸。

Development period of the parasitoid pupa from the 5th instar larva fed on MSB was significantly longer. Length of cocoon of MSB treatment was significantly shorter than the control. However, no obvious differences were detected in the development periods of egg to larva, cocoon mass size, wasp emergence rate, sex ratio, longevity and forewing length of wasp between KMD1 and the control. 3. Amino acid content in the haemolymph of SSB after fed with transgenic rice Although there were no changes on the components of amino acids in the haemolymph of SSB after fed with two transgenic rice lines, there were some changes on relative contents of amino acids.

取食KMD1 36h后0天，20种氨基酸中除天门冬酰胺、丝氨酸、苏氨酸和甲硫氨酸外，其它16种氨基酸的含量都高于对照，其中组氨酸、甘氨酸、苯丙氨酸、异亮氨酸、赖氨酸差异达显著水平，谷氨酰胺、脯氨酸差异达极显著水平，与氨基酸总量表现一致的变化趋势；取食KMD1后1天，除天门冬酰胺、谷氨酰胺和赖氨酸高于对照外，其它17种氨基酸均较对照低，但仅丝氨酸、苏氨酸和色氨酸与对照差异显著；取食KMD1后3天，绝大多数氨基酸含量的变化与取食KMD1后0天的趋势一致，除丙氨酸和色氨酸比对照稍低，其它18种氨基酸均高于对照，其中精氨酸、缬氨酸差异显著，丝氨酸差异达极显著。

The robustness of this model is discussed by using the S-system model. And the influences of cell growth rate on the biosynthesis of tryptophan, stability and dynamic behavior of the trp operon are investigated. A steady-state optimization model is established. It maximizes the accumulative term accounting for both consumption and secretion. The nonlinear optimization problem is approximately transformed to a linear optimization problem.

本文对表达阻遏蛋白的基因和表达合成色氨酸关键酶的基因之间相互作用进行了研究，通过模型考察了比生长速率对色氨酸生物合成和系统稳定性的影响，对色氨酸合成过程所涉及到的mRNA、酶等的动态行为进行了理论预测和分析，通过S-系统方程将非线性系统转化为线性系统，研究了系统的鲁棒性，并对色氨酸生产进行了优化。

The first part of this paper is to set up a novel mathematical model describing expression and regulation of trp operon, in which not only the effects of repression and attenuation on transcription are taken into consideration, but the interaction between repression gene and structural gene as well as the transportation of tryptophan across cell membrane are also considered.

论文第一部分主要在已有的色氨酸操纵子模型基础上，建立了一个新的用于描述色氨酸操纵子动态行为的数学模型，它不仅考虑了阻遏、弱化以及反馈抑制作用对转录的影响，而且考虑了表达阻遏蛋白的基因和表达关键酶的基因之间的相互作用和影响，以及色氨酸外排的影响。

The thesis is concerned with the fermentation of yeast Candida arborea AS2.566, separation and purification of L-tryptophan and synthesis of 15N labeled anthranilic acid.

本文以发酵法生产L-色氨酸为背景，重点研究了由假丝酵母AS2.566获得L-色氨酸的发酵条件、色氨酸的分离纯化工艺以及邻氨基苯甲酸的合成过程。

The cancers studied here were throat cancer, aute non- lymphoblas He leukem ia and acut lymphoblastic leukemia. The result s indicated that,(1)the levels of Leu, Lys, Ser Asn,Ile,Gly,and Thr were obviously decreased whea lse the levels of orn, Trp evidently increased in cultural liquid of throat can cer cell line indicating that the growth of HepⅡ cell consumed 7 amino acids and releaced 2 amino acids into cultural liquid.(2)the levels of Glu, Gls, Leu, Phe, Tyr and Trp were evidently increased while the levels of Thr , His, Ala decreased in plasma from patient with ANLL. These r esults were consistent with the previous report.(3)the levels of Gly, Trp and Phe in plasma from patients with ANLL evidently de creased and the levels of Ala, His increased after therapy indicating that cance r cells were growing in an anaerobic catabolism state.

这些癌肿包括喉癌HepⅡ细胞，急性非淋巴细胞白血病和急性淋巴细胞白血病，结果表明：(1)喉癌细胞株培养过程中亮氨酸、赖氨酸、丝氨酸、天冬酰胺、异亮氨酸、甘氨酸以及苏氨酸等水平明显降低，而色氨酸水平明显增加，说明喉癌细胞的生长繁殖必须依赖以上7种氨基酸同时释放了色氨酸；(2)急性非淋巴细胞白血病患者血浆中的谷氨酸、甘氨酸、亮氨酸、苯丙氨酸、酪氨酸和色氨酸等水平明显升高，而苏氨酸、组氨酸、丙氨酸等水平明显降低，这些结果与国际报道相一致；(3)经治疗后，ANLL患者血浆中甘氨酸、色氨酸和苯丙氨酸等水平明显降低，而丙氨酸、组氨酸等水平明显升高，表明肿瘤细胞处在无氧代谢。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。