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OBJECTIVE To evaluate the myocardial protective effect of self-blood cardioplegia in infants with congenital heart disease in cardiac operation.

目的 探讨自体冷血停搏液对婴幼儿先天性心脏病体外循环中心肌保护作用。

OBJECTIVE To evaluate the myocardial protective effect of self-blood cardioplegia in infants with congenital heart disease in cardiac operation.

目的探讨自体冷血停搏液对婴幼儿先天性心脏病体外循环中心肌保护作用。

Platelet-rich plasmais obtained from autologous blood after centrifugalization in a cell separator.Originally,PRP was used in clinic as a biologic plasma and barrier velum,in order to enhance the effect of hemostasia.

中文摘要:富血小板血浆是自体血离心后的产物,其最初被作为一种生物凝胶和屏障膜应用于临床,主要作用为提高止血效果。

Graves' ophthalmopathy is a common autoimmune disorder that display with Graves' disease showing characteristic clinical feature such as proptosis,eyelid retraction and chemosis.

葛瑞夫兹氏眼病变为伴随葛瑞夫兹氏症常见的自体免疫疾病,其主要临床症状包括眼突、眼睑外翻及结膜水肿。

In order to repair chondral defects, autogenous chondrocyte transplantation is becoming encouraging treatment concerned by most investigators.

在软骨组织修复的治疗方法中,自体软骨细胞移植正成为目前研究和应用的重要方法。

In summary, hyaluronic acid and autologous synovial fluid induces chondrogenesis of equine mesenchymal stem cells, which encourage tissue engineering applications of MSC in chondral defects, as the natural environment in the joint is favorable for chondrogenic differentiation.

总之,透明质酸和自体关节液诱导马的间充质干细胞的软骨形成,这鼓励组织工程在软骨缺损修复中应用间充质干细胞,因为关节自然环境是有利于软骨形成分化。

Autologous MSC can be obtained easily,their source in human are plenty , own active proliferationand potential to difference into chondroblast.

自体骨髓基质干细胞来源丰富,取材容易,具有旺盛的增殖能力及向成软骨细胞分化的潜能,已成为软骨组织工程的首选种子细胞,但MSC诱导分化为成软骨细胞且应用于软骨组织工程的技术尚不十分完善,目前主要采用的技术为细胞团块离心管培养,其构建的软骨组织在力学性能上很难达到关节软骨的要求。

Autologous MSC can be obtained easily, their source in human are plenty, own active proliferation and potential to difference into chondroblast. Now MSC become the chief seed-cell in cartilage tissue engineer, but the technology to stimulate MSC differentiate into chondroblast is not very successful, the standard technology is culture the cell-ball in centrifuge tube, the reconstructured cartilage-like tissue is not achieve the mechanics of hyaline cartilage.

自体骨髓基质干细胞来源丰富,取材容易,具有旺盛的增殖能力及向成软骨细胞分化的潜能,已成为软骨组织工程的首选种子细胞,但MSC诱导分化为成软骨细胞且应用于软骨组织工程的技术尚不十分完善,目前主要采用的技术为细胞团块离心管培养,其构建的软骨组织在力学性能上很难达到关节软骨的要求。

Autologous MSC can be obtained easily,their source in human are plenty , own active proliferationand potential to difference into chondroblast. Now MSC become the chief seed-cell in cartilage tissue engineer , but the technology to stimulate MSC differentiate into chondroblast is not very successful,,the standard technology is culture the cell-ball in centrifuge tube,the reconstructured cartilage-like tissue is not achieve the mechanics of hyaline cartilage.

自体骨髓基质干细胞来源丰富,取材容易,具有旺盛的增殖能力及向成软骨细胞分化的潜能,已成为软骨组织工程的首选种子细胞,但MSC诱导分化为成软骨细胞且应用于软骨组织工程的技术尚不十分完善,目前主要采用的技术为细胞团块离心管培养,其构建的软骨组织在力学性能上很难达到关节软骨的要求。

Methods Autologous BMMSC of New Zealand rabbits were acquired and induced into chondrogenic differentiation by adding transforming growth factor-β1 (TGF-β1), dexamethasane and ascorbic acid in vitro. Then the chondrocytes were implanted onto novel polymeric scaffolds made by Poly (dl-lactide-co-glycolide) embedding with chitosan nonwoven cloth. The tissue engineering cartilaginous grafts composed of chondrogenetic BMMSC/scaffolds were finally transplanted into the defects of ear cartilage in rabbits of the experimental group.

取新西兰大白兔BMMSC体外培养扩增,以含转化生长因子(TGF-β1)、地塞米松和维生素C的培养液做诱导培养,实验组以诱导后的软骨细胞与聚丙交酯-乙交酯共聚物Poly(dl-lactide-co-glycolide)包埋甲壳胺无纺布的新型支架形成复合物修复兔自体耳廓软骨缺损;对照组以PLGA/甲壳胺无纺布支架修复兔耳廓软骨缺损。

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