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The results of physiological indexes analysis under different salt stress condition showed that cell membrane permeability of all species increased along with the increasing of NaCl concentration, and concentrations of Na+, Cl-, proline, soluble sugar, saccharose and malondialdehyde showed the same tendency.

不同盐浓度胁迫下各项生理生化指标的测定结果表明:随着盐胁迫强度的增加,四种植物的膜透性增大,Na+、Cl-、脯氨酸、可溶性总糖、蔗糖和丙二醛含量都呈增大的趋势,高碱蓬和镰叶碱蓬的Pro含量增加迅速,明显比盐角草和盐爪爪高,盐角草Na+、Cl-含量明显高于其它三种植物,K+、Ca2+、Mg2+、叶绿素含量,过氧化物酶、超氧化物岐化酶和过氧化氢酶活性和K+/ Na+都呈下降的趋势。

The results suggest that drug synovectomy may be one mechanism about intra-articular corticosteroid therapy in TMD, and this therapy could lead to progressive degradation and destruction of the articular cartilage.

糖皮质激素通过药物性的滑膜切除是治疗TMD关节源性疼痛的一个可能的作用机制;②一次性的关节腔内注射治疗剂量的糖皮质激素可以导致软骨基质的丢失和软骨的破坏。

Pleomorphic adenoma is the most common gland tumor of parotid origin, accounting for 60 to 70%. This kind of tumor usually presents as a slow-growing painless mass anteroinferior to the ear. The diagnosis is based on physical examination, image studying and even fine needle aspiration biopsy. The optimal method of treatment of such tumors is local excision or paroidectomy in which the entire capsule is totally dissected off but the facial nerve is spared. Diagnosis and treatment planning are focused on preventing tumor recurrence and consequent malignant transformation.

腮腺之多形性腺瘤乃是唾液腺最常见的肿瘤,约占所有唾液腺良性肿瘤的百分之六十至七十,这类的肿瘤在临床上的表现常是耳下缓慢增生的无痛性肿块,临床上诊断的依据必须配合临床检查、电脑断层扫描、磁振造影及细针抽取切片的病理检查方可得知,其治疗方式主要是对肿瘤进行局部切除手术或腮腺的切除手术,手术中颜面神经需小心保留,但须将肿瘤的外膜部分切除乾净,诊断及治疗计画著重在预防肿瘤复发及肿瘤产生恶性转变。

The relative cell groth rate of L929 cell represented the proliferation of cells on materials with respect to control over time. The result showed that cytotoxicity of thermo-sensitive cell culcure dish was none or little,which can be used for lab use.Additional cell culture was performed with two types of celland cell sheets detachment were observed by reducing the culture tempareture after the confluently growth of the two types of cells.

进一步利用两种不同的细胞永生化的成牙本质细胞系和人牙周膜细胞在接枝有PNIAPm的培养皿上进行培养,观察到细胞可以正常贴壁,生长并增殖,并且在形成连续的细胞层之后,通过降低温度,获得了完整的细胞层结构,结合前两部分实验结果,证实了利用等离子体预处理和紫外线照射接枝制备温敏性培养皿的可行性,为后续研究提供了实验基础。

With aggravation of drought stress, ZXY04P-75 and ZXY04P-201 increased in proline content while that of ZXY03P-173 and ZXY04P-239 increased initially, reaching the peak at the 8th day, then declined. And the rate of net photosynthetic declined notably with the intensity of drought stress, The minimum of decline rate of net photosynthetic is ZXY04P-201. 4. The results of photosynthetic mechanism for 4 Dactylis glomerata L. show: the net photosynthetic rate, transpiration rate,stomatal conductance and chlorophyll decreased dramatically, Compared with weak drought resistance ZXY04P-239,the net photosynthetic rate, transpiration rate, stomatal conductance and chlorophyll in the leaves strong drought-resistance ZXY04P-201 decreased slowly.

连续水分胁迫及复水条件下4份鸭茅抗旱机理的研究表明,叶片水分饱和亏缺随着干旱胁迫强度的增加而增加,其中变化幅度最大的是ZXY04P-239,最小的是ZXY04P-201;ZXY03P-173和ZXY04-201质膜相对透性和可溶性糖含量随着干旱胁迫强度的增加而增加,而ZXY04P-75和ZXY04P-239出现单峰变化,在胁迫第8d后达到最大,然后下降;ZXY03P-173和ZXY04P-239脯氨酸含量在干旱胁迫第8d达到最大,而ZXY04P-75和ZXY04P-201的脯氨酸含量随着干旱胁迫程度的加深而逐渐达到最大;4份材料叶片的净光合速率随着干旱胁迫强度的增加而减小,其中ZXY04P-201的净光合速率下降率胁迫至12d时为最小。

CaN Aβ gene silencing can reduce myocardial hypertrophy in cultured cells, si1280 (21bp) of CaN Aβ gene is the most effective target site for siRNA. The method of intrapericardial injection of plasmid, microbubbles and erzymes can improve transfection efficiency of non-viral plasmid with satisfying targeted transfection. But the scope of transfected myocytes is still limited. CaN Aβ shRNA expressing plasmid transfection in vivo by pericardial injection results in decreased CaN Aβ protein expression of small part of myocytes, and CaN Aβ mRNA only shows decreased trend. The dosage of non-viral vector and the parameters of ultrasound energy should be optimized in further study.

