自然法

Five models were used to investigate the "Changdong"" s anti-tumor effect: mice S180 sarcoma solid model, mice liver cancer H22 solid model, mice Lewis lung cancer model, as well as human liver cancer QGY and colon cancer LOVO implanted in naked mice; mice liver cancer H22 was also used to evaluate the enhancive effect of "Changdong" when it was co-applied with other anti-tumor agent or with radiotherapy; The "Changdong"" s cytotoxic effect in vitro on such tumors as QGY, LOVO, lung cancer A549, breast cancer MCF-7, as well as mice leukemia P388 was observed through MTT method. And investigate the effect of "Changdong" on proliferation of spleen lymphocyte, on activity of NK cell and on synthesis of IL-2 in mice bearing Lewis lung cancer, respectively. With propidium iodide straining, cell aigptosis and cell circle were analyzed by flow cytometry. Result:"Changdong" has an evident tumor inhibitive effect on mice tumor model as well as human tumor implanted in naked mice with a quantity-effect relationship.

使用小鼠S180肉瘤、小鼠肝癌H22实体瘤和小鼠Lewis肺癌模型，以及人体肝癌QGY和人体肠癌LOVO裸鼠异种移植模型，进行"长动"的抗肿瘤药效学研究：用小鼠肝癌H22进行"长动"合并放疗和化疗的增效作用研究；用MTT法进行"长动"对肝癌QGY、肠癌LOVO、肺癌A549、乳腺癌MCF-7、小鼠白血病P388等10株人体和动物肿瘤细胞的体外细胞毒性作用研究；用淋巴细胞转化试验、小鼠NK细胞活性试验、小鼠胸腺细胞增殖法分别测定"长动"对荷Lewis肺癌小鼠的脾淋巴细胞增殖的影响、自然杀伤细胞活性的影响和对小鼠产生白介素-2(IL-2)的影响；用流式细胞仪PI单染色法进行"长动"对人体肝癌QGY细胞的体外诱导凋亡作用和对肿瘤细胞周期的影响的研究。

Based on the eco- environment function, confirmed the constitution of eco- environment water demand(including the inside of the watercourse.and the ecological water demand of the outside of the watercourse such as ecological water demand of economical crop, of lake or reservoir and of cities.).③Analyzing the physical geography, hydrography, economy characteristic and water resource status, getting the proper calculation method. The Tennant applied to the eco- environment water demand of inside of the watercourse, woodland minmum eco- environment water demand calculation method applied to the eco- environment water demand of woodland, water ration method applied to farmland the eco- environment water demand, water evaporation method applied to the reservoir eco- environment water demand.

本文以流域生态环境需水为主要研究对象，因此重点阐述了流域生态环境需水量的分类和计算方法；②通过分析流域生态系统构成与流域生态环境功能，并分时段和河段考虑，确定了桃花江流域生态环境需水量的组成，即包括维持现状河道生态环境功能现状的现状生态环境需水量与流域生态环境改善的生态环境需水量，前者包括河道内生态环境需水量(维持河道基本生态功能需水量、维持河道自净稀释功能需水量、补充地下水水量及美化流域景观娱乐需水)与河道外生态环境需水量(流域内植被、森林、农田的需水量和与河道相连的池塘、水库等的需水量)；后者还包括为治理改善流域生态环境而拟规划林业生态建设所需水量；③对桃花江流域的自然地理、水文地质和社会经济特征以及水资源现状及其主要生态环境问题进行了综合分析，提出了适合桃花江流域的生态环境需水量计算方法，即采用Tennant法计算河道内生态环境需水量，采用林地最小生态环境需水量法计算林地生态环境需水量，采用需水定额法计算农田生态环境需水量，采用水面蒸发法计算水库、池塘生态环境需水量。

In this paper, three methods for determining the natural background of Υ-radiometers are studied: the methods of water-surface,lead-screen and submersion.

本文讨论了三种测定γ辐射仪自然底数的方法：水面法、铅屏法和水中法。

To compare the temperature diversity by collecting and analysing these infrared thermogram data. Results: While the operator just put their hands to the back of the quizzee for 3 minutes, the temperature of Xinshu and Dazhu rised and then descent gradually; But the temperature rised all the same about another 3 minutes while the operater using the vibrating manipulation; The temperature of Laogong and Zhongchong rised gradually, especially in 120 seconds and 90 seconds after using the vibrating manipulation.

