致病酶

The pathogenic mechanism of toxin from Phoma herbarum to dayflower was studied through measuring cell membrane permeability, MDA content and activities of CAT, POD and APX. The result indicated that toxin increased permeability of cell membrane.

以草茎点霉毒素对鸭跖草叶片组织细胞膜透性、丙二醛含量、过氧化氢酶、过氧化物酶以及抗坏血酸过氧化物酶活性的影响，研究草茎点霉毒素对鸭跖草的致病机理。

An h-VRSA strain isolated from clinical specimen was induced to increase its resistance to vancomycin in vitro. The coagulase, lecithinase, hemolysin of this resistant strain and its pathogenicity to laboratory animals were examined in comparison with that of the standard S. aureus ATCC29213 strain.

使用万古霉素体外诱导方法将h-VRSA在体外逐渐诱导为VISA，通过对侵袭性酶、毒素的检测及动物致病性观察，并与金黄色葡萄球菌标准菌株比较，了解其致病性的变化规律。

And PG activities of pathogenic isolate was obviously higher than that of nonpathogenic isolate, It is same that PG activities of all isolates were the highest in the seventh day after cultivating.

综上所述，PG活性的高低与大豆疫霉根腐病菌的致病力强弱有一定的关系，是该病菌的一种起致病作用的细胞壁降解酶。

Methods Real-time PCR, using the primer-probe specific for the yst gene, was applied to detect the pathogenic （7 serotypes） and nonpathogenic （ 5 serotypes） Yersinia enterocolitica, other species of Yersinia （8 types）, and other enteric bacteria （8 types）.

应用针对致病性小肠结肠炎耶尔森菌染色体上yst基因而设计的特异引物和探针进行实时定量聚合酶链反应，检测致病性（7种血清型）和非致病性（5种）小肠结肠炎耶尔森菌、耶尔森菌属内的其他菌种（8种）及其他肠道细菌（8种）。

The results showed:among the 7 isolates, five isolates of HY3、GY1－3、ZJ1－1、HP1、FC3 had same colony shape, irregular shape, liquidlike, slimy, opacity with smooth surface;the other two isolates had same shape, irregular shape, dry, opacity with coarse surface. By inoculating eucalyptus with the 7 isolates, the plants were infected apparently, and the young plants of eucalyptus in control experiment with tap water were not infected. By cultivating eucalyptus cuttings with the bacterial suspensions without EPS, the incidence of disease was very distinct,but compared with the former bacteria suspension,the incidence of disease has decreased at different degrees. By screening out two isolates of strong pathogenicity and two isolates of weak pathogenicity from the 7 isolates,making the bacterial suspensions with them to inoculate the young plants of eucalyptus, two treatments of cutlings and ramets with rats were set with 5 repetitions in every treatment, the results of data analysis showed: for the cutlings, the bacterial contents in upper and middle parts、upper and lower had significant difference;for ramets with roots, the bacterial contents in upper, middle parts, lower had significant difference between each other; For both the cutlings and ramets with roof, the bacterial contents in xylem and phloem had significant difference. The interaction between vertical and horizontal parts for the bacterial content had significant difference. For the two isolates of HY3 and 93B which were screened out at last,their activities of the cellulase were: 1.955ug/ and 1.288ug/ respectively, and had significant difference; the activities of pectase were: 1.325 ug/and 1.24ug/ respectively, and had no significant difference. The content of EPS extracted from the two isolates of HY3 and 93B was very different: 7.08x10-8ug/cell and 5.17x10-8ug/cell.

结果显示：7个菌株中，其中5个菌株HY3、GY1－3、ZJ1－1、HP1、FC3的菌落形态相同：不规则形状、流体、粘性、不透明、表面光滑；另外2个菌株93B、GN1菌落形态相同：不规则形状、干燥、不透明、表面粗糙；用7个菌株接种剪根桉树苗，发病情况非常明显，而自来水对照实验中桉树苗却不发病；无EPS菌悬液培养桉树剪根苗，发病率也很明显，但是相比原菌液，则发病率有不同程度的下降；从7个菌株中间筛选出来2个强致病性菌株和2个弱致病性菌株，用它们配制菌悬液培养桉树苗，设置剪根和不剪根两个处理，每个处理设置五个重复，数据分析结果显示：对于剪根苗，上部和中部、上部和下部的含菌量有显著的差异，中部和下部含菌量差异不显著；带根苗，上部、中部、下部含菌量彼此之间差异显著；不管是剪根苗还是带根苗，木质部和韧皮部含菌量之间的差异都非常显著；上中下与木韧交互作用中，含菌量差异非常显著；最后筛选出来的强弱2个菌株HY3和93B，它们的纤维素酶活性分别为：1.955ug/和1.288ug/，具有显著的差别；果胶酶的活性分别为：1.325 ug/和1.24ug/，没有显著的差别，而且HY3和93B两个菌株细胞分泌的胞外多糖的含量差异很显著，分别为：7.08×10-8ug/cell和5.17×10-8ug/cell。

Virulence test demonstrated that the recombinant AHL-Lactonase has a strong anti-pathogenic activity to Erwinia cawtovora, and the addition of AHL-Lactonase to the medium of pathogen could reduced the activity of pectate lyase, prctin lyase, cellulase and polygalacturonase after 20h.

