自流

The results show that the duration of the 3 phases during the culture of cell suspension is related to the inoculum size, the initial nutrient concentration, the feeding of nutrients and the culture conditions such as temperature and dissolved oxygen, that the maximum cell yield reaches about 12g/L for batch culture or 35g/L for fed-batch culture in dry weight, that the saponin content of cells quickly increases in the bloom phase and keeps on increasing until the early stage of the declining phase, with a maximum saponin content of about 60~70g/kg in dry weight, that the conductance value of cell suspension decreases from more than 8000μS to less than 600μS with the growth of cells, but increases when the cells autolyze, and that part of cells turns red in the later culture, which has no apparent effect on the saponin content.

结果显示：细胞3个生长时期的持续时间与接种量、初始营养浓度、营养流加情况及培养条件有关；分批培养和流加培养时的最大细胞得率分别为12g/L和35g/L；细胞的皂甙含量在旺盛生长期快速上升，并保持增长至衰退期前期；干细胞中最大的皂甙含量达60~70g/kg；随着细胞的生长，培养液的电导值从8000μS以上下降至600μS以下，细胞自溶后逐渐回升。另外，培养后期部分细胞颜色变红，但没有发现颜色转变对皂甙含量造成影响。

This work was first brought forward in SVC,and it can not only enhance error resilient capability of SVC bit-stream,but also maintain consistent bit-rates between input and output of the transcoder.4 This dissertation proposes discardable data adaptation in encoder.

该方法在SVC中首次提出，不仅能提高码流的容错能力，且能保证经过转码器的码流输入输出比特率的一致性。4提出SVC编码端的可丢数据自适应技术。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

We show that the twoBEC system may reach a selftrapping stationary state through adjusting interatomic interactions and the initial state of the system under our consideration.

我们表明在一定条件下此系统可达到一个自囚禁的稳态。我们还研究了两个凝系间的隧穿原子流，发现在一定条件下可获得一个只含纯直流的原子流。

Hence the nonconservation parts of the spin and orbit currents can not counteract each other precisely.

因此， 普遍定义下的自旋流密度和轨道流密度的不守恒部分并不能相互抵消。

In this paper, the stress and strain fields of lithosphere in Himalayan Western Syntaxis orogenic belt and its adjacent areas since 10Ma are investigated through using the 3-D finite-element simulation, and deformation and evolution process in former 2Ma are simulated with different rheological parameters and rheological structure. Studies on functions in simulation process of different viscosity parameters, constitutive relation and changes of initial curst thickness show below results:(1) When various viscoelastic models are adopted, the selection of the coefficient of viscosity in the Western Syntaxis area has important influence on the its uplifts and evolutions. The computational results show that the viscosity in the Western Syntaxis area should be smaller than 1023Pa·s;(2) When the elastic model is adopted in relatively rigid Tarim basin, obvious changes are induced to the stress and strain fields of the whole Western Syntaxis area;(3) The existence of mechanical property stable block doesn′t make the propagation of the stress field show obvious decoupling phenomenon.

用数值模拟的方法探讨了自10 Ma以来喜马拉雅弧形造山带的西构造结及邻区岩石圈的形变和位移变化特征，并采用不同的流变参数和流变结构模拟了前2 Ma内的形变和应力场的演化过程，通过对不同黏性系数、本构关系和初始地壳厚度的改变在演化过程中所起的作用研究表明：（1）采用黏弹性模型时，西构造结区黏性参数的选取对西构造结及邻区隆升高度和隆升范围有重要影响，计算结果给出西构造结区地壳黏性系数应小于1023Pa·s；（2）相对刚性的塔里木盆地采用弹性结构时，对整个西构造结区域的应力和应变场均能引致明显变化；（3）力学性质稳定块体的存在并没有使应力场的传播出现明显的解耦现象。

Poposed a two-time-scale rate-control algorithm for layered video transportation. Self-similarity is prevalent for variable kind of network traffic, and the traditional flow control algorithms are recognized as unappropriate for such traffic.

提出了支持分层编码视频流传输的双时间尺度速率控制策略网络聚集数据流的自相似特性使得在对数据流进行流量控制时不能采用过去的基于单一时间尺度的速率控制算法。

For the word of Hu Weiwu of total stylist of the core that use dragon, do a technology to need to start protracted war in certain moment, the idea early days that I design to working shedding system is do to satisfy the requirement of the market and client, and now is the research that plans to do endurance and development The friend that has industry the other day is flowing carefully in the problem that the trends in moving process revises about the job, say this demand is at present more, whether to know to get used to a design to you can satisfy this kind of requirement oneself?

用龙芯总设计师胡伟武的话来说，搞技术在某些时候需要搞持久战，我对工作流系统设计的想法前期是为满足市场和客户的需求而做，而现在是打算搞持久性的研究和开发前些天有业界的朋友在仔细关于工作流在运行过程中动态修改的问题，说目前这个需求比较多，不知道自适应设计是否能够满足这种需求？

The LBL self-assembly process was monitored by flow cytometry and zeta potential analyzer. Scanning electron microscope, dynamic light scattering, and flow cytometry were used to characterize microcapsules The microcapsules were 1.14 μm in size and stable in lyophilizing.

在微囊的制备过程中，采用流式细胞仪和Zeta电位仪对层层自组装进行监测，并对制得的微囊采用扫描电子显微镜、动态光散射仪、流式细胞仪等进行表征，结果发现微囊的直径在1微米左右，尺寸较均一。

Methods: Porcine livers were harvested from common health dogs, performed by dual vessel perfusion and oxygenating blood together.

自普通健康的杂种犬获取供肝，采取同时灌流门静脉和肝动脉，共同氧合灌流液的方法对离体肝脏进行灌注试验。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！