致病酶

①Location of WD gene in Ch inese: Using pairwise linkage analysis and multipoint linkage analysis method, w e constructed a genetic map of DNA markers within D13q14.2-3 which refined the location of WD gene by restriction fragment length polymorphism and microsatellite polymorphism analysis;②Screen for mutations of WD gene in Chinese people: we detected the structure of 21 exons of WD ge ne in 45 patients from 39 pedigrees by PCR-SSCP(Single strand conformation poly morphism) and PCR-DNA sequencing technology, found a new mutation in exon 5 and nuclcotide sequence analysis showed it is a T insertion. We also conformed the Arg778Leu in exon 8, the highest frequence mutation point in Chinese people, wit h mutation rate 22.8%in total;③Carrier detection and presymptomatic diagnosi s of WD: Based on DNA recombination technology, we peformed successfully the gen e diagnosis in all individuals of 79 families with WD and built up a helpful spe cific enzyme cut method (PCR-Msp1) to detect the carrier and presympomatic patients in Chinese pe ople with WD.

①WD的基因定位研究：通过RFLP及微卫星多态性分析，应用两位点及多位点连锁软件，建立了中国人WD基因在D13q14.2-3区域的精细遗传连锁图谱，从而首次对中国人WD基因进行了精确定位；②WD基因突变研究：应用PCR-SSCP及DNA测序技术，对39个家系45名WD患者进行该致病基因的21个外显子突变筛选，发现WD基因5号外显子存在新的T插入突变，并证实中国人WD基因的突变热点为8号外显子，突变形式为Arg778Leu，其频率为22.8%；③WD的症状前诊断和杂合子检出：应用DNA重组技术对79个家系进行基因诊断，成功地进行了WD的症状前诊断和杂合子检出，并建立了WD的基因筛选的PCR-Msp 1酶切方法。

Nov.. The main symptoms of the bacterial disease on Vigna angularis were canker of stalk.The strains were straight or curved rods; polar flagellum one; Gram-negative; could infect Vigna angularis、 Glycine max、 Vigna radata、 Vigna umbellate、 Phaseolus vulgaris、 Pisum sativum、 Pisum sativum var.humlie; oxidase negative; nitrate reduction negative; 5% glucose agar mucilage positive; esculin hydrolysis positive; starch negative; milk hydrolysis positive; H_2S positive; acid from L-arabinose, D-mannose, D-galactose, cellobiose, trehalose and fructose; 5%NaCl positive; G+Cmol%-69%.

红小豆溃疡病主要症状为茎部溃疡；各菌株菌体均杆状；极生鞭毛1根；革兰氏染色阴性；人工接种能侵染多种豆科植物；好氧；氧化酶阴性；硝酸盐反应阴性；5％葡萄糖营养琼脂黏液生长阳性；七叶灵水解阳性；淀粉水解阴性；牛奶分解产碱；产生硫化氢；能从阿拉伯糖等产酸；最大耐盐量为5％；G+Cmol％为69％等特征，根据《手册》9th ed相关部分，确定该病由野油菜黄单胞菌苜蓿致病变种Xanthomonas campestris pv.alfalfae(Riker，Jones et Davis 1935)Dye 19781引起。

The results showed that when the proportion of the resistant subpopulation within the h-VRSA was low, te level of the toxic factor and its pathogenicity to animals had no difference from that of the standard strain. If proportion of the resistant subpopulation increased the level of coagulase and lecithinase changing from positive from negative and the ring of hemolysis disappeared. The speed of proliferation of the organisms in the animal slowed down and the LD50 increased.

结果 当试验菌株中耐药亚群的比例较少时，其毒力因子及动物致病性检测结果与金葡菌标准菌株无显著差异；随着试验菌株中耐药亚群的比例增加，表现为血浆凝固酶、卵磷脂酶由阳性转为阴性，血琼脂平板上溶血环消失，细菌在动物体内增殖速度减慢，对动物的半数致死量(LD50)增加。

The liqase was positively controlled by HAI and AI-2 to some extent. The lecithinase was negatively controlled by HAI-1, and the hemolysin was negatively controlled by both HAI-1 and AI-2. Special primers of luxS gene were designed from the luxS gene sequence of V. harveyi obtained from the GenBank, and the gene was amplified from a pathogenic strain V. harveyi VIB645 by PCR. The luxS gene was cloned to a cloning vector pUC, and was sequenced. It was found that the open reading frame is 519 bp. The similarity of the luxS gene from V. harveyi VIB645 to the published sequence of V. harveyi strain in GenBank was only 90%.

