自体移植的

Two months after surgery, tuftsin and IgM levels had decreased dramatically in the control group but remained stable in the splenic autotransplantation group.

术后两个月，血清tuftsin和IgM水平在对照组出现显著地下降，而在接受脾脏自体移植的患者中仍维持稳定。

Stem cells in bone marrow are considered as an ideal sources of seed cells with the advantage of being isolation and easy proliferated in vitro, which is a prerequisite for the autotransplantation.

存在于骨髓内的干细胞来源丰富，采集方法简单，体外扩增容易，具有自体移植的必要条件，是一种理想的种子细胞。

BACKGROUND: Extract of astragalus mongholicus has good effects on antioxidization and oxygen free radical scavenge. Bone marrow mesenchymal stem cells have multiple directional differentiation potential and superiority of autologous transplantation. This can provide a new way for treating neurodegenerative diseases.

背景：黄芪提取物有较好的抗氧化、清除氧自由基的作用，加之骨髓间充质干细胞的多向分化潜能及其自体移植的优越性，可能为神经退行性疾病的治疗提供新的方式。

Seven patients aged 4 to 19 years, with myelomeningocele and high-pressure or poorly compliant bladders, were identified as candidates for cystoplasty. Each patient underwent bladder biopsy, and urothelial and muscle cells were grown in culture and seeded on a biodegradable bladder-shaped scaffold made of collagen, or on a composite of collagen and polyglycolic acid. About 7 weeks later, the autologous engineered bladder constructs were used for reconstruction, and implanted either with or without an omental wrap. After implantation, patients underwent serial urodynamics, cystograms, ultrasounds, bladder biopsies, and serum analyses.

年纪从4到19岁的7位脊髓脊膜膨出、罹有高压或者功能不良膀胱、被鉴定需进行膀胱成形术的病患，每一位病患接受膀胱切片，将上皮与肌肉细胞在膀胱形状的生物可分解的胶原支架或胶原聚羟基乙酸支架上加以培养；大约7周过后，形成一可自体移植的工程膀胱，移植时包覆或者未包覆网膜；移植后，病患进行一系列的尿路动力学、膀胱造影术、超音波、膀胱切片和血清分析。

In order to get enough genetically modified cells for autografting, the effects of bFGF and EGF on the muscle satellite cells in primary culture from adult humans aged over 40 were observed in the present study.

本研究以获取基因治疗自体移植的载体为目的，观察了生长因子对成人骨骼肌卫星细胞增殖的影响。

Immunofluorescent method was employed to observe the development of autologous peripheral blood stem cells in the liver.

通过免疫荧光检测自体移植的外周血干细胞在肝脏内的发育情况。

To pay attention to carry out effective cardiopulmonary resuscitation, hemostasis by compression and deep vein intubation rapidly in the initial stage of emergency. The carotid shunt tube plays an important role in severe carotid injury and re-establishment process. We use external jugular vein to transplant and re-establish,which are rung with artificial blood vessels for the long distance carotid injury and achieved the best result. We expose as fully as possible for the hepatic venae and the posthepatic post caval injury,and adopt effective methods to stop bleeding and repair. The application of blood shunt pump could raise the success rate for those severe patients. The self-double vastransplantation with both ends being all trousers-style one opening solve some problems such as there are no suitable caliber self-transplantation blood vessels and the artificial blood vessels are easy to occur obliteration for long-term and so on for the great or middle blood vessels.We use improved Fogarty duct and anterograde intubation to eliminate thrombus could eliminate thrombus effectively and avoid accessory injury for the deep vein injury and extensive thrombosis.

抢救初期要注意进行有效的心肺复苏、压迫止血和快速进行深静脉插管；颈动脉转流管在严重颈动脉损伤重建过程中有重要作用；对长距离颈动脉损伤用颈外静脉移植外环包人造血管重建，可取得最佳效果；对肝静脉及肝后下腔静脉损伤，要充分暴露，采用有效方法止血和修复，对病情极危重的凶险性出血者，血液转流泵的应用可提高成功率；对大、中血管损伤，两端均为裤式单开口自体双血管移植，解决了无适宜口径自体移植血管和应用人造血管远期易闭塞等问题；对深静脉损伤并广泛血栓形成者，应用改进的Fogarty导管和顺行插管取栓法，可有效取栓并避免了副损伤。

