致免疫的

Coli BL21 and purified. The binding activities of immune sera to three EDs were examined with ELISA and Western blotting analysis.②Asthma model was induced with ovalbumin in mice of vaccine group, control vector group and asthma group. Airway pressure-time index and number of cells in BALF were checked. The quantity of goblet cells in bronchiole and the level of MUC5AC mRNA in lung were investigated by PAS staining and RT-PCR assay.

采用ELISA和Western blotting法检测免疫血清抗体对ED的亲合力。2、对DNA疫苗组小鼠制备OVA致敏哮喘模型，检测气道压力峰值—时间指数、支气管／肺泡灌洗液中炎性细胞数量，PAS特染观察小支气管杯状细胞数量及RT-PCR法检测肺组织中粘蛋白MUC5AC mRNA水平，免疫组化和RT-PCR法检测GM-CSF的蛋白与mRNA水平。

In addition, some of the failure of infectious coryza vaccination was due to the quality of vaccine and administration, and also some was due to the emergency of serotype B Haemophilus paragallinarum.

另外大多数鸡场的免疫失败是由于免疫效果不良所致，但也有相当一部分鸡场的免疫失败是由于B血清型的出现引起的。

For it is rubor after damaging to the closing soft tissue, getting swollen, getting hot aching and model of functiolaesa these bed solicit and there is no fracture,no skin damaged, we designed the corresponding experiment, namely the law of hot board causes little mouse\'s painful experiment of threshold of pain; Freun\'s complete adjuvant caused the swollen experiment of big mouse\'s foot;The xylene caused the swollen experiment of little mouse\'s ear; Invigorating blood circulation silt experiment that changes the sex that the rheology appearance examines the blood and flows with the blood; Useing the high performance liquid chromatography-electrochemistry Detected monoamine neurotransmitters transmitter in the brain and serum; the experiment of immunohistochemistry measured the proto-oncogene C-FOS expresses.

针对闭合性软组织损伤后的红、肿、热、痛及功能障碍的典型临床体征，加上无骨折、无皮肤破损的特点，我们设计了相应的实验，即热板法致小鼠疼痛的痛阈实验；福氏完全佐剂(Freun\'s complete adjuvant，FCA)致大鼠足肿实验；二甲苯致小鼠耳肿实验；用血流变仪测血液流变性的活血化瘀实验；运用高效液相色谱—电化学(high performance liquid chromatography-electrochemistry Detection HPLC-ECD)法测量大脑及血清中单胺类神经递质的实验以及免疫组织化学法检测原癌基因C-FOS蛋白产物表达的实验。

Immune POF model elicited by immunization in subcutaneous with antigen sourced isotype animal ovaries can cause growing follicles and mature follicles decrease obviously, the morphology of oocytes and zona pellucida abnormal, the adjacent granular cells disorder and atresic follicle increase.

以同种动物卵巢为抗原主动免疫建立的免疫性POF模型可致生长卵泡和成熟卵泡数明显减少，卵母细胞及透明带形态异常，周边颗粒细胞排列紊乱，闭锁卵泡增多，病理损伤随免疫时间延长而加重。

Dendritic cell vaccine was impacted and sensitized by tumor associated antigen and antigen polypeptide in vitro, then was retransfused or immunogenically inoculated to tumour carrier host, can evoked specific CTL's antitumour immunoreaction.

应用肿瘤相关抗原或抗原多肽体外冲击致敏DC制成疫苗，回输或免疫接种于载瘤宿主，可诱发特异性CTL的抗肿瘤免疫反应。

The dynamic changes of endothelin-1 in cerebrospinal fluid , brain tissues and plasma and ET〓-RNA expression in hypothalamus, hippocampus and temporoparietal grey and white matters were observed by radioimmunoassay methods and spot hybridization respectively.

四、建立双后肢高速、低速投射物致伤犬应激模型，应用核酸斑点杂交和放射免疫分析法，检测分析致伤前及致伤后10min、1h、2h、6h、8h，对犬的脑组织、血浆、脑脊液中的ET〓（内皮素-1）含量及海马、丘脑下部、颞顶叶灰、白质中ET〓-RNA表达的动态。

This leaves children highly sensitised to the particular allergen, which can lead to asthma attacks when their immune system reacts to any subsequent exposure.

这使得儿童对特殊致敏原高度敏感，当他们的免疫系统对以后接触到的任何致敏原起反应时都能诱发哮喘发作。

To elucidate the molecular basis of the attenuation ofDLA-EIAV in virulence thus provide theory foundation for designing HIV vaccine, thewhole genomes of DLA-EIAV and EJAV L provirus were cloned and sequenced. Aninfectious molecular clone derived from DLA-ELAV was constructed and characterized.Three DLA-EIAV/ L chimeric viruses were constructed. Promoting efficacy of the longterminal repeat of the DLA-EIAV was charactenzed.

为了揭示我国马传贫弱毒疫苗毒力致弱及免疫保护的分子机制，为人免疫缺陷病毒及其它慢病毒的免疫预防提供借鉴，我们对马传贫驴白细胞弱毒及其亲本马强毒EIAV L株前病毒进行了全基因克隆和序列测定，比较分析了二者的前病毒基因组核苷酸序列，在此基础上构建了EIAV驴白细胞弱毒株的感染性分子克隆和三株含有EIAV强/弱毒嵌合病毒基因组的重组质粒，并对EIAV弱毒株长末端重复序列的启动子活性进行了初步研究。

To elucidate the molecular basis of the attenuation ofDLA-EIAV in virulence thus provide theory foundation for designing HIV vaccine,thewhole genomes of DLA-EIAV and EIAV L provirus were cloned and sequenced.Aninfectious molecular clone derived from DLA-EIAV was constructed and characterized.Three DLA-EIAV/L chimeric viruses were constructed.Promoting efficacy of the longterminal repeatof the DLA-EIAV was characterized.

为了揭示我国马传贫弱毒疫苗毒力致弱及免疫保护的分子机制，为人免疫缺陷病毒及其它慢病毒的免疫预防提供借鉴，我们对马传贫驴白细胞弱毒及其亲本马强毒EIAV L株前病毒进行了全基因克隆和序列测定，比较分析了二者的前病毒基因组核苷酸序列，在此基础上构建了EIAV驴白细胞弱毒株的感染性分子克隆和三株含有EIAV强/弱毒嵌合病毒基因组的重组质粒，并对EIAV弱毒株长末端重复序列的启动子活性进行了初步研究。

Objective To observe the expression of connexin-32 and glial fibfillary acidic protein and their mutual relationship in forebrain of pentylenetetrazol induced seizure in rats ,after pretreatment with carhenoxolone.

目的研究大鼠存脑室埋管注射片珀酸预处理后戊四氮导致癫痫发作时前脑内胶质纤维酸性蛋白和连接蛋白-32（Cx32）表达及其相互关系：方法动物分为生理盐水对照组、CBX预处理对照组、戊四氮致痫组、CBX预处理后再PTZ致痫组，应用免疫荧光组织化学双重标记显示GFAP和Cx32在前脑的表达及其相互关系。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。