从GenBank中获取哈维氏弧菌中编码AI-2合成酶的luxS基因序列设计一对特异性引物从哈维氏弧菌VIB645中PCR扩增luxS基因，将luxS基因克隆于pUC载体并测序，发现其与GenBank中已发表的哈维氏弧菌的luxS基因序列相似性为90%，证明LuxS/AI-2系统在哈维氏弧菌不同的菌株中的具有一定的特异性，推测LuxS/AI-2系统可能与哈维氏弧菌的致病性或发光现象有关。

They are not only involved in the process of fungal development and differentiation, such as supplying nutrition, for the growth of mycelium, attaching themselves to the morphosis and alteration of the cell wall of hyphae, as well as taking part in autolysis of filamentous fungi, but also show an important role in the process of pathogenesis including touching and cling on to the cell wall o.

1，3-葡聚糖酶(β-1，3-glucanase)是一类能够水解β-1，3-葡萄糖苷键的酶，普遍存在于多种真菌中，它们不仅在真菌生长发育起重要作用，如为菌丝体生长提供营养、参与菌丝体细胞壁的形态建成和构象的改变、参与菌丝体自溶等，而且在病原菌入侵寄主植物与致病的过程中扮演着重要角色，如：参与接触和吸附寄主细胞壁；破坏包围病原菌入侵结构的乳突。

Results: The 1034 strains of 8 types of pathogenetic aeromonase are sensitive to most of antibiotic and the highest resistance rate of 91.9% is ampicillin. The second one of 77.9% is piperacitlin and terramycin and sulfona mides are also with the high resistance rate of 62.4% and 56.9%. 127 strains (12.3%) which can produce the Ampc enzyme was found and 45 strains (4.4%) was multiple-drug resistance.

结果：8种1034株致病性气单胞菌对大多抗生素敏感，耐药率最高的是氨苄西林抗生素，达91.9%，哌拉西林次之，为77.9%，土霉素和磺胺类也有较高的耐药率，分别为62.4%和56.9%；检出具有产AmpC酶能力的致病性气单胞菌127株，占12.3%，多重耐药性菌株45株，占4.4%。

A deficiency in cholinergic neurotransmission is considered to be one of the major causes of memory impairments in patient.

致病机理之一是在神经冲动传输过程中缺乏乙酰胆碱，乙酰胆碱酯酶能分解乙酰胆碱，因而成为一个被广泛关注的靶分子。

Moreover, no significant changes in flagellar mobility, biofilm formation and production of extracellular cellulase and xylanase in vitro were observed in △rbfCxoo compared to PXO99A. Most importantly, the deletion mutation of rbfCxoo resulted in enhanced virulence and gene expression.

此外，△rbfCxoo鞭毛运动性、生物膜形成和胞外纤维素酶和木聚糖酶活性都无明显改变，但对水稻的致病性显著增强，毒性相关基因的表达也有所增加。

Objective To establish an initial system of identification of Leptospira in China by mapped restriction sites polymorphisms of rRNA gene.

目的 初步建立我国致病性钩端螺旋体rRNA基因限制性内切酶酶切位点多态性分类体系。

Objective To establish an initial system of identification of Leptospira in China by mapped restriction sites polymorphisms of rRNA gene.

目的初步建立我国致病性钩端螺旋体rRNA基因限制性内切酶酶切位点多态性分类体系。

Finally, according to market conditions and market products this article paper analyzes the trends in the development of camera technology, and designs a color night vision camera.

最后根据市场情况和市面上产品的情况分析了摄像机技术的发展趋势，并设计了一款彩色夜视摄像机。

Only person height weeds and the fierce looks stone idles were there.

只有半人深的荒草和龇牙咧嘴的神像。