To pay attention to carry out effective cardiopulmonary resus-citation,hemostasis by compression and deep vein intubation rapidly in the initial stage of emergency.The carotid shunt tube plays an important role in severe carotid injury and re-establishment process.We use external jugular vein to transplant and re-establish,which are rungwith artificial blood vessels for the long distance carotid injury and achieved the best result.We expose as fully as possible for the hepatic venae and the posthepatic post caval injury,and adopt effective methods to stop bleeding and repair.The application of blood shunt pump could raise the success rate for those severe patients.The self-double vastransplantation with both ends being all trousers-style one opening solve some problems such as there are no suitable caliber self-transplantation blood vessels and the artificial blood vessels are easy to occur obliteration for long-term and so on for the great or middle blood vessels.We use improved Fogarty duct and anterograde intubation to eliminate thrombus could eliminate thrombus effectively and avoid accessory injury for the deep vein injury and extensive thrombosis.

抢救初期要注意进行有效的心肺复苏、压迫止血和快速进行深静脉插管；颈动脉转流管在严重颈动脉损伤重建过程中有重要作用；对长距离颈动脉损伤用颈外静脉移植外环包人造血管重建，可取得最佳效果；对肝静脉及肝后下腔静脉损伤，要充分暴露，采用有效方法止血和修复，对病情极危重的凶险性出血者，血液转流泵的应用可提高成功率；对大、中血管损伤，两端均为裤式单开口自体双血管移植，解决了无适宜口径自体移植血管和应用人造血管远期易闭塞等问题；对深静脉损伤并广泛血栓形成者，应用改进的Fogarty导管和顺行插管取栓法，可有效取栓并避免了副损伤。

Three weeks afer the allografting,there was no significant difference between the two al lografting groups in maximum load(P＞0.05),Eight weeks later,the maximum load of the group that was preserved by liquid nitrogen after program freezing(76.60±4.67)N was better than the group that was preserved by －80℃ deepfrozen(75.99±4.20) and similar with the autografting group(76.72±2.55).4.The healing process and histological behavior of the two deepfrozen groups are similar with the autografting group,and the program freezing group is better.

结果 a经程序冷冻液氮保存方法处理后，髌腱的最大载荷无明显下降，细胞活性得到了较好的保存，组织学观察冷冻损伤较－80℃深低温保存方法轻微；b程序冷冻液氮保存处理的移植物在术后未表现明显的排斥反应，且免疫反应随时间的推移而下降；c移植后3周，各组移植物的最大载荷无显著差异(P＞0.05)，移植后8周，程序冷冻液氮保存组移植物的最大载荷(55.87±1.86)N优于－80℃深低温保存组(52.14±2.79)，而和自体移植组相近(57.70±2.76)N；d从组织学观察看，－80℃深低温保存组和程序冷冻液氮保存组移植后的愈合过程均和自体移植组相似，而程序冷冻液氮保存组的愈合过程和组织学行为更接近于自体移植组。

Results 1.After treated by:program freezing,the maximum load of the allografts has no evident decrease and the cell activity of allografts was preserved well,the frozen hurt after program freezing was more slight than －80℃ deepfrozen,2.No evident reject reaction can be seen during the healing process after deep frozen and the reject reaction declined as the time pass by.3.Three weeks afer the allografting,there was no significant difference between the two al lografting groups in maximum load(P＞0.05),Eight weeks later,the maximum load of the group that was preserved by liquid nitrogen after program freezing(76.60±4.67)N was better than the group that was preserved by －80℃ deepfrozen(75.99±4.20) and similar with the autografting group(76.72±2.55).4.The healing process and histological behavior of the two deepfrozen groups are similar with the autografting group,and the program freezing group is better.

结果 a经程序冷冻液氮保存方法处理后，髌腱的最大载荷无明显下降，细胞活性得到了较好的保存，组织学观察冷冻损伤较－80℃深低温保存方法轻微；b程序冷冻液氮保存处理的移植物在术后未表现明显的排斥反应，且免疫反应随时间的推移而下降；c移植后3周，各组移植物的最大载荷无显著差异(P＞0.05)，移植后8周，程序冷冻液氮保存组移植物的最大载荷(55.87±1.86)N优于－80℃深低温保存组(52.14±2.79)，而和自体移植组相近(57.70±2.76)N；d从组织学观察看，－80℃深低温保存组和程序冷冻液氮保存组移植后的愈合过程均和自体移植组相似，而程序冷冻液氮保存组的愈合过程和组织学行为更接近于自体移植组。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。