结论RNAi技术抑制CaN Aβ基因表达可有效减轻Ald诱导的心肌细胞肥大程度;CaN Aβ基因中si1280(21bp)片段为实现RNAi的更有效片段;微泡+酶类心包腔内注射超声导入的方法可有效改善非病毒载体在体心肌的转染效率,同时具有一定的靶向性,但总的转染范围仍然有限;采用这一方法进一步进行&一肾一夹&心肌肥大模型大鼠在体心肌细胞的CaNAβ的RNAi干预,发现心肌肥大大鼠心外膜下局部心肌细胞CaN Aβ蛋白水平降低,CaN AβmRNA水平虽有下降趋势,但无统计学差异,提示质粒的用量及超声导入的参数有待进一步研究使其优化。

A new picorna-like virus was isolated from dead Evtropis oblique larvae deceased from NPV infection. Electron microscopic observations of purified virions were non-enveloped isometric particles with diameter of 26nm. The virions contain two capsid proteins: 31.5kDa and 28.8kDa, the amount of later is 2.5 times more than that of the former. Analysis of 3′terminal sequence of EoPV clone identified that it can encode RdRp and has eight conserved motifs. Homology analysis shows that it is closely related to Perina nuda picorna-like virus.

从核型多角体病毒感染致死的茶尺蠖幼虫尸体中分离到一株微小RNA病毒透射电镜观察纯化的病毒粒子为无囊膜、无表面特征、直径约26nm的球状颗粒。16%的SDS-PAGE显示它有两个分子量为31.5 kDa和28.8 kDa的衣壳蛋白,后者的含量是前者的2.5倍。3′端克隆序列分析表明EoPV基因组3′有poly尾,编码RNA聚合酶,含有微小RNA病毒RNA聚合酶的八个保守基序,同源性分析表明它与榕透翅毒蛾微小RNA病毒亲缘关系最近。

The results show that with Cd2 + Stress Pistia stratiote large increase of the concentration of leaf tissue of the chlorophyll content decreased, while the MDA, soluble protein content, proline content, lipid membrane permeability, SOD, POD, CAT, nitrate reduction The first enzyme content was increased at different times and reached a peak after the downward trend.

结果显示,随着镉胁迫浓度的增加大薸叶组织内的叶绿素含量呈下降的趋势,而丙二醛、可溶性蛋白含量、脯氨酸含量、脂膜相对透性、超氧化物歧化酶、过氧化物酶、过氧化氢酶、硝酸还原酶的含量呈先升高并在不同的浓度达到高峰后再下降的趋势。

The results showed that there was a more better effect of filmformation and absorbency to use poly 1788 as an adhesive and polyvinyl pyrrolidone(K:90) as a hydrophilic polymer. The silicon dioxide with particle size 0.3-1 μm as a inorganic pigment had a better absorbency. The polyethylene glycol modified with ethylenediamine had a more better characteristic of dye immobilization, absorbency and definition than the polyethylene glycol modified with diethylenetriamine, of which the polyethylene glycol of molecular weight of 400 modifiedwith ethylenediamine had more best performance.

研究结果表明:采用聚乙烯醇1788作黏合剂、聚乙烯吡咯烷酮(K值为90)作亲水性高分子可得到比较理想的成膜效果和吸墨效果;粒径分布范围在0.3-1 μm的二氧化硅作为无机颜料具有较好的吸墨性能;用乙二胺改性的聚乙二醇比用二乙三胺改性的聚乙二醇具有更好的固墨性、吸墨效果以及清晰度,其中乙二胺改性的分子量为400的聚乙二醇所得到的烷基多胺的效果为最好。

In Wistar rats, latent period of a-wave in scotopic rod response after 1-week transplantation was shorten (P.05); and latent period of a-wave in scotopic rod response after 2-week transplantation was prolonged (P.05); while latent period of b-wave in scotopic maximum response after 1-week transplantation was shorten (P.05). In RCS rats, amplitude of a-wave in scotopic rod response and photopic response after 4-week transplantation was increased (PO.05); while latent period of a-wave in scotopic rod response after 4-week transplantation was shorten (PO.05).Conclusion 1. At the age of 3 months, photoreceptors of the RCS rat degenerated completely, which leaded to the reduction of amplitudes of a-wave and b-wave.

Wstar大鼠术后1周的暗适应视杆反应,a波潜伏期缩短o叼。05);术后2周的暗适应视杆反应,a波潜伏期延长o功刀5):术后1周的暗适应最大反应,b波潜伏期缩短o功刀5卜*CS大鼠术后4周的暗适应视杆反应和明适应反应,a波振幅增大o功刀匀;术后4周的暗适应视杆反应,a波潜伏期缩短o<0卜结论1、RCS大鼠在3月龄时感光细胞变性死亡,导致FERG的a、b波振幅明显降低;视网膜下腔仍存在感光细胞外节盘膜碎屑,外丛状层缺乏感光细胞的突触末端,同时Muler细胞出现反应性胶质纤维增生。

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