膜片

In order to elucidate the effect of membrane stretch on ionic currents, we employed the whole-cell patch-clamp technique to investigate the effects of different kinds of stretch on voltage-dependent calcium currents in antrial circular smooth muscle cells of the guinea-pig.

利用全细胞膜片钳技术，在胃窦环行肌细胞上观察了不同方式的牵张刺激对电压依赖性钙电流的影响，探讨牵张刺激对胃窦平滑肌细胞电压依赖性钙电流的作用。

In order to elucidate the effect of membrane stretch on ionic currents, we employed the whole-cell patch-damp technique to investigate the effects of different kinds of stretch on voltage-dependent calcium currents in antrial circular smooth muscle cells of the guinea-pig.

利用全细胞膜片钳技术，在胃窦环行肌细胞上观察了不同方式的牵张刺激对电压依赖性钙电流的影响，探讨牵张刺激对胃窦平滑肌细胞电压依赖性钙电流的作用。

To verify our speculation, we investigated the functions of the voltage-dependent potassium channel and large conductance calcium-dependent potassium channel of VSMCs isolated from hindquarter arteries and cerebral arteries of rats after short- and mid-term simulated weightlessness and compared with that of control rats, using both pharmacological methods and whole cell patch clamp techniques.

为证明我们的推想，我们采用动脉血管环药理学实验方法及全细胞膜片钳技术，来观察模拟失重大鼠脑部及后身动脉VSMCs的两种钾离子通道电压依赖性钾离于通道LK＊与大电导钙激活钾离于通道［BK＊）功能的改变并注意此变化的时程特征。

Results from whole-cell patch clamp showed: 1 BmK Ⅰ(α-like neurotoxin) was found to prolong the duration of action potential and promote the amplitude of action potential. The effects could be attributed to the selective action of BmK Ⅰ on the TTX-S Na〓 channel: slowing the inactivation process of Na〓 channel, increasing the amplitude of peak Na〓 currents; 2 BmK abT, a new-type neurotoxin with higher sequence homology to β-type scorpion neurotoxin, was found to slow the inactivation process of Na〓 channel and prolong the duration of action potential in DRG neurons.

全细胞膜片钳研究发现：1）类α型哺乳动物毒素BmK Ⅰ可延长部分DRG神经元全细胞动作电位的时程，增加动作电位的幅度，这些效应可归咎于BmK Ⅰ选择性地作用于DRG神经元TTX—S钠通道，延缓TTX—S钠电流的失活化相，峰钠电流幅度增加；2）BmK abT，一个与β型毒素同源性较高的新型蝎毒素可抑制DRG神经元全细胞钠电流失活化过程，使动作电位时程增加。

The whole-cell patch clamp technique was used to explore the effects of leptin on the VGCC in long-term cultured hypothalamic neurons.

2采用全细胞膜片钳技术，研究瘦素对不同培养时间的下丘脑神经元电压依赖性钙通道的作用。

This experiment aimed to investigate the modulation of hypotonicity on ATP-activated currents in primary sensory neurons. Whole cell-patch clamp recording was performed on cultured SD rat trigeminal ganglion neurons.

本实验应用全细胞膜片钳技术，在大鼠三叉神经节神经元上探讨低渗透压对ATP-激活电流的调制作用。

RESULTS摘要: Under isotonic conditions, no single channel activities were observed in most patches. Hypotonic superfusioninduced cell swelling resulted in the appearance of active channel. Under symmetrical condition, the current induced by hypotonicity showed outward rectification and an unitary conductance of （38.1±2.5）pS.

结果摘要：等渗状态下，用细胞贴附式记录，多数心肌细胞膜片无通道活动，用低渗溶液灌流细胞，在细胞容积增大的同时，出现单通道电流的激活。

Hypotonic superfusioninduced cell swelling resulted in the appearance of active channel. Under symmetrical condition, the current induced by hypotonicity showed outward rectification and an unitary conductance of （38.1±2.5）pS.

结果： 等渗状态下，用细胞贴附式记录，多数心肌细胞膜片无通道活动，用低渗溶液灌流细胞，在细胞容积增大的同时，出现单通道电流的激活。

Hypotonicity,TRPV4receptor,ATP-activatedcurrents,trigeminalganglion,patch-clamptechnique

TRPV4受体，低渗透压，ATP-激活电流，三叉神经节神经元，全细胞膜片钳

Do not insert any hard objects into the pressure hole of the transmitter or directly contact the sensor membrane otherwise damaging the sensor.

请勿将坚硬物体插入变送器的压力测量孔内或接触传感器膜片，以免损坏传感器芯片。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。