至

We selected the 2nd and the 3rd generation yeast with powerful vitality from the yeast mud fermented in tanks in working field as the ideal species source separated from yeast monoplast in beer and the ways to obtain them were as follows: 24 hours after deoxidisation of diacetyl to threshold value and decrease of its temperature to 5℃ was the best time for selection and sample－extraction location was at the nozzle of pressure－discharge pipes.

摘 要：从生产现场大罐发酵的酵母泥中选取第2～3代生命力强的酵母作为啤酒酵母单细胞分离的理想种源，选取的最好时间为双乙酰还原至阈值后降温至5℃，并让其停留24h时取样位置为排压酵母的管口，最后将所得菌种进行初选、复选或模拟生产恒温培养鉴定，选出优良菌株。

METHODS: Rat bilateral olfactory bulbs and olfactory mucosa at 1/3 nasal septum were obtained, sliced, digested in trypsin, and made into monoplast suspension. At 1×109/L, cells were incubated in uncoated 25 cm2 culture flask. At 18-20 hours, cell suspension was moved into another uncoated 25 cm2 culture flask (the first differential adhesion). At 24 hours, cell suspension was moved into a poly-D-lysine-coated 25 cm2 culture flask or poly-D-lysine-coated 6-well culture plate (the second differential adhesion). At 48 hours, parenchyma cells were removed after a half of medium was changed.

完整取大鼠双侧嗅球及剪取鼻中隔后1/3嗅黏膜，剪碎后胰酶消化，制成单细胞悬液，按1×109 L-1密度接种于未包被的25 cm2玻璃培养瓶中，18~20 h后将细胞悬液转移至另一未包被的25 cm 2玻璃培养瓶中（第1次差速贴壁），24 h后再将细胞悬液转移至经多聚右旋赖氨酸包被的25 cm2塑料培养瓶或经多聚右旋赖氨酸包被的6孔培养板中（第2次差速贴壁），接种培养48 h后半量换液以去除杂细胞。

When reached logarithm growth phase, it was poured or the medium was removed. 5 mL medium containing 10 mg/L mitocin-C was added , kept at 37 ℃, and then cultured in saturated humidity warm box with the CO2 of 0.05 fraction volume for 2 or 3 hours. It was coated with 0.1% gelatin in 6-hole plate, laid for over 30 minutes, and then threw away or the medium with mitocin-C was removed, washed with phosptat buffer for 5 times so as to remove the mitocin-C. 2 mL 0.05% trypsin digestive cells were added, and it was observed under microscope. When crevice appeared, cells became round (about 2－4 minutes), digestion was stopped by adding medium of the same volume, and blew up with straws repetitively to make it into monoplast suspension. Special-used cover glass was put in the center of cell counting chamber. Cells were sucked in by glass siphon, and cell suspension flew out at bucket of up or down-sides of counting chamber, until the cover glass was filled with fluid. Living cell were inoculated at concentrations of 3×108, 5×108, 1×109 L－1 after their number counted.

选取2～5代的小鼠胚胎成纤维细胞，待其至对数生长期倒掉或吸掉培养基，加入含10 mg/L丝裂霉素C的细胞全培养液5 mL，置37 ℃，体积分数0.05的CO2饱和湿度温箱中培养二三小时，在6孔板中加入0.1%明胶包被，放置30 min以上，倒掉或吸掉含丝裂霉素C的培养基，磷酸盐缓冲液清洗5遍，尽量除去丝裂霉素C，加入2 mL 0.05%的胰蛋白酶消化细胞，镜下观察，当细胞间出现裂隙，细胞变圆时（约2～4 min），立即加入等量的全培养液终止消化，并用吸管反复吹打，使之成为单细胞悬液，在细胞计数板中央放置专用的盖玻片，用玻璃虹吸管吸取细胞，让虹吸管在盖玻片上或下侧的计数板凹槽处流出悬液，至盖玻片下被液体充满为止。

The preliminary results showed that the extracellular domain of YWK- II protein was able to interact with the 303-376 aminoacids segment of the rat Mullerian inhibiting substance in a yeast two-hybridsystem.

