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Between P14 and P21,CIAPIN1 immunoreaction in the brain,heart and liverbecame much lower. However,between P21 and P28,CIAPIN1 immunoreactionin the heart,brain,liver and skeletal muscle became much lower,while with thekidney development,CIAPIN1 immunoreaction in the kidney became higher. Invarious tissues from adult mouse,CIAPIN1 immunoreaction could be seen incardiac muscle cell,brain,hepatocyte,epithelium of renal tubule,skeletal muscle,lung tissue,gastric mucosa and gland,acinus lienalis.2. Distribution of CIAPIN1 in normal fetal and adult human tissuesTo reveal the possible physiological role of CIAPIN1,we examined theexpression and distribution of CIAPIN1 in fetal and adult human tissues usingimmunohistochemistry. We found that CIAPIN1 was ubiquitously distributed infetal and adult tissues,and was localized in both the cytoplasm and the nucleus.

然而,在3个月大的成年鼠中,CIAPIN1阳性反应物在心、脑、肝和肾小管中的表达强度要低于P28小鼠;但CIAPIN1阳性反应物在成年鼠骨骼肌中较P28小鼠高。2、CIAPIN1蛋白在人5个月胚胎及成人多器官组织内的表达在人5月胚胎多器官组织中,CIAPIN1阳性反应物见于心脏、胆囊单层柱状上皮和粘膜、结肠粘膜、小肠粘膜和绒毛、肝脏、直肠腺体、胃粘膜、肾上腺束状带、甲状腺滤泡、脾索、胸腺小叶间隔、皮肤真皮层和汗腺、睾丸白膜和间质、脑组织内神经元和神经胶质、肺小支气管和肺泡、骨骼肌、肾脏皮髓质和肾小管、子宫内膜、胰腺腺泡和胰岛、卵巢、输卵管粘膜等绝大多数组织细胞。

The two membranes with immunoreagents are cut into strips and are contiguously attached in length (Fig. 1A), and a cellulose membrane is located at the top to induce a continuous wicking that enables the immune complex to be pulled to the immobilized antibody.

带有免疫试剂的两个膜被割成条状,在长度方向连续的粘贴上(Fig.1A),位于顶端的纤维素膜用来产生能将免疫复合物拉到固定化抗体的连续毛细作用力。

Martensia was characterized by either single or multiple network-banded blades, procarps consisting of a four-celled carpogonial branch flanked on one side by a single lateral sterile group and on the other side by a group of cover cells, and cystocarp scattered over fertile blades.

此属的特点为藻体膜叶状且膜叶具有单或多层的网状结构,其雌性殖果孢枝特化具有侧边单一的不孕枝而另一边有一群覆盖细胞,果孢子囊散生在藻体各处。

Leaf blades mainly basal, filiform to setaceous, usually involute; ligule a narrow membrane fringed with hairs.

叶片主要基部,对刚毛状,通常内卷丝状;一层狭窄的膜用头发作为边缘的叶舌。

Filaments free, very short; anthers free or mostly connivent into a sheath around ovary, 2 anterior ones with spurlike or wartlike and nectariferous appendages at base, these extending into anterior spur, connectives produced apically into conspicuous, membranous appendages.

花丝离生,非常短;花药离生或多数在子房周围靠合成为鞘,前面2枚基部具距状或瘤状和分泌蜜汁的附属物,这些延伸进入前面的距中,药隔顶部生明显、膜质附属物。

Fruit dehiscent siliques or indehiscent silicles, linear, oblong, or ovoid, strongly latiseptate, terete, or 4-angled, sessile; valves obscurely veined, tomentose, smooth or torulose, subapically 4-horned; replum flattened; septum complete, membranous; style absent, obsolete, or distinct; stigma capitate, entire.

果开裂长角果或不裂短角果,线形,长圆形,或卵球形,强烈具宽隔膜,圆柱状,四棱形的或,无梗;裂爿不明显脉,被绒毛,平滑或,4有角;使变平的假隔膜;隔膜完全,膜;花柱无,废退,或者离生;柱头头状,全缘。

Seed horizontal or vertical, lenticular, reniform, ovoid, or globose; testa thinly leathery or membranous; embryo green or whitish, planospiral, slender; perisperm scant or absent.

种子水平或垂直位置,透镜状,肾形,卵球形,或球状;种皮薄革质或者膜;胚绿色的或带白色,平面螺旋状,纤细;外胚乳不够的或无。

Corona distinctly cupular or cylindric, usually obviously membranous, sometimes plicate, tube as long as or longer than gynostegium and/or much longer than marginal lobes.

清楚的副花冠杯状或圆筒状,通常明显膜质,有时折扇状,筒部等长或长于合蕊冠和远长于边缘的裂片。

METHODS: Fresh human amnions were clipped to pieces which were sutured in a shape like muff by utilizing epidural tubes as a stent. When 4 cm section of ureter in length was excised to replace the defected ureter. 36 rabbit models were divided medially into the 1-month therapy and 3-month therapy groups. At the end of study, all amnion-ureter were sliced and examined with pathologic method or immunohistochemistry to check expressions of α-actin in smooth muscle cells and CKAE1/AE3 in transitional epithelial cells.

新鲜人羊膜裁剪成片状,以硬膜外导管为支架,做成袖套状,替代4 cm的大白兔输尿管缺损。36只成模大白兔均分为1个月治疗组和3个月治疗组,实验结束时,取羊膜输尿管行病理学检查和免疫组织化学检查平滑肌细胞α-Actin和移行上皮细胞CKAE1/AE3的表达情况。

A: normal growth of blood vessels in chick chorioallantoic membrame; B: blood vessel net in a spoked wheel pattern; C: vasoproliferative response comparable to that induced by VEGF Original magnification:×6.1

对照组CAM血管形态呈自然生长状态下的叶脉状(图2A), 40 μg/ml PMP组与对照组无差别,80 μg/ml PMP组及VEGF组在混合纤维素滤膜周围可见到不同程度放射状密集生长的血管网(图2B、2C),而对照组及低

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