膜片

The expression of human Kv1.3 gene was detected by fluorescence microscope and whole cell patch clamp.

结果：pcDNAHKv1.3真核表达载体转染HEK293细胞2 d后，荧光显微镜可观察到人Kv1.3通道表达；3 d后全细胞膜片钳技术记录到人Kv1.3通道基因编码的通道电流。

Methods: MTT assay was used to determine the inhibition of TAM on cell proliferation. The whole cell patch clamp technique was used to observe the effects of tamoxifen on Icl,vol.

MTT法检测TAM对细胞增殖的作用；流式细胞术检测TAM对细胞周期的影响；全细胞膜片钳技术观察TAM对容积激活性氯电流的抑制作用。

Methods Whole cell patch clamp technique was used to study the changes of voltage dependent calcium current of 22 neurons in caudate nucleus before and after morphine treatment.

采用膜片钳全细胞记录模式观察了 2 2例尾核神经元电压门控性钙通道在吗啡给药前后的电流变化。

Responses of DS cell to moving light bar in different directions were recorded using whole cell patch clamp technique on the rabbit retina.

在视网膜上用膜片钳的技术记录DSGCs 对在不同方向上运动的光条的反应。

The experiment was performed on neurons freshly isolated from rat DRG by using whole cell patch clamp techniques.

应用全细胞膜片钳技术在大鼠新鲜分离DRG神经元上观察多巴胺D1、D2受体选择性激动剂SKF38393和R-NPA对GABAA受体激活电流的影响。

Methods Enzyme digestion was used to isolate single ventricular myocyte and whole cell patch clamp technique was used to record potassium current.

方法酶解法分离豚鼠单个心室肌细胞，应用全细胞膜片钳技术记录延胡索乙素对豚鼠单个心室肌细胞钾电流的影响。

Whole-cell patch clamp and cell volume measurement techniques were used to investigate the ATP-activated chloride current and the ATP effect on cell volume in nasopharyngeal carcinoma cells.

采用全细胞膜片钳技术和细胞容积测量技术，在低分化鼻咽癌细胞株CNE--2Z上观察ATP诱导的Cl~电流的特性及其对细胞容积的影响。

Heart failure ; Pneumonia ; Whole cell clamp ; Calcium ion ; Mechanism

心衰；肺炎；全细胞式膜片钳；钙离子；发病机制

Methods Whole cell patch clamp technique was used to test the effects of Pho and Fen on TTX-S and TTX-R sodium currents in DRG neurons.

应用膜片钳技术研究氰戊菊酯和辛硫磷对DRG河豚毒素敏感和不敏感型钠通道的作用。

Whole cell patch clamp technique was used to investigate the effect of sinomenine on sodium current and L-type callium in isolated guinea pig ventricular myocytes.

应用膜片钳全细胞记录技术研究青藤碱对酶解分离的豚鼠单个心室肌细胞膜钠离子电流、L型钙电流的影响。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。