致病

ABSTRACT Objective To investigate the distribution and antimicrobial susceptibility of pathogenic Vibrio in brine of Southeast China Sea area.

目的 了解我国东南沿海海水中致病性弧菌的分布情况及其对抗菌药物的敏感性。

This investigation would be of great significance for prevention and therapy of bacteria infections in brine.

海水中致病性弧菌分离及抗菌药物敏感性分析，对海洋弧菌所致疾病的预防、诊断和治疗都具有重要指导意义。

This investigation will be of great significance for prevention and therapy of bacteria infection in brine.

海水中致病性弧菌分离及抗菌药物敏感性分析，对海洋弧菌所致疾病的预防、诊断和治疗都具有重要指导意义。

It is apparent that the VirB8-PCR assay appears to be specific and sensitive and can be used for the detection of the virulence factor of Brucella as well as the surveillance of brucellosis.

VirB8-PCR方法特异、敏感，能用于布鲁氏菌病的监测和致病力因子的检测。

To develop a method to detect the virulence factor of brucells for use in diagnosis and surveillance of brucellosis.

目的 建立VirB8-PCR方法，用于布鲁氏菌病的诊断和致病力因子的检测。

Cotton dust is the cause of a disease called byssinosis, more commonly known as "brown lung" disease

棉屑是棉屑沉着病的致病原因。

Weather ignore cold heat up suddenly, suddenly shade suddenly fine, fantasticality, because this is easy have a recurrence of an old illness, add climate calefacient, easy breed of bacterium, virus, make human body causes disease.

天气忽冷忽热，乍阴乍晴，变化无常，因此容易旧病复发，加上气候变暖，细菌、病毒易繁殖，使人体致病。

To diagnose pre-symptomatic patient and detect potential carrier by WD gene diagnosis based on gene mutation high frequency spot screening.

通过突变热点检测进行WD基因诊断和早期诊断，筛查症状前患者和无症状的致病基因携带者。

①Location of WD gene in Ch inese: Using pairwise linkage analysis and multipoint linkage analysis method, w e constructed a genetic map of DNA markers within D13q14.2-3 which refined the location of WD gene by restriction fragment length polymorphism and microsatellite polymorphism analysis;②Screen for mutations of WD gene in Chinese people: we detected the structure of 21 exons of WD ge ne in 45 patients from 39 pedigrees by PCR-SSCP(Single strand conformation poly morphism) and PCR-DNA sequencing technology, found a new mutation in exon 5 and nuclcotide sequence analysis showed it is a T insertion. We also conformed the Arg778Leu in exon 8, the highest frequence mutation point in Chinese people, wit h mutation rate 22.8%in total;③Carrier detection and presymptomatic diagnosi s of WD: Based on DNA recombination technology, we peformed successfully the gen e diagnosis in all individuals of 79 families with WD and built up a helpful spe cific enzyme cut method (PCR-Msp1) to detect the carrier and presympomatic patients in Chinese pe ople with WD.

①WD的基因定位研究：通过RFLP及微卫星多态性分析，应用两位点及多位点连锁软件，建立了中国人WD基因在D13q14.2-3区域的精细遗传连锁图谱，从而首次对中国人WD基因进行了精确定位；②WD基因突变研究：应用PCR-SSCP及DNA测序技术，对39个家系45名WD患者进行该致病基因的21个外显子突变筛选，发现WD基因5号外显子存在新的T插入突变，并证实中国人WD基因的突变热点为8号外显子，突变形式为Arg778Leu，其频率为22.8%；③WD的症状前诊断和杂合子检出：应用DNA重组技术对79个家系进行基因诊断，成功地进行了WD的症状前诊断和杂合子检出，并建立了WD的基因筛选的PCR-Msp 1酶切方法。

Error catastrophe theory has led to a strategy of mutagenesis to control disease viruses.

错误突变理论引导了通过突变来控制致病病毒的策略。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。