致死剂量

In this study, for the complicated distribution of dose rate in time and space, considering the repair of the sub-death cells, the radionuclide decay law and exponential proliferation of clonogenic cells, the dose-response relationship is built based on the known linear-quadratic model, and the concept of tumor control probability is introduced into the therapy method. Furthermore, the concepts about "curable stage" and "critical time point" and the methods calculating the amount of radionuclide injected into tumor in clinical are proposed.

本课题针对这一方法中靶区复杂的剂量率时-空分布特点，以公认的"线性-二次模型"理论为基础，同时考虑亚致死性细胞修复、核素衰变规律和克隆原性细胞指数增殖，建立了适合这一治疗方法的具有时-空分布特点的剂量效应关系，并进一步将肿瘤控制概率的概念引入这一方法中，提出了"治愈期"及"临界时间点"的概念以及由此指导临床确定药物注射剂量的方法。

To verify whether it was safe or not in food processing, an acute toxicology experiment was carried out. The results of Horn's Method showed that LD50 of the endoenzyme extraction was higher than 21500mg/kg and thus was classified as no toxicity, while the microbe cells hadn't begotten the acute toxic effects with the biomass less than 1.72×109cfu/ml.

为了进一步研究其安全性，选用霍恩氏法测定半数致死量LD50，结果证明胞内酶提取液的经口LD50大于21500mg/kg，属于无毒级别，有必要进入后续阶段的毒理学试验；活菌制剂在1.72×109个/ml剂量以下不会引起急性毒性反应。

That the survival fractions of fractional irradiation were higher than that of single irradiation for all of the three kinds of cells and it indicated that in 6 hours all of the three kinds of cells had high repair ability to sublethal damage.

在0～6Gy的小剂量范围内，6h分次照射的细胞存活率明显高于单次照射。结论在6h之内，三种组织来源的肿瘤细胞都存在亚致死损伤修复。

Result That the survival fractions of fractional irradiation were higher than that of single irradiation for all of the three kinds of cells and it indicated that in 6 hours all of the three kinds of cells had high repair ability to sublethal damage.

结果 在0～6Gy的小剂量范围内，6h分次照射的细胞存活率明显高于单次照射。结论在6h之内，三种组织来源的肿瘤细胞都存在亚致死损伤修复。

When EMS was applied, it was found that 2.0% EMS resulted the highest microkernel rate (15.6‰). Treating the materials with 2.0% EMS for 4 hours could lead to semi-lethal. Cytological observations found that chromosome aberration phenomena such as single microkernel, double microkernel, three microkernel, chromosome bridge, resort chromosome and syncytium were presented in the mutants.

EMS诱变途径中，浓度为2.0 %处理时间为4 h的组合获得最高的微核率（15.6‰），EMS浓度为2.0 %处理时间为4 h的组合使材料达到半致死；细胞学的观察看到了单微核、双微核、三微核、染色体桥、滞留染色体和合胞体等染色体畸变现象，再生苗中发现了1棵变异株。60Co辐射的诱变途径中，20Gy的剂量使不定芽半致死，再生苗中发现了1棵变异株。

The results of our study show that (1)ionizing radiation was mutagenic to E.

3γ射线剂量率对三株菌的致死和致突变影响大剂量率强于小剂量率。

Methods:(1) Dissoluble PGN and CpG DNA were immobilized onto the surface of biotin cuvette for establishing target. Another effective tracking approach was established by immobilizing Escherichia lipid A F583 onto the surface of Non-derivatised cuvett. The biosensor technology was applied to screen anti-inflammatory TCM targeting on three key molecules.(2) The active compositions were isolated by AB-8 macroreticular resin from lycium bark. After the activities of compositions were evaluated, the most effective compositions was confirmed. In vitro, the affinities of different concentrations composition E binding with PGN, CpG DNA and lipid A were measured separately. The effect of composition E on vigor of RAW264.7 cells were tested by MTT and CCK-8, and its inhibition on TNF-α, which was released from RAW264.7 cells induced by PGN, CpG DNA and LPS, was also tested by ELISA. In vivo, murine sepsis models were made by intravenously heat-killed E.coli and heat-killed S.aureus, then protection of composition E on mice sepsis model were observed.

(1)将PGN及CpG DNA包被于生物素样品池，将lipid A包被于非衍生样品池，分别建立以PGN、CpG DNA及lipid A为靶点的技术平台，对114种抗炎中药水提物进行筛选、评价其活性物质含量，并评估出针对上述三种病原分子均具有较高结合活性的中药；(2)利用生物传感器跟踪检测技术、大孔吸附树脂分离技术，从地骨皮中定向分离与PGN、CpG DNA及lipid A均具有较高亲和力的活性组分；在体外实验中，测定不同浓度活性组分与PGN、CpG DNA及lipid A亲和力；MTT法及CCK-8法检测活性组分对RAW264.7细胞活力的影响；ELISA法检测活性组分对PGN(2μg/ml)、CpG DNA(10μg/ml)及LPS(100ng/ml)刺激小鼠RAW264.7细胞分泌TNF-α的抑制作用；在体内实验中，采用尾静脉注射致死剂量热灭活大肠杆菌和热灭活金黄色葡萄球菌，建立细菌脓毒症小鼠模型，观察活性组分对脓毒症模型小鼠的保护作用。

Methods: Lethally irradiated C3H/HeJ mice were reconstructed with syngeneic bone marrow cells and induced with cyclosporine for 21d.

用同基因骨髓细胞重建致死剂量X线照射的小鼠，用环孢素A腹腔注射诱导治疗21d。

The mice in experimental group and control group were exposed to 350 cGy radiation produced by 60Co. After 3 h, karyocytes at different concentrations in the fresh human umbilical cord blood were injected into the mice in experimental group A, B, C via their tail veins, and the equal volume of normal sodium was also injected into control group via tail veins. After one month, carbon tetrachloride (CCl4) was injected into experimental group A, B and control group via abdominal cavity, and the equal volume of normal sodium was injected into experimental group C. After two months, immunohistochemistry and reverse transcriptase polymerase chain reaction were used to detect the expressions of human cytokeratin-18 (CK18), cytokeratin-19 (CK19) and albumin in liver tissues of all mice.

采用60Co治疗仪γ射线对实验组和对照组行350cGy的亚致死剂量照射，实验组A、B、C3组照射后3h内经尾静脉分别输入1.0×10^7个/只、2.0×10^7个/只和3.0×l0^7个/只人新鲜脐血有核细胞，对照组经尾静脉注入等体积无菌生理盐水。1个月后对实验A、B组和对照组裸鼠经腹膜腔注射四氯化碳(CCl4)，实验C组注射等体积生理盐水。2个月后采用免疫组化和RT-PCR方法检测裸鼠肝组织人源性CK18、CK19和人源性白蛋白的表达。

Compared with before treatment, the enzyme activity of German cockroach changes with the difference of time after treated with sub-lethal doses of deltamethrin and phoxim.

经溴氰菊酯和辛硫磷亚致死剂量处理后德国小蠊体内酶活性在不同的时间与处理前存在差异。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。