膜片

The hFCECs were cultured by sticking tissues piece method that the cornea were divided into the limbus and central tissue piece and digestive method,respectively.For digestive method,the digestive juice of 5mg/ml DispaseⅡ+0.25%trypsin was chosed.D/F12 with 10%fetal bovine serum and 100 U/ml penicillin and streptomycin were used in this study,the culture condition was 37℃and 5%CO2,and culture medium changed every three days2、Passage of hFCECsAfter more than 80%confluenced,cells from corneal limbus hFCECs were digested for 5-15min with different concentrations of trypsin/EDTA at 37℃,and then passaged at a ratio of 1:2.The cells were subcultured on empty plates,mouse 3T3 fibroblast feeder layer,fetal corneal stromal cell feeder l ayer or HTK feeder layer respectively.And D/F12, mouse 3T3 fibroblast conditioned medium,fetal corneal stromal cells conditioned medium or HTK conditioned medium with 10%FBS and 100 U/ml penicillin and streptomycin were used respectively as the culture medium.And the culture medium were changed every three days as well.

方法一、人胎儿角膜上皮原代和传代培养1、原代培养严格无菌操作获取人胎儿角膜片，采用组织块贴壁法、酶消化法原代培养人胎儿角膜上皮细胞。2、传代培养角膜缘部的细胞生长融合达80%以上，不同稀释浓度的胰酶/EDTA消化液消化传代分别接种于空板、小鼠3T3成纤维细胞饲养层、胎儿角膜基质细胞饲养层及HTK饲养层，培养液分别采用D/F12、小鼠3T3成纤维细胞条件培养液、胎儿角膜基质细胞条件培养液、HTK条件培养液。

Consulting the relevant information, the engine CZ4102Q made in Changzhou Diesel Engine Factory is chosen according to the full-load speed characteristic. The clutch adopts dry single-plate diaphragm spring one which is operated by hydraulic pressure system. The choices and arrangements of rear axle, steering gear, propeller shaft, differential and brake, etc is referred to the related parts on BJ1041 light truck.

发动机的选择是经查阅资料在其现有的外特性曲线上选择的常州柴油机厂CZ4102Q 发动机；离合器的改装设计选择了膜片弹簧液压式操纵机构单片干式离合器；后桥、转向器、传动轴、差速器和制动器等部件的选择和布置参照了BJ1041 轻型载货汽车的相应部件选择和布置设计。

When the condensate water temperature gradually increased, the membrane began to evaporate liquid-filled box, the membrane box pressure rise, driven diaphragm valve heart Needle Valves to move off in the condensate to saturation temperature before the trap is completely closed, the membrane box with the steam temperature change control valve switch, play a role in blocking the drainage steam.

当冷凝水温度渐渐升高，膜盒内充液开始蒸发，膜盒内压力上升，膜片带动阀心向关闭方向移动，在冷凝水达到饱和温度之前，疏水阀完全关闭，膜盒随蒸汽温度变化控制阀门开关，起到阻汽排水作用。有开阀温度低于饱和温度15℃和30℃两种膜盒供选择。& bellows steam trap &是由中国疏水阀网提供的国际阀门新闻，译文仅供参考。

Pressure valve is of direct action type, which is composed of body, seat, core parts, bonnet, highest quotation, diaphragm, spring, adjusting nut and upper and bottom diaphragm cover etc..

本产品为直接作用型自力式压力调节阀。由阀体、阀座、阀芯部件、阀盖、顶盘、上、下膜盖、膜片、弹簧、调节螺母等零部件所组成。

Reflection of a beam of light on the diaphragm falls on a phototransistor.

经膜片反射的光束照射到光敏管上。

METHODS摘要: The cardiac myocytes were isolated from mouse hearts and put in a hypotonic solution. Cellattached and insideout patch clamp methods were employed to recorll the swellingactivated Cl- channel current.

方法摘要：采用膜片钳单通道（细胞贴附式和内面向外式）方法，记录急性分离的小鼠心肌细胞暴露于低渗溶液时出现的肿胀激活氯通道电流。

METHODS: The cardiac myocytes were isolated from mouse hearts and put in a hypotonic solution. Cellattached and insideout patch clamp methods were employed to recorll the swellingactivated Cl- channel current. RESULTS: Under isotonic conditions, no single channel activities were observed in most patches.

采用膜片钳单通道（细胞贴附式和内面向外式）方法，记录急性分离的小鼠心肌细胞暴露于低渗溶液时出现的肿胀激活氯通道电流。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。