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In conclusion, we detailedly investigated the variation of membrane system (mainly plasmalemma, amyloplast envelope, ER) with the potassium permanganate fixative method, nucleus and organelles with routinely fixative method, and their functions with cytochemical method (localization of phosphatases and Ca〓).

综上所述,本文运用高锰酸钾固定法研究了膜系统(主要指质膜、淀粉质体被膜、内质网)的变化,运用常规固定制样研究了细胞核和细胞器的变化,采用细胞化学方法(Ca〓和磷酸水解酶的定位)探索了细胞器和细胞核的功能。

Background Dialysis-related amyloidosis is a serious complication for patients undergoing long-term dialysis. DRA is characterized by amyloid deposition mainly in bone and joint structures, presenting as carpal tunnel syndrome, destructive arthropathy, and subchondral bone erosions and cysts. The pathogenesis of this complication remains unknown.

目的 透析相关性淀粉样变是长期透析病人常见而严重的并发症,淀粉样沉积主要位于滑膜和韧带等骨关节组织,导致侵蚀性关节病变和多发性囊性骨损害等致残性疾病,但其病理机制仍未阐明。

We optimized reaction system for labelling-antibody in which the optimal amount of the purified anti-Stx2B IgG conjugated with colloidal gold beads was 60μg/mL, the purified antibody for E.coli O157 was 57μg/mL, the optimal pH was 8.2, the size of colloidal gold partical was 20nm, the optimization of stabilizing agent was BSA, the optimization buffer was boracic acid buffer with pH 8.2, the optimization of preserving fluid and eluant was boracic acid buffer with pH 8.2 including 5mM NaCl-1%BSA, confining liquid for NC membrane was 0.01% PBST with 3%BSA, the amount of polyclonal antibody against E.coli O157 and Stx2 conjugated with colloidal gold beads for conjugate pad was 3μg respectively, the amount of anti-Stx monoclonal antibody for test line was 0.1μg and 1μg for E.coli O157, the amount of goat anti rabbit IgG for control line of both GICA were 1μg.

测定了胶体金颗粒的最优化反应体系:胶体金颗粒的大小为20nm;抗体与胶体金溶液结合的最佳pH约为8.2;最佳蛋白结合量分别为抗大肠杆菌O157 IgG为57μg/mL,抗重组Stx2B IgG为60μg/mL;最佳稳定剂为BSA;最佳缓冲液为pH8.2硼酸溶液;最佳金标保存液和洗涤液为5mM NaCl-1%BSA的pH 8.2的硼酸缓冲液;NC膜的封闭液为3%BSA的0.01mol/L PBST;Stx2试纸条和大肠杆菌O157试纸条的质控线上的羊抗兔IgG的多克隆抗体最佳点样量均为1μg,其检测线的抗Stx2的单克隆抗体的点样量为0.1μg、抗大肠杆菌O157的单克隆抗体的点样量为1μg,结合垫上的金标抗大肠杆菌O157和重组Stx2B多克隆抗体点样量均为3μg。

To this end, the ultrasound cardiograph is filtered and fuzzily boundary enhanced, a threshold is set to obtain a binary image, then the heart's boundary is got through ellipse curve fitting, cubic spline interpretation and smoothing.

首先对超声心动图像进行滤波以消除图像中的噪声,并运用模糊数学理论进行模糊边缘增强,提取超声心动图像的内外膜边缘信息;接著对超声心动图像作二值化处理和椭圆曲线拟合,初步得到心脏的内外膜边缘;再采用相应的三次样条插值和平滑技术,得到光滑、连续的心脏内外膜边缘。

Results The pathological changes in the nuclei mentioned above included the crimple and ruture of nucleus membrane,mitochondria degeneration and other degenerative structure such as plenty of primary lysosomes,agglomeration of lipochrome,Golgi complex degeneration and so on.