我们利用分子生物学的方法对YWK-Ⅱ蛋白质的463位至674氨基酸及389位至574位氨基酸的两段肽段分别进行了表达、纯化、结晶和初步解析了其中一段胞外区的结构，并且得到了YWK-Ⅱ和MIS的复合物的晶体。

It is demonstrated that at the temperature of 40°C, the Sb_2O_3 nanorods with different lengths (2~50μm) and diameters (20~1000 nm) would axially self-assemble into multisegmented coaxial nanowires by an oriented attachment assisted end-to-end self-assembly process.

研究表明，在40摄氏度下，不同长度的(2至50微米)和直径(20至000纳米)的三氧化二锑纳米棒，通过&头碰头&的&定向接触&的自组装过程，沿轴向自组装成三氧化二锑多段同轴纳米线。

The myelinization of cerebellar white matter,middle cerebellar peduncles,anterior limb of internal capsule,splenium and genu of corpus callosum,centrum semiovale,the white matter in parietal-occipital area and frontal-temporal area were documented at birth to 5,2～4,3～7,3～5,4～7,2～7,3～9 and 4～12 months old,respectively.

髓鞘形成至成熟时间：小脑上下脚、桥脑背侧、丘脑腹外侧和内囊后肢后部出生时已成熟，小脑自出生至5个月，小脑中脚2～4个月，内囊前肢3～7个月，胼胝体后部、膝部分别是3～5个月、4～7个月，半卵圆中心2～7个月，枕顶叶白质3～9个月，额颞叶白质4～12个月。

The following delighting results were observed: in general, Grandma could take care of herself, her verbal ability greatly improved and made effective communication possible though some individual words were indistinct, the myodynamia of her right arm was enhanced to level 4-5, and that of her right leg to level 5, her blood pressure returned to the normal values, 138/80mmHg, and she could independently perform the daily activities such as taking a shower, getting dressed, and eating meals.

至患者病后第20天止，患者生活自理能力已基本恢复，语言能力也得到了大幅度改善，除个别字词说不清之外，其余已无大碍，右上肢肌力恢复至4-5级，右下肢肌力5级。血压回复到正常范围内138/80mmHg ，洗澡、穿衣、吃饭等日常琐事已可自行打理，无需他人帮忙。

Fifteen novel 1,4-naphthoquinones, rhinacanthin-A -D, rhinacanone-A -K and two new biphenyl compounds, rhinacanim-A,-B together with eleven known compounds have been isolated and characterized from the root of Rhinacanthus nasutus Kurz.

从白鹤灵芝草的根部甲醇抽取物中经过各种层析分离纯化后共得到15种新的1，4-naphthoquinone类化合物，分别命名为Rhinacanthin-A至-D，Rhinacanone-A至-K及二种新的Biphenyl化合物，Rhinacanim-A，-B和11种已知化合物。

Fifteen novel 1,4-naphthoquinones, rhinacanthin-A -D, rhinacanone-A -K and two new biphenyl compounds, rhinacanim-A,-B together with eleven known compounds have been isolated and characterized from the root of Rhinacanthus nasutus Kurz.

中文摘要从白鹤灵芝草的根部甲醇抽取物中经过各种层析分离纯化后共得到15种新的1，4-naphthoquinone类化合物，分别命名为Rhinacanthin-A至-D，Rhinacanone-A至-K及二种新的Biphenyl化合物，Rhinacanim-A，-B和11种已知化合物。

The December issue reports that stage 3 to 5 CKD is found in 4% to 5% of the adult population in the United Kingdom, most often in elderly individuals who have comorbidities for which they are prescribed several drugs. The authors remind practitioners that patients with impaired renal function have decreased drug clearance by the kidney and may be more susceptible to certain nephrotoxic drugs. They advise:"Whenever possible, should take steps to minimize these risks, by avoiding the drugs, using reduced doses or administrative frequency, or increasing the monitoring of renal function" by measuring estimated glomerular filtration rate.

12月号的报导刊载了英国人口中，有4%至5%是CKD第3至5级，大部分是老年人，且这些病患经常使用多种药物；作者提醒执业医疗人员，肾脏功能不全病患其肾脏排除药物的清除率下降，而且更可能受到某些具有肾毒性药物的伤害；他们建议，当情况允许时，执业人员应该将这些风险降到最低，透过避免使用部分药物，或是降低剂量、或频次，或是增加监测肾丝球廓清率的频率来评估肾脏功能。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。