结果：三组操作者在空置后3min，心俞穴和大杼穴处温度均出现变化，且与操作前温度有显著差别(P.05)；在空置3min后，被操作者的心俞处以及大杼处的温度呈自然下降趋势；而在行掌振法时，被操作者心俞处以及大杼处的温度变化，不仅发生在3min后，而且可延续到振法停止后的1min到3min内，与操作前温度相比，有显著差别(P.05)；在空振中，高年资医师的劳宫处和中冲处温度呈现逐渐上升趋势，在振法进行到90s时，中冲处温度出现显著变化，与开始温度相比，有显著差别(P.05)，而劳宫处的温度则是在120s时，出现显著变化，与开始温度相比，有显著差别(P.05)。

objective to investigate law of development of chrysomyia megacephala in natural condition,and to probe its application in forensic medicine.methods rate of development and total effective temperature of chrysomyia megacephala were observed,recording the result and statistical analysis.results two equation of linear regression（y1=0.430 8+0.237 1x,y2=75.684 6-1.444 7x） were established between the total effective temperature and the length or weight of chrysomyia megacephala.conclusion it can be used to estimate the time of death in forensic medicine practice depending on the mathematical model from the effective temperature.

目的 研究大头金蝇在自然条件下的发育规律，探讨其在法医学中的应用。方法对大头金蝇在自然条件下的发育速度及有效积温进行观察、记录和统计学处理。结果确立了y1=0.430 8+0.237 1x，y2=75.684 6-1.444 7x 2个有效积温与蛆长和蛹体质量的线性回归方程。结论在自然条件下用积温法建立起的大头金蝇发育数学模型可应用在法医学死亡时间推断中。

Methods Freshmen and junior of medicos were selected,and parasitological examination were conducted for the infections of intestinal parasite,in-cluding physiological saline direct smear method,saturated brine flotation,modified Kato's thick smear and natural sedimentation.

目的 了解某高校医学生肠道寄生虫感染状况，在校学生寄生虫防治工作提供依据方法采用生理盐水直接涂片法、饱和盐水漂浮法、厚涂片透明法、自然沉淀法对某高校、三年级大学生659人进行调查。

Methods Freshmen and junior of medicos were selected,and parasitological examination were conducted for the infections of intestinal parasite,in-cluding physiological saline direct smear method,saturated brine flotation,modified Kato's thick smear and natural sedimentation.

采用生理盐水直接涂片法、饱和盐水漂浮法、厚涂片透明法、自然沉淀法对某高校一、三年级大学生659人进行调查。

The advantages of the simple twice zymotechnics lie in the characters of the cultivation materials, the growth of mycelia, the control of plant diseases and insect pests, the output of Volvariella Volvacea and the production cost. All these indicate that simple twice zymotechnics is a relatively reasonable compost treatment in Volvariella Volvacea cultivation.

简易二次发酵法的栽培材料理化性状、草菇菌丝生长、病虫害控制和草菇的产量、生产成本等，都比自然堆沤发酵法和常规二次发酵法有较大的优势，表明简易二次发酵法是草菇栽培中较理想的一种培养基处理方法。

With conventional PCR primer pair XBFF/R,the same 352bp bands appeared by amplifying all the DNA samples from the eight races,and no bands appeared by amplifying all the DNA samples from the 9 plant pathogen species(Cucumber powdery mildew,wheat yellow rust,wheat leaf rust,wheat stem rust,wheat scab,wheat sheath blight,wheat loose smut,maize sheath blight and maize head smut)and control.

3用常规PCR和实时荧光PCR两种方法对温室接种的小麦白粉病进行了检测，并在时间上与自然显症的观察进行了比较，结果表明：在温室条件下，小麦白粉病菌在接种后第5d时开始出现小麦白粉病病斑，而利用常规PCR引物XBFF/R可在接种第3d检测到小麦白粉病菌，比自然显症观察能够提前了2d检测到白粉病菌；实时荧光PCR法可在接种后1d即能检测到小麦白粉病菌，比自然显症观察能够提前了4d检测到白粉病菌，这可为白粉菌的预测或预警检测提供依据，更可为白粉病在流行前制定防治决策赢得宝贵时间。4。

Electric methods include resistivity profiling, resistivity sounding,"mise-a-la-masse" method, induced polarization method and self-potential method.

在每一大类中，又有一系列的方法，例如直流电法中就包括电阻率剖面法，电阻率测深，充电法，激发极化法和自然电位法等

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。