致敏

The athymic recipients were reconstituted with 3.5 x 10(7) or 5 x 10(7) presensitized CD4+T-lymphocytes.

采用3.5×107或 5×107 CD4+T细胞预先致敏无胸腺的受者。

Results: BIO-1211 aerosol at 1, 3 and 10 mg/ml significantly inhibited the changes in lung resistance and lung dynamic compliance after antigen challenge in the sensitized rats in a dose-dependent manner, BIO-1211 at 25, 50, 100 and 200μg/ml inhibited the fibronectin-induced neutrophil adhesion by 23.5%, 24.6%, 61.4% and 58.1%, respectively, and serum-induced adhesion by 29.9%, 35.9%, 35.3% and 15.4%, respectively. Inhalation of 10mg/ml BIO-1211 did not show any protection against histamine and actetylcholin-induced bronchoconstriction.

结果：给大鼠气雾吸入BIO-1211 (1、3、10mg/ml)呈剂量依赖抑制致敏大鼠抗原攻击引起RL的升高和Cdyn降低；BIO-1211(25、50、100、200μg/ml)明显抑制纤维黏连蛋白和血清诱导的中性粒细胞黏附反应，其抑制率分别为23.5%、24.6%、61.4%、58.1%和29.9%、35.9%、35.3%、15.4%；但BIO-1211 (10 mg/ml)给豚鼠气雾吸入不能抑制乙酰胆碱和组胺诱导的药物性哮喘。

The application of CNI drug avoidance protocols to high-risk recipients (retransplants, highly sensitized, etc.), extrarenal allograft recipients, or alternative drug regimens such as steroid or MMF elimination should be subjected to controlled trials.

对于高危患者（再次移植、高度致敏等等）、除肾脏外其他器官移植患者、采用激素或MMF撤除的药物调整，该项避免CNI类药物应用的方案仍需要进一步对照实验。

Experimental results show that the proposed method can be used to prepare high titer and specific rabbit anti-Humulus pollen protein polyclonal antibody. It has similar ability in determining the composition of Humulus pollen allergic protein compared with sera of patients. Therefore, it can be used to immunoscreen cDNA library.

制备的兔抗血清具有较高效价和较好的特异性，应用该血清筛选出的葎草花粉致敏蛋白组分与患者血清筛选的组分相似，因此可进一步应用于初筛cDNA表达文库。

MethodsAccording toGB/T 16886.10-1997 Biological Evaluation of Medical Apparatus and Instrument, compared with normal sodium, pure magnesium and medical Ti-6Al-7Nb, the leaching liquor were injected into cutis to evaluate stimulus response,to evaluate sensitivity response by injection and illinition.

通过动物皮内刺激及致敏试验，对镁合金AZ91D(AZ0)、Mg-Mn-Ce(RE0)及其在三种不同阶跃式阳极氧化表面改性条件下所得新型镁合金(AZ1、AZ2、AZ3、RE1、RE2、RE3)的体内生物相容性进行初步的评价。

To evaluate the biocompatibility in vivo of Mg alloy AZ91D(AZ0),Mg-Mn-Ce (RE0) and their step anodizational materials (AZ1, AZ2, AZ3, RE1, RE2,RE3) by the experiments of intradermal stimulus and sensitivity response. According toGB/T 16886.10-1997 Biological Evaluation of Medical Apparatus and Instrument, compared with normal sodium, pure magnesium and medical Ti-6Al-7Nb, the leaching liquor were injected into cutis to evaluate stimulus response,to evaluate sensitivity response by injection and illinition.

通过动物皮内刺激及致敏试验，对镁合金AZ91D(AZ0)、Mg-Mn-Ce(RE0)及其在三种不同阶跃式阳极氧化表面改性条件下所得新型镁合金(AZ1、AZ2、AZ3、RE1、RE2、RE3)的体内生物相容性进行初步的评价。

Objective To investigate the influence of HLA immunogenic mismatching on acute rejection of renal transplants.

目的 探讨供受者HLA致敏原性错配对肾移植受者急性排斥反应发生率的影响。

Dendritic cell vaccine was impacted and sensitized by tumor associated antigen and antigen polypeptide in vitro, then was retransfused or immunogenically inoculated to tumour carrier host, can evoked specific CTL's antitumour immunoreaction.

应用肿瘤相关抗原或抗原多肽体外冲击致敏DC制成疫苗，回输或免疫接种于载瘤宿主，可诱发特异性CTL的抗肿瘤免疫反应。

In this study, oral tolerance was induced by tube-feeding transgenic mice with 0.5mg/day OVA protein daily, simultaneously sensitized by OVA protein with aluminum hydroxide. Oral-administered OVA protein (0.5mg/day for 5 or 10 consecutive days) resulted in significant lowered airway hypersensitivity as well as reduced inflammative cells infiltration, titer of serum OVA-specific IgE, IL-5 secretion in bronchoalveolar lavage fluid, and IL-4 secretion in splenocyte.

首先，本实验於小鼠中成功诱发了呼吸道过敏、大量OVA特异性IgE抗体生成、及嗜酸性白血球不正常聚集等现象；致敏前，同以连续管餵小鼠OVA抗原(0.5mg/day) 5天或10天后，结果显示皆能缓和呼吸道阻力、减低肺部发炎细胞浸润现象、降低血清中OVA特异性IgE抗体的生成量、也减少肺部冲洗液及脾脏细胞内第二型辅助T细胞(type II helper T cell， Th2)细胞激素的分泌(IL-4、IL-5)。

Animals subsequenting bearing 1 day were treated with four-weekinjections s.c of 1xl06 C26 tumor lysate-pulsed DC in the right inguen.

DC诱导的CTL杀伤实验：在效靶比为50：1，25：1，12.5：1，6.25：l时，对C26肿瘤细胞杀伤率分别为68.84%，58.36%，41.560，0，24.96%，而此eTL对B16肿瘤细胞杀伤作用很弱，说明肿瘤细胞冻融抗原冲击致敏的DC诱导的CTL具有抗原特异性。4。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。