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The main collagens in skin are type I and III. In this research, total collagens were separated from rat skin by enzymatic digest and acetum methods. The collagens were denatured at 60℃ and digested with trypsin. Characteristic peptides typical for collagen type I and III were identified with high performance liquid chromatography/mass spectrometry.

哺乳动物皮肤真皮中胶原蛋白含量约为70%,主要为是I型、III型胶原蛋白,本实验利用稀酸溶解和酶法提取了大鼠皮肤中的总胶原蛋白,将胶原蛋白粗提品在60℃变性后用胰蛋白酶进行降解,液相色谱/质谱联用法分析了两种胶原蛋白的特征多肽,利用特征多肽比较了不同生长期大鼠皮肤中I型和III型胶原蛋白相对含量。

Guangdong college of pharmacy,guangzhou,guangdong 510006,china;henan university of traditional chinese medicine,zhengzhou,henan 450008,chinaabstract:objective to establish a method for the determination of the content of total alkaloid in nao mai tong recipe.methods acid dye colorimetry was used in the determination with bromocresol green buffer solution as the color developing agent with absorbance was determined at the detection wavelength of 417 nm.results the calibration curve of the absorbance was linear with the concentration of ligustrazine in the range of 0.031~0.153 mg/ml(r=0.9998).the average recovery of ligustrazine was 100.95%,rsd=1.75%.

作者单位:广东药学院中药化学与分析教研室,广东广州 510006;河南中医学院,河南郑州 450008 目的建立脑脉通有效部位中总生物碱的含量测定方法。方法采用酸性染料比色法,以溴甲酚绿缓冲液作为生物碱的显色剂,测定样品溶液在417 nm波长处的吸收度。结果对照品川芎嗪质量浓度在0.031~0.153 mg/ml的范围内与吸光度呈良好的线性关系,r=0.9998。平均回收率为100.95%,rsd为1.75%。精密度和稳定性良好。

Just as combining different proportions of the additive primaries allows millions of different colors to be produced, the overprinting of different amounts of cyan, magenta, and yellow also creates a large gamut of different colors.

就像加色法基色通过不同比例混合能产生数百万不同的颜色一样,通过不同数量的青色、品红、黄色的混合印刷也可以产生宽广的色谱。

If the absorbance of the three process colors were correctly matched to the spectral response of the eye,printing cyan,magenta,and yellow on top of each other would absorb all of the additive primariesto produce black.In practice,overprinting these three colors creates a dark brown,so to enable a wider range of densities to be printed a black ink must also be used.The black also enhances the edges of an image and reduces the amount of expensive colored inks that need to be used.The four colors cyan,magenta,yellow,and black are often abbreviated as CMYK,with the last letter of the word black used instead of the first to avoid confusion with blue.Many people in the praphic arts industry refer to cyan and magenta as"process blue"and"process red"(or,confusingly,as just blue and red).As well as any pigment that may be present,substrates have other properties that affect color appearance.

如果吸收的三色过程中,正确匹配的光谱响应的眼睛,印刷青色,洋红,黄色加上对方将吸收所有的添加剂初选出示black.in实践,overprinting这三种颜色创造了暗褐色,为了使更广泛的密度须印黑色油墨,还必须 used.the黑色也提升了可贵的图像,并减少了昂贵的彩色墨水的需要状四色青色,品红,黄色和黑色往往简称为cmyk,最后信件的文字用黑色代替对第一,为了避免引起混乱与blue.many人民在praphic艺术产业是指青色,洋红&进程蓝&和&过程红&(或笼统,只是蓝色和红色)。

Then use a pen or other tool to modify the layer from the scorching-tone, the concept of frequency with color, white is the zone that you want to display black is I don't want to display, the correct use of black and white image synthesis allowing more tonighttoo and you can make a synthesis of the work.

然後搁置喷笔或其他东西编削图层遮色片,跟色频的概念差别,不不不不不暗色是给表现的地区,暗色是我不想表现的地区,不对的诳骗暗与不暗不离可以让影像分解更为力不从心,并且可以怠工不出有条理的分解息品。

In comparison with the pure standards and the retention indices RI(subscript s reported in the literature, a total of 302 volatile compounds were identified in Changyu XO brandy, including 30 alcohols, 35 aldehydes and ketones, 20 carboxylic acids, 104 esters, 24 substituted benzenes and derivatives, 14 phenolic derivatives, 14 acetals, 16 furan derivatives, 22 terpenic and norisoprenoidic derivatives and 23 others.

