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Coli BL21 and purified. The binding activities of immune sera to three EDs were examined with ELISA and Western blotting analysis.②Asthma model was induced with ovalbumin in mice of vaccine group, control vector group and asthma group. Airway pressure-time index and number of cells in BALF were checked. The quantity of goblet cells in bronchiole and the level of MUC5AC mRNA in lung were investigated by PAS staining and RT-PCR assay.

采用ELISA和Western blotting法检测免疫血清抗体对ED的亲合力。2、对DNA疫苗组小鼠制备OVA致敏哮喘模型,检测气道压力峰值—时间指数、支气管/肺泡灌洗液中炎性细胞数量,PAS特染观察小支气管杯状细胞数量及RT-PCR法检测肺组织中粘蛋白MUC5AC mRNA水平,免疫组化和RT-PCR法检测GM-CSF的蛋白与mRNA水平。

Summary Background: It has been shown that patients with allergic bronchopulmonary aspergillosis and patients with severe asthma with fungal sensitization can benefit from antifungal therapy.

背景:研究显示,抗真菌治疗可使变应性支气管肺曲霉菌病和真菌致敏性严重哮喘患者临床获益。

Objective: To explore the relation between allergen reaction and the pathogenesis of irritability rhinitis and bronchus asthma.

目的:比较单纯过敏性鼻炎患者和哮喘患者致敏原的阳性率,分析机体对过敏原反应程度与疾病发生、发展的关系。

Peanut being served as a model of oil-plants (such as soybean, sesame seed, sunflower seed, et al) and nuts (such as hazelnut, almond, pistachio, cashew nut, walnut), our research project is aiming for developing a practical method for quality control of food allergen preparation and providing identifying standards.

花生作为油料作物和坚果类食物(如核桃、榛子、杏仁、开心果、腰果等)的代表,上述有关花生变应原的抗原性及致敏组份的研究,其目的在于摸索并提供可行的食物变应原的质量评价方法,为促进食物变应原特别是花生变应原的标准化提供科学的参考依据。

Background: In occupational asthma continued workplace exposure to the causative agent is associated with a poor prognosis.

职业性哮喘患者持续暴露于工作场所的致敏因子与不良预后密切相关。

When the mice pre - sensitized were infected with RSV, the pulmonary inflammation, lymphocyte and eosinophils infiltration and cell - collar peribronchiles were more severe in lung organization and bronchiole than those in the single RSV infection group.

结果病理检查证实小鼠感染RSV后发展为典型的间质性肺炎;致敏小鼠感染RSV后病变更明显,细支气管周围淋巴细胞浸润形成管套增厚,嗜酸细胞增多,炎性细胞浸润的细支气管比例显著增高。

We hypothesised that Candida colonisation promotes food allergy, which is known to contribute to a pathogenic response in atopic dermatitis.

我们假设念珠菌定植可促进食物的致敏作用,这一作用导致特应性皮炎的致病反应。

And each group had 10 rats. After sensitization by injecting ovalbumin and inducing asthma successfully, different preventive and curial treatments were given to them for 6 days.

卵白蛋白致敏并诱喘成功后,各治疗组分别予内服桂龙咳喘宁、地塞米松和穴位贴敷"咳喘宁"贴膏进行治疗。

Results: the Result showed that compound Daihuang ointment has no acute toxicity, stimulative, and hypersusceptibility reaction to difference species animal, either in full cutis or injured cutis.

结果:复方大黄青对健康SD大鼠完整皮肤及破损皮肤均无急性毒性反应;对健康白兔完整皮肤和破损皮肤无刺激;对健康豚鼠完整皮肤无致敏作用。

According toGB/T 16886.10-1997 Biological Evaluation of Medical Apparatus and Instrument, compared with normal sodium, pure magnesium and medical Ti-6Al-7Nb, the leaching liquor were injected into cutis to evaluate stimulus response,to evaluate sensitivity response by injection and illinition.

采用与生理盐水、高纯镁及医用钛合金Ti-6A1-7Nb对照的研究方法,将镁合金浸提液注入豚鼠皮肤内观察皮内反应:采取皮内注射和涂皮相结合的诱导法观察豚鼠致敏反应。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

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