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Using visual patch-clamp method synaptic transmission can be accurately assessed by measuring postsynaptic currents of the dorsal horn neurons.

用可视膜片钳方法可以准确测量脊髓背角神经元的突触后电流,从而研究初级传入突触的传递过程。

Whole-cell recordings were performed to record miniature excited postsynaptic currents after neurons were cultured for 1 week and 2 weeks.

培养到1周和2周时,采用膜片钳全细胞模式记录神经元突触内自发的微小兴奋性突触后电流。

Methods Rat DRG neurons were isolated mechanically and enzymatically, on which whole-cell patch clamp recording and repatch technique for intracellular dialysis were performed.

方法在新鲜分离的大鼠DRG神经元标本上,以全细胞膜片钳技术记录膜电流,用排管快速换液装置行胞外给药,以胞内透析技术分析信号转导途径。

Their hearts were cannulated via aorta and mounted on a Langendorff apparatus and perfused retrogradely. Single midmyocardial cells were isolated enzymatically from rabbit left free wall, I were recorded by using patch clamp techniques in the whole cell configuration before and after perfusiong of 10^(-6)mol/L and 10^(-5)mol/L ibutilide solution 10^(-6 group and 10^(-5) group.

新西兰纯种大耳白兔60只,取其心脏行Langendorff灌流,以酶解法分离出单个左心室中层心肌细胞,以10^(-6)mol/L(10^(-6)组)及10^(-5)mol/L(10^(-5)组)伊布利特外液灌流正常左室前壁中层细胞,以膜片钳技术记录I的活性,并与正常对照组进行比较。

Alternative embodiments of the insertion tool utilize severable fibers, a transparent split-mylar sheet or diaphragm, a cantilever fork, a collet assembly or an elastomeric grip collet to gently hold and release the shunt during insertion.

插入工具优选的实施例利用可分离纤维束,透明的分离聚脂薄膜片或隔膜片,悬臂叉,夹头组件或弹性爪夹头,在插入中轻轻固定和释放分流管。

The subendocardial and sub-epicardial tissues were separated from the mid-layer region with opthalmic scissors. Single cells in each region were obtained by gentle shaking and dispersing in a chamber filled with normal Tyrode's solution. The whole-cell patch clamp technique was used to record the current while change the composition of the perfusate.

采用胶原酶二步消化法分离兔心肌细胞,用锐利眼科剪分离左室游离壁内、中、外三层心肌,改变灌流液的成份,采用全细胞膜片钳记录离子电流。

METHODS: In rat hippocampal slices, whole cell patch clamprecordings were used to study anesthetic effects on CA1 neuronintrinsic excitability, and population spike recordings wereused to investigate effects on synaptically evoked discharge.

在大鼠海马脑片上采用全细胞膜片钳记录来研究麻醉药对CA1神经元自发兴奋性的作用;采用群峰电位记录来研究对突触诱发放电的作用。

Experiments were performed to study the voltage-dependence of mIPSC frequency and amplitude using patch-clamp technique with whole cell recording in optic tectal slices of Xenopus.

采用全细胞记录膜片钳技术,研究非洲爪蟾脑片视顶盖神经元mIPSC频率和振幅对电压依赖关系。

The isotropy or anisotropy in some physical properties of the amorphous poly films uniaxially drawn at temperatures above its T g and then quenched to room temperature have been studied.

对非晶态聚对苯二甲酸乙二酯膜片经在Tg以上单轴拉伸然后淬冷至室温的试样,其物理性质的各向同性或各向异性进行了研究。

METHODS: Differences of the vasodilating action of Ipt in big artery and small artery were studied using isolated rat tai l artery helical strips and aortic rings, and the effects of Ipt on potassium cu rrents in smooth muscle cells derived from rat tail artery were observed by usin g patch clamp technique.

采用大鼠尾动脉螺旋状血管条和主动脉离体血管环两种组织,对比观察盐酸埃他卡林对大、小动脉扩张作用的药理学特性,并且利用膜片钳技术观察盐酸埃他卡林对大鼠尾动脉平滑肌细胞钾电流的影响。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

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双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。