致敏

D immunoglobulin is administered to Rh-negative,unsensitized patients to prevent isoimmunization.

为避免同种免疫，给未致敏的Rh血型阴性患者注射D免疫球蛋白。

Simulate the condition of temperature and energy control in percutanous MWA for HCC to ablate SMMC-7721 cell strain suspension.There were five experiment groups:A.control group,B.45℃10 min group,C.50℃3min group,D.54℃3min group and E.60℃instant group.Detection for apoptosis was performed at 30 minutes and 2 hours after ablation.2.Thirty-two C57 mice were undertaken hypodermic inoculation of Hepal-6 tumor piece at right groin as ablation tumor and at left groin as observation tumor one week later.The mice with double tumors were separated into four groups:A.control group with no treatment,B.MWA group treated with MWA only,C.mDC group treated with MWA and subcutaneous injection of mature dendritic cells sensitized by neoantigen of Hepal-6 cell strain,D.iDC group treated with MWA and peritumoral injection of immature dendritic cells without neoantigen sensitization.

模拟肝癌微波治疗中常用消融区边缘的温控和能量条件，消融人肝细胞癌株SMMC-7721细胞悬液，分为五组：A、对照组，B、45℃10min组，C、50℃3min组，D、54℃3min组，E、60℃即刻组，消融后30分钟及2小时进行凋亡检测。2.32只C57小鼠左侧腹股沟皮下接种Hepa1-6鼠肝癌细胞瘤块作为消融瘤，间隔1周，右侧腹股沟皮下接种瘤块作为观察瘤，成瘤后分为四组：A组，对照组，不行治疗；B组，微波组，仅微波消融小鼠消融瘤；C组，成熟DC组，微波消融小鼠消融瘤，并于小鼠双侧腋下注射肿瘤抗原致敏的树突状细胞；D组，未成熟DC组，微波消融小鼠消融瘤，并于消融瘤周注射未成熟树突状细胞。

METHODS.: We tested sera from six in vivo ATG-treated kidney transplant patients after measuring serum concentrations, as well as six nonsensitized sera with ATG added in vitro.

对6例在血清浓度测量下进行体内ATG治疗的肾移植病人的血清进行化验，同时对6例体外实验加入ATG的非致敏的血清进行化验。

Based on relative literatures,this part has included five resesearch aspects as below.1 Fifteen compounds were isolated and purified by extraction,column chromatography,and their structures were determined on the basis of spectral analysis: acteoside(Ⅰ-1),isoacteoside(Ⅰ-2),crenatoside(Ⅰ-3),cistanoside F(Ⅰ-4),sinapoyl-4-O-β-D-glucoside(Ⅰ-5),adenosine(Ⅰ-6),β-siterol(Ⅰ-7),oleanic acid(Ⅰ-8), succinic acid(Ⅰ-9),caffeic acid(Ⅰ-10),protocatechuic aldehyde(Ⅰ-11),p-hydro xybenzyl alcohol(Ⅰ-12),β-daucosterol(Ⅰ-13),D-galacitol(Ⅰ-14),D-mannitol(Ⅰ-15).Ⅰ-4～15 were obtained from this plant for the first time,andⅠ-6,7,9,and 13 were isolated from Orobanche genus for the first time.2 The scavenging test of DPPH showed that most compounds have comparative antioxidant activity as L-ascorbic acid and part of them show better activity such as the O.coerulescens extract and phenylethanoid glycosides.Acteoside showed potent free radical scavenging effects with a median inhibition concentration of 25.6μg/ml.3 The anti-HBV activities of acteoside,isoacteoside and crenatoside were measured,and all of them showed suppressive activity on the expression of HBsAg and HBeAg in the HepG2.2.15 cell line.