结果上述核团神经元的超微结构均发生病理性改变,神经细胞核膜皱缩,核膜凹凸不整,并有局部断裂;线粒体变性,基质浓度降低及空泡化;粗面内质网和高尔基复合体囊腔扩张变性;大量初级溶酶体及脂褐素集聚;出现了髓样体和多泡体等变性结构。

But the macro fauna in orchard was different from others which including Acarina, Collembola, Nematodes and Hymenoptera. The common fauna was also different in different types of soil.

但果园样地优势类群有蜱螨目、弹尾目、线虫类和膜翅目,各试验样地的常见种类也有所不同。

objective to investigate the expression of tlr9 and tlr7 mrna of peripheral blood mononuclear cells in patients with uveitis.

目的 观察葡萄膜炎患者外周血单个核细胞toll样受体9(tlr9)、toll样受体7(tlr7)mrna的表达。

However, there is disagreement about whether membranes should be treated with a wetting agent before they are delivered to the customer or later in the manufacturing process, after the capture line has been applied to the membrane.

在大规模的工业生产中,为保持 NC 膜的显润特性,膜的后处理通常来用一些实践的经验,但是,对于膜在到达客户手里之前及在试剂盒生产中点样T线后,是否进行后处理,仍然存在争论。

When large quantities of viruses are required for vaccine production or chemical studies, the allantoic sac is utilized.

如绒毛尿囊膜是常用的接种部位,用于1、病毒滴定2、病毒形态学研究3、病毒鉴定,几种病毒能在绒毛尿囊膜上产生水泡样损伤,称为痘痕。

ANP and CX43 began to express at 2nd week after induction and increased gradually,about 60% of the resulting myogenic cells were positive at 4th week after induction ,they were negative for uninduced cells.hMSCs'surface antigen profiles obtained by Flow Cytometry were negative for CD31\CD34\CD45 before and after induction,but CD90 expressed higher after induction while was weak positive before induction(P.05). Apotosis index was correlated with the cultural time after induction,The apoptosis rate of induced hMSCs was remarkably higher than control group(P.01),and the variation between groups was notable(P.05),the cell cycle analysis showed that the percentages of G_0/G_1phases were reduced significantly after induction. The expresstion levels ofβ-MHC and CTNT mRNA were undetectable before induction,began to increase at 1st、4th week after induction,reached the peak at 6th week and decreased after that,the expression of Bcl-2 and Bax mRNA varied regularly after treated with 5-azacytidine. hMSCs'resting membrane potential、range and rate of depolarization were heightened gradually after being induced.

结果:hMSCs诱导前为纺锤形,诱导后第2天部分细胞即开始发生形变,呈球形或短棒状,1周后胞浆中颗粒增多,约20~30%细胞边缘呈毛刷样变化;hMSCs表面抗原CD31、CD34、CD45在诱导前后差异无统计学意义,CD90未诱导时表达呈弱阳性,诱导后明显增高(P.05);ANP和CX43在诱导前无表达,诱导后第2周开始表达且表达随时间逐渐增强,但CX43在诱导后第5周表达量开始降低。hMSCs诱导后凋亡指数随诱导后培养时间增加,低浓度诱导组低于标准浓度诱导组,组间差异有统计学意义(P.05),G_0/G_1期细胞比例诱导后较对照组显著减少(P.05);β-MHC和CTNT基因分别在诱导后第1周和第4周时表达开始增强,在第6周时均达到高峰,第8周时表达开始衰减,Bcl-2、Bax基因表达呈时间依赖性变化,hMSCs经诱导后随心肌样细胞特征的表达膜静息电位、去极化幅值和去极化速率逐渐增高。

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It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发,分析对称连杆曲线上鲍尔点的产生条件,提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品,年生产总值3000万美元,产品价廉物美、选料上乘、质量保证,深受国内外客户的青睐

The eˉtiology of hemospermia is complicate,but almost of hemospermia are benign.

血精的原因很,以良性病变为主。