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Some physicochemical therapy as well as nake hepatocytes transplantation can't correct metabolic disarrangement because of severe hepatic diseases, ALF is characteristic of more complications and easily resulting in multiple organs dysfunction , and even multiple organs failure as well as higher mortality. transplant technique of encapsulated hepatocytes can inhibit immune rejection, and quarantee hepatocytes survival, the technique provides a new therapeutic method for ALF.

由于肝脏功能较复杂,一些利用物理、化学方法及裸肝细胞移植来代替肝脏解毒功能,但不足以纠正严重的肝病所致的代谢紊乱,易致多脏器功能障碍甚至衰竭,并发症多,死亡率高;而微囊化肝细胞移植技术,既可阻断受体的免疫排斥反应,又保证移植的肝细胞在体内长期存活,为急性肝衰治疗提供新的治疗方法。

Prepare 131I-labbelled anti-CD20 monoclonal antibody using lodogen iodine labelling;(2)Measure the immunity activation of labbelled antibody by means of both cell combine analysis and LDH cytotoxicity detection kit;(3)Evaluate the injury rate of tumor exposed to 131I-chimeric anti-CD20 monoclonal antibody in vitro. The MTT method and the experiment of cell growth control are used;(4)Record the apotosis of Daudi cell using gelose electrophoresis;(5)Learn the inhibition effect of radioactive medication on the marrow. Radiohnmunoimages are taken on various intervals after injection of the labeled antibodies to the nude mice. The distribution of radioactive medication, I31l-labeled antibodies, in the marrow tissue indicates the inhibition effect. Here a B cell non-hodgkin lymphoma model in nude mice is established by us;(6)28-day observation are done in 3 groups of nude mice model.

(1) 用lodogen标记法制备131I-国产人鼠嵌合抗CD20单抗;(2)采用细胞结合分析法和乳酸脱氢酶法细胞杀伤性实验测定131I标记后国产人鼠嵌合抗CD20单抗与靶细胞的免疫活和利结合能力;(3) MTT法和细胞生长抑制实验测定131I-国产人鼠嵌合抗CD20单抗的体外杀瘤活性;(4)用琼脂糖凝胶电泳法研究131I-国产人鼠嵌合抗CD20单抗致Daudi细胞凋亡;(5)建立荷人B细胞淋巴瘤移植瘤裸鼠模型,应用γ计数法检测注射到荷瘤裸鼠体内的131I-国产人鼠嵌合抗CD20单抗的组织分布情况,明确其靶向性;(6)将荷瘤裸鼠分3组进行放射免疫治疗,分别为阴性对照组、131I-国产人鼠嵌合抗CD20单抗组、国产人鼠嵌合抗CD20单抗组。

Objective To study the relationship between DNA fragments in ENV gene of SIVmac and viral replicating ability. Methods Reciprocal recombinants were constructed by replacing the clonable DNA fragments of ENV region of the nonpathogenic SIVmac142 clone with the pathogenic SIVmac 239 clone.

猴免疫缺陷病毒(simian immunodeficiency viruses SIV)是一组在致病性和毒力方面具有多样性的灵长类动物病毒,其中,SIVmac239克隆感染的猴表现为与人类AIDS相似的疾病,称之为致病株,而SIVmac142克隆仅引起猴发生病毒血症,不发生临床综合征,称之为非致病株〔1〕。

METHODS:Guinea-pigs were sensitized by ovalbumin and the effects of LMWH on the constriction of ileum smooth muscle induced by OA were observed in vitro;The effects of LMWH on the degranulation of pericranium mast cell induced by OA were observed throu gh the passive anaphylaxis ratmodel which was made by vaccinating antiserum at pericranium;Licking response was induced by 4-aminopyridine(4-AP) scat scruff in mice,then the effect of LMWH on licking response was studied the capillary p emeabilty increasing induced by glactal acetic acid and the effect of LMWH on ca pillary pemeability were studied.

用卵白蛋白(ovalbumin,OA)免疫豚鼠造成过敏模型,观察LMWH对OA所致的豚鼠离体回肠平滑肌收缩的影响;在大鼠颅骨骨膜接种抗血清使肥大细胞被动致敏,观察LMWH对OA引起肥大细胞脱颗粒的影响;以4-氨基吡啶诱发小鼠舔体反应,观察LMWH对舔体反应的影响;以冰醋酸使小鼠毛细血管通透性增加,观察LMWH对通透性的影响。

YW could increase cellular immunity of carcinoma mice after radiation, reducing the side effects of immunity system caused by radiotherapy. At the same time, YW could reduce scathe caused by free radical.

