致病

We identified the likely disease-causing mutation by applying genomic methodologies (multiplex target amplification and massively parallel sequencing) to historical specimens from the Romanov branch of the royal family.

我们从皇室家族Romanov一支取得样本，通过遗传学方法（多元靶点扩增和大量平行测序）确定了可能的致病性突变。

The apxⅢA gene of Actinobacillus pleuropneumonie was amplified by PCR. The amplified DNA fragment 3 466bp was cloned into pMD18-T. After R.E. analysis and sequencing, the apxⅢA gene in pMD18-T was ligated into pBluescripⅡSK, the recombinant expression plasmid pET-28b/apxⅢA was constructed and analysed with R.E., the protein of apxⅢA gene expressed in E.coli BL21 was detected by Western blotting.

因胸膜肺炎放线杆菌的致病性主要是由毒素决定的，故参照猪胸膜肺炎放线杆血清2型菌株的序列(GenBankL1 2 1 4 5)设计了一对特异性引物，用PCR的方法扩增apxⅢA基因并得到了长 3 466bp的片段，然后将其克隆到pMD 1 8T中，经酶切鉴定和序列分析表明克隆是成功的；再将apxⅢA插入到原核表达载体pET 2 8b后，转化BL2 1 (DE3)，在IPTG诱导下获得高效表达，经Westernblotting检测证实表达产物有活性。

This review foused on Newcastle Disease Virus on genemic structure, structure protein and their function, the essence of pathogenesis, molecular diagnosis, and its vaccine research.

本文就对 NDV的分子生物学特征、结构蛋白及其功能、NDV的致病本质、分子诊断技术和疫苗研究等方面进行了较为详尽的综述

"Inasie candida infections are a major cause of morbidity and mortality in preterm infants," write Paolo Manzoni, MD, from the Sant'Anna Hospital in Turin, Italy, and colleagues from the Italian Task Force for the Study and Preention of Neonatal Fungal Infections and the Italian Society of Neonatology.

都灵的Sant'Anna医院Manzoni医学博士和为&新生儿真菌感染预防和研究&而来的意大利特遣部队的和意大利新生儿学会的的同事们写道：&致病性的念珠菌感染是早产儿中发病率和死亡率的主要原因。&

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。