色析法的

First of all, this study will be multi-walled carbon nanotubes by chemical oxidation process purified so it can be modified to deal with than the purification of purity before the modified 5 wt%, more than 1.89 times the surface area to increase the surface potential decrease of about 10 ~ 20 mV and the surface functional base (-COOH and-OH) to increase 1.45 times, and then the control technology of electroless preparation parameters include: analysis of plating time, plating analysis of temperature, metal ions in solution than the (Fe2+/ Ni2+), bath pH and the added value of dispersion Agent and other research towards Fe-Ni particles to increase the iron content and Fe-Ni particles spread in the MWCNT upper fixed targets,the results found that when the parameters for the preparation of 50 ℃, pH10, do not add dispersant, metal ions than the solution (Fe2 +/ Ni2 +) 7, the highest iron content can be Fe = 40.55 at%(flat iron content 111.17 mg / g) of nanocomposites Fe-Ni/CNT, then for a series of the nature of its analysis, the final evaluation Fe-Ni/CNT nanocomposites were processed on the effectiveness of the application of mixed pollutants, the results showed that in 120 minutes at the same time when Adsorption of heavy metal ions lead nitrate 10 ppm, selenium heavy metal ion degradation of 1 ppm and orange azo dye AO7 50 ppm standard of effluents, and after a total Fe-Ni nano-particles to SEM / EDS analysis of more than 50% still remaining.

本研究首先将多壁奈米碳管以化学氧化法做纯化改质处理能使其纯度较纯化改质前提高5 wt％、比表面积提高1.89倍、表面电位下降约10~20 mV以及表面官能基提高1.45倍，再控制无电镀技术之制备参数包括：析镀时间、析镀温度，镀液中金属离子比(Fe2+/Ni2+)、镀液pH值及是否添加分散剂等，研究朝提高Fe-Ni粒子的含铁量与Fe-Ni粒子散布固定在MWCNT上等目标进行，研究结果发现当制备参数为50 ℃、pH10、不添加分散剂、镀液金属离子比(Fe2+/Ni2+)7时，可得到最高铁含量Fe = 40.55 at％(单位铁含量111.17 mg/g)之奈米复合材料Fe-Ni/CNT，接著对其作一系列性质分析，最后评估奈米复合材料Fe-Ni/CNT对共处理混合污染物之应用效益，结果显示其在120分钟时能够同时吸附重金属硝酸铅离子10 ppm、重金属硒酸根离子1 ppm及降解偶氮橘色染料AO7 50 ppm达放流水标准，且共处理后奈米Fe-Ni粒子以SEM/EDS分析仍剩余50 %以上。

The optimum condition of the phase separation colour reaction of gallium with o-chlorophenylfluorone and TritonX-100 has been studied.

研究了镓－邻氯苯基萤光酮－TritonX－100析相显色体系，建立了测定镓的析相光度法。

Methods The antimicrobial peptide of M. domestica pupa were produced largely by infection and injury, which were isolated and purified by trituration, centrifugalization and column chromatography of AKTA purifier. Then the antimicrobial peptides with anti-Toxoplasma activity were selected by haemacytometry and MTT colorimetric method.

通过损伤加感染的方法诱导家蝇蛹大量表达抗菌肽，然后经过研磨、离心和AKTA purifier层析等步骤，将家蝇蛹抗菌肽进行分离纯化，采用血细胞计数法和MTT比色法筛选对弓形虫速殖子有抑制作用的抗菌肽。

objective to isolate and purify the antimicrobial peptides with anti-toxoplasma gondii activity isolated from the haemlymph of musca domestica larvae.methods the antimicrobial peptides of musca domestica larvae were induced by infection and injury were isolated and purified by trituration,centrifugalization and column chromatography.then the antimicrobial peptides with anti-toxoplasma activity were sieved by mtt colorimetric method and haemacytometry.results it was found that two antimicrobial peptides had anti-toxoplasma activity through resource s cationic column chromatography and superdex g75 gel column chromatography.conclusion the two antimicrobial peptides with anti-toxoplasma activity existing in the haemlymph of musca domestica larvae had different anti-toxoplasma effect.

作者单位：山西医科大学寄生虫教研室，太原 030001；化学生物学与分子工程教育部重点实验室山西大学生物技术研究所目的从家蝇幼虫血淋巴中分离纯化具有抗弓形虫作用的抗菌肽。方法通过损伤加感染的方法诱导家蝇幼虫大量表达抗菌肽，然后经过研磨、离心和层析等过程，将家蝇幼虫抗菌肽进行分离纯化，采用四甲基偶氮噻唑蓝比色法和血细胞计数法筛选对弓形虫速殖子有抑制作用的抗菌肽。结果经resource s阳离子柱和superdex g75凝胶柱层析后，筛选出2种对弓形虫速殖子有杀伤作用的抗菌肽。结论家蝇幼虫血淋巴中存在抗弓形虫作用的抗菌肽，而且不止一种，但其效果有所不同。

The object of this study is to evaluate the immunoregulation and anti-tumor activities of Arnebia root polysaccharide in vitro. The ARPS was obtained by hot-water extraction, alcohol precipitation, de-protein, de-color, and dialysis etc. from Arnebia root and was administered on mouse spleen lymphocyte and tumor cell with 24 μgmL^(-1), 121 μgmL^(-1), 364 μgmL^(-1), respectively.