致病性检测表明，重组AHL-Lactonase对胡萝卜软腐病菌具有较强的抗病活性，在致病菌培养基加入AHL-Lactonase培养20h之后，培养基上清之中果胶酶(pectate lyase and prctin lyase，PL)、纤维素酶(cellulase，Cel)和多聚半乳糖醛酸酶(polygalacturonase，PG)的活性都存在不同程度的减小。

Five kinds of bacteria were isolated from disease Meretrix meretrix and culture water (two isolated from the M. meretrix, three from the culture water) in Lvsi harbor, Jiangsu province. The results of infection showed that strain WG1702 can cause disease with the mortality rate 100 percent and the same symptoms as original. It was cued that strain WG1702 was the pathogenic bacteria and caused the death in large scare, a preliminary research was done on the bacteria including morphological, physiological, biochemical characteristics, phylogenetic position and pathogenic. Strain WG1702 was a straight-rod gram negative bacterium, the physiological and biochemical indicators were lysine decarboxylase, ornithine decarboxylase, reduction of NO3- to NO2-, mannitose, oxidase and methylred reaction positive, arginine decarboxylase, arginine dihydrolase, salicin and voges-proskauer negative.

本文从江苏吕泗文蛤养殖区发病文蛤和养殖水体中分离到5株优势菌(病蛤中分离到3株优势菌，养殖水体中分离到2株优势菌)，人工感染实验结果显示，菌株WG1702能引起供试文蛤发病，死亡率为100%，且发病症状与原发病症状相同，提示该菌是引起文蛤大面积死亡的主要致病菌，对其形态、生理生化特征、分类地位及致病性进行了初步研究，菌株WG1702为革兰氏阴性菌，直杆状，生理生化指标为：赖氨酸脱羧酶、鸟氨酸脱羧酶、硝酸盐还原、甘露糖、氧化酶、甲基红阳性，精氨酸脱羧酶、精氨酸双水解酶、水杨素、V.P阴性。

For increasing our knowledge on the pathology of entomopathogenic nematodes to ticks, the changes of total protein level, esterase activity and esterase bands in the hemolymph of female Haemaphysalis longicornis Neumann after infection by the nematode Heterorhabditis bacteriophora E-6-7 (Hb E-6-7) were detected.

用嗜菌异小杆线虫Heterorhabditis bacteriophora E-6-7 (Hb E-6-7)感染长角血蜱Haemaphysalis longicornis雌蜱，测定感染后雌蜱血淋巴总蛋白含量、酯酶活性及酯酶同工酶的变化，以探讨昆虫病原线虫对蜱的致病机理。

We detected the distribution of lipases in 35 Aeromonas isolates by PCR andphenotypic test using tributyrin as substrate. The positive rates were 100% and 83%,respectively. The lipase activity or associated genes could be detected in the two avirulentstrains, A. hydrophila W1 and A. hydrophila 4332. It suggested that lipase might not be animportant factor in pathogenic mechanism of A.

对本实验室保存的35株气单胞菌分离株进行了脂酶的表型检测和PCR检测，结果分别为83％和100％，特别是嗜水气单胞菌无毒株W1和Ah4332也能检测到脂酶活性或基因，表明脂酶在该菌的致病机制中不是决定性因子。

It is caused by mutations in dyskerin-encoding genes, telomerase-encoding RNA component genes and reverse transcriptase genes, as well as other uncharacterized genes. There are three inherited forms, including X-linked, autosomal dominant and autosomal recessive inheritance.

先天性角化不良是与编码角化不良蛋白基因、编码端粒酶的RNA组份基因、编码端粒酶的逆转录酶基因突变及其他未确认的致病基因突变引起的基因病，其遗传方式有：X-性联隐性遗传、常染色体显性遗传及常染色体隐性遗传。

Finally, according to market conditions and market products this article paper analyzes the trends in the development of camera technology, and designs a color night vision camera.

最后根据市场情况和市面上产品的情况分析了摄像机技术的发展趋势，并设计了一款彩色夜视摄像机。

Only person height weeds and the fierce looks stone idles were there.

只有半人深的荒草和龇牙咧嘴的神像。