YW能增强荷瘤小鼠放疗后的细胞免疫功能,减轻放射线对小鼠免疫系统的毒性,减轻放射所致的自由基损伤。

This may be the main cause of infections in the late stage of SCI.

随着时间的发展,大鼠的细胞免疫反应又逐渐恢复,这可能是机体免疫系统对脊髓损伤的刺激作出了反应所致。

Apoptotic cells have immunogenicity,and may be the major source of autoantigens in lupus BXSB mice.Lupus genetic factors and Yaa gene can potentiate the immunogenicity of apoptotic cells or accelerate their inducing the lupus nephritis.

凋亡细胞具有免疫原性,它可能是狼疮性BXSB小鼠体内自身抗原的主要来源;狼疮性遗传因素和Yaa基因能促进凋亡细胞的免疫原性和增强凋亡细胞的致肾炎作用。

Methods The present study has been carried out using NaCMC injected into the mice's abdominal cavity to cause inflammation and induce white blood cell migration,varnishing DNFB to induce mice's DTH,injecting colloid activated carbon suspension into mice's tail vein,with 10% of sheep RBC injected into the abdomen to immunize mice.We observed inhibitory function of Liyankang granule on NaCMC to cause inflammation and induce white blood cell migration,the effect of DNFB on inducing mice's DTH and the effect on clearance speed of carbon particle in mice's blood and serum agglution of mice.

采用大鼠腹腔注射羟甲基纤维素钠致炎诱发白细胞游走,涂抹二硝基氟苯诱导小鼠迟发型超敏反应,小鼠尾静脉注射胶体碳悬液,腹腔注射10%羊红细胞免疫小鼠,观察小儿利咽康颗粒剂对羟甲基纤维素钠致炎诱发白细胞游走的抑制作用,对二硝基氟苯诱导小鼠迟发型超敏反应、对小鼠血中碳粒清除速度、对小鼠血清凝集素的影响。

Methods The present study has been carried out using NaCMC injected into the mice's abdominal cavity to cause inflammation and induce white blood cell migration,varnishing DNFB to induce mice's DTH,injecting colloid activated carbon suspension into mice's tail vein,with 10% of sheep RBC injected into the abdomen to immunize mice.

采用大鼠腹腔注射羟甲基纤维素钠致炎诱发白细胞游走,涂抹二硝基氟苯诱导小鼠迟发型超敏反应,小鼠尾静脉注射胶体碳悬液,腹腔注射10%羊红细胞免疫小鼠,观察小儿利咽康颗粒剂对羟甲基纤维素钠致炎诱发白细胞游走的抑制作用,对二硝基氟苯诱导小鼠迟发型超敏反应、对小鼠血中碳粒清除速度、对小鼠血清凝集素的影响。

Department of Basic Medical Sciences,College of Vocational Technology,Liaoning University of TCM,Shenyang 110101.China Objective To observe immune regulation effect of Liyankang granule on children.Methods The present study has been carried out using NaCMC injected into the mice's abdominal cavity to cause inflammation and induce white blood cell migration,varnishing DNFB to induce mice's DTH,injecting colloid activated carbon suspension into mice's tail vein,with 10% of sheep RBC injected into the abdomen to immunize mice.We observed inhibitory function of Liyankang granule on NaCMC to cause inflammation and induce white blood cell migration,the effect of DNFB on inducing mice's DTH and the effect on clearance speed of carbon particle in mice's blood and serum agglution of mice.

采用大鼠腹腔注射羟甲基纤维素钠致炎诱发白细胞游走,涂抹二硝基氟苯诱导小鼠迟发型超敏反应,小鼠尾静脉注射胶体碳悬液,腹腔注射10%羊红细胞免疫小鼠,观察小儿利咽康颗粒剂对羟甲基纤维素钠致炎诱发白细胞游走的抑制作用,对二硝基氟苯诱导小鼠迟发型超敏反应、对小鼠血中碳粒清除速度、对小鼠血清凝集素的影响。

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In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国,慢性酒精中毒,肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题,你可以随时联系我。

Very pretty, but the airport looks more fascinating The other party wisecracked.

很漂亮,不过停机坪更迷人。那人俏皮地答道。