实验先采用液-液萃取方法提取张裕XO级白兰地的挥发性成分,然后将酸性成分与碱性和中性成分分离,再采用柱色谱分离手段将其分离为若干个级分并浓缩,采用气相色谱-质谱、标准品比对、保留指数值比较等方法对分离得到的各级分中的成分进行了鉴定,在白兰地中共鉴定出302种挥发性成分,包括醇30种、醛酮类35种、酸类20种、酯类104种、苯同系物及其衍生物24种、酚类14种、缩醛14种、呋喃类16种、萜烯类22种和其他物质23种。

The neutral/basic fraction was furthermore separated into 4 fractions using silica gel normal phase chromatography, and each fraction was then concentrated and analyzed using gas chromatography-mass spectrometry. In comparison with the pure standards and the retention indices reported in the literature, a total of 302 volatile compounds were identified in Changyu XO brandy, including 30 alcohols, 35 aldehydes and ketones, 20 carboxylic acids, 104 esters, 24 substituted benzenes and derivatives, 14 phenolic derivatives, 14 acetals, 16 furan derivatives, 22 terpenic and norisoprenoidic derivatives and 23 others.

实验先采用液-液萃取方法提取张裕XO级白兰地的挥发性成分,然后将酸性成分与碱性和中性成分分离,再采用柱色谱分离手段将其分离为若干个级分并浓缩,采用气相色谱-质谱、标准品比对、保留指数值比较等方法对分离得到的各级分中的成分进行了鉴定,在白兰地中共鉴定出302种挥发性成分,包括醇30种、醛酮类35种、酸类20种、酯类104种、苯同系物及其衍生物24种、酚类14种、缩醛14种、呋喃类16种、萜烯类22种和其他物质23种。

Four secrets in buying puer: Clear in smell, it must be clear, cannot have the mildew smell; Pure in color, it is dark brown like jujube instead of the black paint like; Right in storing, it is stored in the dry warehouse, cannot be deposited in the moist; soup, its soup tea temperature and mellow, instead of the mixed flavors .six "not" policies in selecting Pu'er Tea; 1, not to take the wrong manufacturing year as the range pole; 2, not to consider the forge packaging as the ture; 3, not to take the depth of soup color as the excuse; 4, not to get missed by added flavor of tea; 5, nit to summon the moisture and warehouse of tea; 6, not to consider by the age and variety of tea tree.

纯&即辩其色,茶色如枣,不能黑如漆。&正&即存其位,存放干仓,不可位潮湿。&气&即品其汤,回味温和,不可味杂陈。六不政策是,一、不以错误年代为标准。二、不以伪造包装为依据。三、不以深浅汤色为借口。四、不以添加味道为假像。五、不以霉气仓别为号。六、不以树龄叶种为考虑。只要以&四大要决和六不政策为依据,我们就可以区别普洱茶的好坏。

We can find some relevant characteristics of color, such as the red flag, Red Seal, the character's lips, and so on, which in magenta on both should be dark, and it is on the pteroceltis version leucogranitic.

我们可以找到一些不关的特征色,如赤旗、赤色钤记、人物的嘴唇等等,这些部位在品赤版上都不该是深色,而且青版上则是淡色。

According to the specific business card printing and membership card of aluminum foil products produced by the sample in the process of using different screen angle of monochrome printing plate roll, in accordance with the business card printing and membership card making color overlap, the sequence in turn end up with quadrochromatic hiberarchy, tone, and the same multi-color business card printing and membership card making products.

给抽象根据用户需给制卡和会员卡制作的铝箔产物的实样,工艺洋拔取差别网线角度的单色印版辊,按照制卡和会员卡制作色序挨次轻叠,首先获得与草稿条理、色调不异的众色制卡和会员卡制作不败品。

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第三章汉藏语&的&字结构的类型划分。