为研究紫草多糖在体外对小鼠脾淋巴细胞的免疫调节活性及对肿瘤细胞的细胞毒作用，本实验采用水提醇沉法，经脱蛋白、脱色、透析等方法提取紫草多糖，MTT法检测紫草多糖对淋巴细胞增殖及对HepG2、SPC-A-1肿瘤细胞的杀伤作用。

First, removing protein by Sevage method. According to literature, it had best efficiency when polysaccharide liquid 2mg·mL~(-1) was treated with chloroform: n-butanol (3:1) and repeated for 3 times. Second, moving pigment with H_2O_2. In order to shorten the time and prevent the broken of structure of polysaccharide, the experiment was operated at 37℃. The following were dialyzation, concentration(60℃) and freeze-drying.

首先Sevage法除蛋白，根据文献，确定氯仿：正丁醇为3：1，多糖浓度2mg·mL~(-1)时，重复三次，除蛋白效果最佳；脱色采用了双氧水氧化法，为加快脱色时间，又要避免双氧水对多糖结构的破坏，实验在37℃下进行；透析、浓缩、干燥过程同样为了保证多糖结构的完整性和提取后物质疏松易溶解，先透析，然后60℃浓缩，最后是冷冻干燥。

Gingerols in ginger oleoresin were separated by AB8 macroporous adsorption resin and silica gel column chromatography. The samples were characterized by the gas chromatography-mass spectrometry method.The peak area normalization method was used to determine the relative percentage of the components and the rate of accumulation of gingerol was determined by contrasting with gingerol standard samples. The results showed that gingerols was concentrated on the 3rd and the 4th parts.

采用硅胶柱层析和AB8大孔树脂吸脱附两种方法分离姜油树脂中的姜酚，采用气相色谱-质谱(gas chromatography-mass spectrometry， GC-MS)对分离样品进行表征，用峰面积归一化法对测试样品的气相色谱-质谱结果进行分析，确定了分离样品中各组分的相对峰面积百分比。

By this method, the productivity of 3. 18g/l extracellular polysaccharides was obtained. The optimum extraction method was obtained by RSA. It was determined as follows: 91. 4 ℃, 2. 9h, the weight ratio between mycelia and water was 1 to 3. The productivity was 12. 38% of dry mycelia. Set the purifying methods of Grifola frondosa: Precipitated polysaccharides part by 60% ethanol→removed protein by Sevag method→removed coloring matter by H〓O〓→removed salts by dialyse→DEAE-cellulose column chromatogram→ purified polysaccharides groups.

对于胞内多糖的提取，采用湿菌体经捣碎再高压破壁的方式破碎菌体细胞，采用响应面分析法得出优化后的提取条件为：提取温度91.4℃，提取时间2.9h，料水比1：3，此提取条件下胞内粗多糖的率为干菌体重的12.38％建立了灰树花多糖分离纯化的技术路线 60％乙醇沉淀的粗多糖→Sevag法脱蛋白→H〓O〓脱色→透析脱盐→DEAE-纤维素层析分离→多糖组分。

The results showed: when TLC solvent system was: chloroform - methanol - diethyl amine = 90:9:1, alkaloids from lotus leaves were well isolated, and five components were isolated and identified by spraying Dragendorff reagent. The optimal conditions of HPLC solvent system were: methanol- water - diethyl amine = 75:25:0.0125. Under the conditions, components of alkaloids from lotus leaves were separated very well, and ideal RP-HPLC peaks were obtained.5、Methods of High Speed Counter Current Chromatography and preparative High Performance Liquid Chromatography applied to identify and purify alkaloids from lotus leaves were set up. When solvent system of HSCCC was: chloroform - methanol - water (pH=4.00)= 4:3:2, speed of chromatogram was 700rpm, flow speed of the mobile phase was 2mL/min, four pure components relatively were attained by isolation and purification.

结果表明：薄层层析溶剂系统为氯仿：甲醇：二乙胺=90：9：1，使荷叶中的生物碱达到了较好的分离效果，并用改良的碘化铋钾试剂喷雾显色，共分离鉴别出了5种荷叶生物碱；分析型HPLC分析检测的较优溶剂系统为甲醇：水：二乙胺=75：25：0.0125，使荷叶生物碱的各个组分达到了基线分离，并获得了较好的峰形。5、建立了高速逆流色谱和制备型HPLC技术分离纯化荷叶生物碱的具体方法。H(来源：ABC46论文网www.abclunwen.com)SCCC法在溶剂系统为氯仿：甲醇：pH4.00的水=4：3：2，色谱仪转速为700rpm，流动相流速为2mL／min的条件下，分离纯化得到了4个纯度较高的化合物。

METHOD:Porcine hemoglobin with high purity and activity was prepared by centrifuging,micro filtration and weak anion exchange chromatography.

方法通过离心、微滤和弱阴离子交换层析法，制备高纯度高活性的猪血红蛋白，并建立合理的检验方法(紫外可见光扫描、电泳、高效液相色谱、血凝试验抑制试验和血气分析等)，以确定纯化产品的含量、纯度和活性。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。