自体移植

The patients achieved complete remission after induction course would enter the next grouping:the patients,aged 15-45 years, who had suitable donors and transplantation will were assigned to allogeneic stem-cell transplantation,and for the rest,they would be assigned to accept chemotherapy or autologous stem-cell transplantation randomly.CT and ASCT group both received early intensification and consolidation therapy orderly,which contained high-dose methotrexate,intermediate/high-dose cytarabine and high-dose or high-fraction cyclophosphamide,followed by ASCT or re-induction,later consolidation and maintenance chemotherapy for CT group.

符合入组条件的成人非Ph+ALL患者纳入研究，患者接受标准VDCP±L诱导治疗达到完全缓解(complete remission，CR)后进入下一阶段分组研究：年龄≤45岁，有合适供者及移植意愿者接受异基因造血干细胞移植allogeneic stem cell transplantation，Allo-SCT其余患者根据意愿分别接受自体造血干细胞移植(autologous stem cell transplantation，ASCT和化疗Chemotherapy。

An area of (1.5×1.5) cm2 autologous full thickness skin graft model was made on the back of the all the rats. The color change of skin graft was observed at different time points. Meanwhile, whether hematocele and fluidify as well as binding to wound appeared under the skin graft were investigated.

所有大鼠背部制作(1.5×1.5)cm2面积的自体全层皮肤移植模型，在不同时间点观察移植皮片的颜色改变，移植皮片下是否有积血、积液，皮片是否与创面粘合。

Thirty adult SD rats were chosen as experimental model and 20 mm length of left sciatic nerve was cut and repaired.

实验分3组：无细胞基膜管移植组、自体神经移植组和异体神经移植组。

We examined 8 cases after costal cartilage autograft in clinical observation through optics microscope, verified that the framework keeps the nature of costal cartilage and pointed out the importance of prevention of infection. Ear reconstruction can be completed in preschool age because we observed that the reconstructed ear in preschool age could grow with the normal ear in pace (7 cases). With perichondrium costal cartilage autograft could live and grow better.

临床观察8例肋软骨移植后的组织学检查，证实了耳支架成活后保持了肋软骨的生物学特性；强调了预防感染对移植耳支架的转归及治疗效果的重要性；观察到7例学龄前儿童自体肋软骨移植后再造耳廓能随年龄同步按比例生长，表明耳廓再造可在学龄前6岁时完成。

On day 7, 15, 30 after injection, the renascent blood vessels were more in number, the KDR area density and the KDR mRNA expression in splenic tissues were higher in VEGF group than the control group, while on day 60 there were no significant differences in the area density of renascent blood vessel, the KDR area density and the KDR mRNA expression between the two groups.

外源性VEGF能够促进移植脾组织内血管生长，自体脾组织移植术后7~15 d开始静脉应用VEGF是促进血管再生的较理想时间；外源性VEGF可能是通过与移植脾组织内所表达的KDR结合发挥作用。

Objectives:(1) to introduce immunetolerance in mice sciatic nerve allograft by intrathymic injection of allogene.(2) to compare the effect of fresh sciatic nerve allotransplantion and cold preserved sciatic nerve allotransplantation with different diameter.

研究目的：(1)探讨小鼠胸腺内注射异基因抗原在同种异体异基因坐骨神经移植免疫耐受中的作用；(2)比较不同直径的新鲜自体神经和冷冻异体神经移植的效果，探讨神经直径对神经移植效果的影响。

Egr-1 mRNA and Egr-1 protein hadn't been found in the normal vein. The expressions of Egr-1 mRNA and Egr-1 protein had biphasic changes. By reverse transcription-PCR and in situ hybridization, we found that the level of Egr-1 mRNA rose at 1 hour after graft, the expression of Egr-1 mRNA was (35±7)%. Decline at hour 6, 24 and day 3, the positive rates of Egr-1 mRNA were (8±2)%,(8±6)% and (8±4)% respectively. Reincrease at day 7, a peak at day 28, the positive rate of Egr-1 mRNA was (45±6)%(compared with other phase, P＜0.01). At day 42, the expression of Egr-1 mRNA declined again. Immunohistochemical staining and Western blot revealed Egr-1 protein had expressed at hour 2 early phase, the expression of Egr-1 protein was (30±5)%, and until to hour 6. The level of Egr-1 protein was decrease at hour 24 and day 3, the positive rates were (7±3)% and (7±8)% respectively.

