至

Objective To establish a HPLC method for simultaneous determination of oleanolic acid and ursolic acid in Erzhi pill and provide some bases for its quality control.

目的 建立高效液相色谱同时测定二至丸中齐墩果酸和熊果酸含量的方法，为二至丸的质量控制提供科学依据。

Then Vero cells were inoculated with a final cell density of 1×105/mL . After 7 days of culture, cell density can reach 6~7×106/mL, then rabies virus VaG strain was inoculated with a MOI of 0.01. After 24h inoculation, the cultured viruses were harvested continuously for 12 days. The titer of collected virus in the culture medium was about 6.0 to 8.5 logLD50/mL. From these collected viruses, vaccines were prepared through the steps of concentration, inactivation and purification, The quality of these vaccines meets "The Chinese Requirements for Biologics Year 2000".

在5L体积的生物反应器中，加入含10g/L微载体的199培养基，接种Vero细胞至细胞浓度达到1×105/mL，培养7d后细胞可生长至6~7×106/mL，然后以感染复数为0.01接种狂犬病毒VaG株，接毒后24h开始收获，连续收获12d左右，收获的病毒滴度范围在6.0~8.5logLD50/mL，收获的病毒原液经浓缩、灭活和纯化等步骤制备成疫苗，各项质量指标均达到《中国生物制品规程》2000年版要求。

The novel device integrates two important microfluidic phenomena, including hydrodynamic focusing and valveless flow switching inside multi-ported micro-channels. In this study, a simple theoretical model based on "flow-rate-ratio" method is first proposed to predict the performance of the device. Based on these data, a pre-focused 1xN flow switch is designed and fabricated using micromachining techniques.

本专题中的微流体晶片同时涵括了以下两种很重要的微流体现象--(1)水力预集中（hydrodynamic pre-focused ）以及(2)无阀式开关的切换方式，并在同一平面上作流向控制，以便将相同样品注射至不同或相同之出口槽(output-port)以连接至不同的微流体分析晶片，以达成多功能连续式的样品进料功能。

Spikelets lanceolate to narrowly oblong, dorsally compressed; callus short, bearded, hairs usually less than 1/2 spikelet length; glumes cartilaginous to leathery; lower glume flat or slightly concave, hairy, back veinless or inconspicuously veined, flanks rounded in lower half, keeled toward apex, acute to truncate; upper glume boat-shaped, 1-keeled down midline; lower floret reduced to an empty hyaline lemma, rarely absent; upper lemma linear to oblong, occasionally broader, shortly 2-toothed or 2-lobed to middle, awned E.

小穗披针形的到狭长圆形，背压扁；胼胝体短，具髯毛，不到1/2的小穗长度的通常毛；颖片软骨质的到革质；更下部的颖片平或稍凹，有毛，背无脉或不明显，侧面圆形在下半部，龙骨状向先端，锐尖到截形；舟状的上面颖片，沿着midline龙骨状的1；更低的小花退化至一空的透明的外稃，很少无；上面外稃线形到长圆形，偶有宽，2齿或2裂的至中部，具芒的E。

Objective To observe the clinical effect in treating metastatic liver cancer in artery interventional and vein chemotherapy with oxaliplatin, 5-fluorouracil deoxyribonucleoside and citrovorum factor, mitomycin +lipiodol.Methods 38 non-operable cases of metastatic liver cancer were given transfemoral artery chemoembolization, catheterical head was placed into tumors feeding artery(common hepatic artery, left of right hepatic artery, hepatic segmental artery), veinous chemotherapy in the next day.

目的 观察奥沙利铂(Oxaliplatin， L-OHP)联合氟尿嘧啶脱氧核苷、亚叶酸钙、丝裂霉素+超液化碘油动脉介入+静脉化疗双通路对转移性肝癌的临床疗效方法不能手术的转移性肝癌38例，经皮股动脉穿刺插管至腹腔动脉，并超选至肿瘤的供血供氧血管后灌注化疗药并栓塞，于次日再给予静脉化疗。

Results HWTL and HWCL decreased significantly from day 1 to day 14 after TO injection with the lowest value on day 5 and day 3, respectively, and both recovered on day 21. The number of TRPVl-labeled neurons increased remarkably from day 1 to day 14 with a peak on day 7, and returned back to the normal level on day 21. In control rats, only small and medium-sized TG neurons were immunoreactive to TRPV1,and the TRPV1-IR terminals were abundant in both the vibrissal pad and the Vc.