本论文在文献调研基础上对紫花列当化学成分及生物活性进行了研究，并从免疫抗病毒角度探讨紫花列当中特征性成分类叶升麻苷的肝保护作用及其机制。1采用大孔树脂、硅胶和Sephadex LH-20等色谱技术对紫花列当进行系统的植物化学研究，从中分离得到19个化合物，利用UV和NMR等波谱手段及理化性质鉴定了其中的15个化合物，分别为类叶升麻苷(Ⅰ-1)、异类叶升麻苷(Ⅰ-2)、crenatoside(Ⅰ-3)、cistanoside F(Ⅰ-4)、sinapoyl-4-O-β-D-glucoside(Ⅰ-5)、腺苷(Ⅰ-6)、β-谷甾醇(Ⅰ-7)、齐墩果酸(Ⅰ-8)、琥珀酸(Ⅰ-9)、咖啡酸(Ⅰ-10)、原儿茶醛(Ⅰ-11)、对羟甲基苯甲酸(Ⅰ-12)、β-胡萝卜苷(Ⅰ-13)、D-半乳糖醇(Ⅰ-14)和甘露醇(Ⅰ-15除化合物Ⅰ-1、Ⅰ-2和Ⅰ-3外，其余化合物均为首次从该植物中分离得到。2)通过大孔树脂富集该药材有效部位苯乙醇总苷，并采用HPLC测得其所含特征性成分类叶升麻苷的含量可达80%以上。3DPPH自由基清除试验显示紫花列当提取物及其所含的苯乙醇苷类化合物均具有较好的抗氧化能力，其清除DPPH自由基能力接近于抗坏血酸。4采用卡介苗整体致敏、脂多糖离体攻击构建大鼠原代肝细胞免疫性损伤模型，在体外观察类叶升麻苷的保肝作用。

The sensitized T lymphocytes could promote osteoclastic differentiation and the ability of bone resorption.

致敏淋巴细胞能够促进骨髓破骨细胞的分化和骨吸收能力。

Caffeic acid phenethyl ester is a biologically active ingredient of honeybee propolis.

之前对咖啡酸苯乙酯的研究显示咖啡酸苯乙酯是具有抗病毒、抗发炎、抗氧化、放射线治疗的致敏剂等作用的性质。

Guinea pig sensitied by ovalbumin for model made and challenged with substance P were second group,and the last two groups were with steroid treatment and contral. Praxiology markers、 IgE、 histamine and SP were detected .Gene chip of rat were used in observing genes expression change.

再经卵蛋白致敏制作变应性鼻炎豚鼠模型，并按模型组，P物质干预组及类固醇激素治疗组及对照组分为四组，观察行为学指标，血IgE，组胺及SP含量的变化，通过鼠芯片观察基因表达谱及其变化。

Bronchial asthma in acute episode model was established by a manner of egg albumen sensibilization and provocation.

以卵白蛋白致敏、激发的方式建立支气管哮喘急性发作期模型

Bronchial asthma in acute episode model was established by a manner of egg albumen sensibilization and provocation. Rats in each group were treated by different drugs. Protein expressions of Fas/FaL-s in lung tissue were assayed by immune histochemistry.

以卵白蛋白致敏、激发的方式建立支气管哮喘急性发作期模型，各组分别给予相应治疗，免疫组化ABC法检测肺组织中Fas/FaL-s的蛋白表达。

The pathogenesis of asthma can be divided into three stages in immunology. The initial stage is sensibilization, which is closely associated with the activation of T cells. B7/CD28 and CD69 also play a significant role in this process. The second stage is chronic allergic inflammation. The immune dysfunction resulting from the disturbance of TH1/TH2 is a major cause of chronic allergic inflammation, and dominates asthma attack. And the third stage is airway remodeling due to chronic inflammation. Airway remodeling is one of the major causes of refractory asthma, which also contributes to the death from asthma.

哮喘的发病从免疫学角度可以分为三期：初期为致敏阶段，与T细胞激活关系密切，B7/CD28和CD69在这一过程也扮演重要角色；第二期为慢性过敏性炎症阶段，TH1/TH2失衡导致免疫功能紊乱是此阶段的主要原因，在哮喘发作过程中起主要作用；第三期为慢性炎症引起的气道结构重构阶段，是难治性支气管哮喘的重要发病和死亡原因。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。