结果 自体静脉移植后，Egr-1 mRNA和Egr-1蛋白的表达呈双相变化，即移植后1 h， Egr-1 mRNA表达迅速升高，阳性率为（35±7）%，6 h、24 h及3 d时下降到较低水平，阳性率分别为（8±2）%、（8±6）%和（8±4）％，7 d时又再升高，28 d时达高峰，阳性率为（45±6）%，此与其余各时点比较差异均有统计学意义（P＜0.01），42 d时，Egr-1 mRNA的表达再次下降；移植早期（2 h）即有Egr-1蛋白的表达，阳性率为（30±5）%，并持续至6 h，24 h～3 d表达下降到较低水平，阳性率分别为（7±3）%和（7±8）%，7 d时又再升高，至移植后28 d，Egr-1蛋白的表达阳性率达到高峰，为（40±9）%，此与其余各时点比较差异有统计学意义（P＜0.01）。

Methods A retrospective analysis was performed in 14 cases of urinary obstruction among 1090 cases of transplanted kidney in the year 2000-2006, including 9 cases of vesicoureteral anastomotic stricture, 6 of whom received a second vesicoureteral anastomosis, one had infection surrounding the renal graft and ureter end necrosis, two ureteral anastomosis with bladder muscle flap, and one stenostomia aerocyst distention under ureteroscope.

回顾总结2000-2006年我院1090例肾移植患者中的14例移植后上尿路梗阻患者，其中输尿管膀胱吻合口狭窄9例，6例行膀胱输尿管二次吻合手术，1例移植肾周感染输尿管末段坏死采用移植肾近端新鲜存活输尿管与自体输尿管吻合，1例采用膀胱肌瓣代移植输尿管，1例采用输尿管镜下气囊扩张后放置双J管。

RESULTS: Transplanted AS had a favorable fusion between tissue-engineered skin and self-skin on the 3rd day after grafting with a few of ingrowths of capillaries.

结果：AS组在移植第3天，移植的组织工程皮肤与自体皮肤能够很好地融合，有少量毛细血管长入移植物，皮肤移植物颜色与自体皮肤颜色接近；随着时间的延长，移植物中毛细血管的数量逐渐增多，表皮层清晰可见基底层、棘细胞层、颗粒层和角质层，角化现象加强，有角化物脱落；真皮层细胞数量增多，网架逐渐降解，分泌的细胞外基质成份增多；第14天，创面基本愈合；修复区皮肤颜色与正常皮肤颜色非常接近，瘢痕很小，无需进行二次植皮。

The quantity of CD3+ cells in the EdNSC, AdNSC of cloning goattransplantation group and control group had no statistics difference(P＞0.05) at acute and chronic period.Overall, in our study, We got the conclusions as follow: 1,The EdNSC, AdNSCof cloning goat and the AdNSC of the non-cloning goat were successful obtained;EdNSC growed faster than AdNSC in the same cell culture method. 2,The braincontusion and laceration model of cloning goats were successful established by freefalling of 50 gram of steel ball accelerate strike on the goat brain from 100cm height.3, EdNSC and AdNSC transplantation both had obvious therapeutic effect on braincontusion and laceration of cloning goats; The therapeutic effect of EdNSCtransplantation was better. 4, Allograft of stem cells has acute and chronicimmunological rejection and autografting of stem cells has no acute and chronicimmunological rejection.

在相同的条件下，EdNSC体外培养速度比成AdNSC快。2对羊脑上薛氏沟周围运动皮层区使用50g×100 cm力度作自由落体加速撞击，可产生观察指标明确且存活率高的羊脑挫裂伤模型，有望广泛用于神经干细胞移植及药物等治疗的实验研究。3胚胎神经干细胞及成体神经干细胞移植治疗脑挫裂伤后均有较明显的治疗效果；二者相比较而言胚胎神经干细胞稍好于成体神经干细胞。4中枢神经系统异体神经干细胞移植存在着明显的免疫排斥反应，而自体神经干细胞移植基本无免疫排斥反应

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。