结果 注射松节油后第1至14天，热退缩反应潜伏期与冷退缩反应潜伏期均明显下降，分别于注射后第5天和第3天达到最低，第21天恢复到正常水平；注射松节油后第1至14天，TRPV1表达的细胞数量增加，并于第7天达到最大，第21天恢复到正常水平。

Results HWTL and HWCL decreased significantly from day 1 to day 14 after TO injection with the lowest value on day 5 and day 3, respectively, and both recovered on day 21. The number of TRPV1-labeled neurons increased remarkably from day 1 to day 14 with a peak on day 7, and returned back to the normal level on day 21. In control rats, only small and medium-sized TG neurons were immunoreactive to TRPV1, and the TRPV1-IR terminals were abundant in both the vibrissal pad and the Vc.

结果 注射松节油后第1至14天，热退缩反应潜伏期与冷退缩反应潜伏期均明显下降，分别于注射后第5 天和第3 天达到最低，第21 天恢复到正常水平；注射松节油后第1至14天，TRPV1表达的细胞数量增加，并于第7天达到最大，第21 天恢复到正常水平。

The intensity ratio of TO and LO inMCT was observed to be different. Such difference was explained in terms of the different Ramangeometry arrangement.〓. The laser-induced micro-photoluminescence in the range of 1000～5000〓(1.34eV～1.83eV) was found for the first time in LPE MCT epilayer. The center of photoluminescence wasat 2750〓 or 1.62eV and the FWHM of luminescence was 2000〓 or 0.25eV. We assume thatthe photoluminescence is due to recombination of electron from an anion vacancy resonance levelto the top of valance. In addition, new Raman shift was observed at 750〓 in LPE MCTepitaxial film.〓. The laser-induced micro-photoluminescence with quasi-periodic structure was observed forthe first time at room temperature in one of MOVPE MCT epitaxial film samples. The range offluorescence was from 1.46eV to 2.21eV, i.e., 1.73eV above the conduction band edge.

2首次在LPE生长的碲镉汞外延薄膜的显微Raman谱中，在1000～5000〓范围发现了激光激发显微荧光，该荧光的发光范围换算为电子伏特标度为1.34eV～1.83eV，荧光的发光中心大约位于2750〓，即1.62eV，发光的半峰高宽约为2000〓或0.25eV；指出该显微荧光来源于碲镉汞薄膜中的阴性离子空位共振能级的激光激发发光；观察到了碲镉汞外延薄膜中一个新的Raman散射峰，位于750〓位置； 3首次在一块用MOVPE方法生长的〓Te外延薄膜的显微Raman谱中，发现了1.46eV至2.21eV范围并伴随有周期结构的显微荧光峰，该发光峰对应的能带中心位于〓Te材料导带底上方1.73eV，通过研究得出样品在1.46eV至2.21eV范围的显微荧光峰是由于改进 MOCVD 生长工艺，提高了碲镉汞外延薄膜的结构质量所致；通过分析指出该显微荧光来源于外延层中的阴性离子空位的共振能级发光。

My worktime/hours are Monday to Friday, from

我的工作时间为周一至周五，从早上六点半至下午三点半。

Consequently the financial structure of listed Chinese companies is changing quickly: net debt rose from 23 per cent of equity in 2002 to 36 per cent in 2006 and from 1.1 times earnings before interest, tax, depreciation and amortisation to 1.3 over the same period.

因此，中国上市公司的财务结构正在迅速变化：净债务在总股本中所占比率，从2002年的23%，升至2006年的36%，同时对未计利息、税项、折旧和摊销前收益的倍数，从1.1倍升至1.3倍。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。