膜性的

In this thesis the planar bilayer lipid membranes inlaid with oxide cholesterol , phosphatidylcholine or cephalin have been prepared .The effects of membranes formulas on membranes stability and zinc ions transfer ability have also been studied.

中文摘要采用氧化胆固醇与磷脂混合作为成膜液制备双分子层脂质膜的方法，研究了制备平板双分子层脂质膜的配方和成膜条件，并对制得的双分子层脂质膜的稳定性、离子通透性、离子选择通过性能、双层膜中氧化胆固醇对通透性的影响等进行了研究。

The evaluation was made on the basis of the characteristics of conglutination and dissolution, then the best formula was determined finally.

根据正交实验的结果制备的药膜，在粘附性、溶解性方面均符合要求，且在成膜性、膜外观方面均较理想

On the base of theoretical design and analysis, the working state of Water-soluble Filming machine and its laminating system are tested through the equipment applied to do serial experiment. The results of theoretical design and analysis are proved correct and practical, some new problems appear. Meanwhile, the crushing effect and saving energy of the equipment working in process of grinding ironstone are researched, all these lay a foundation for the further study of the technology.

基于机电一体化技术的压合机构控制系统的应用有效地解决了水溶性复膜机研制过程中的关键技术问题—复膜压力精确调整，实现以先进的科技手段解决关键性的技术问题，为水溶性复膜机的广泛推广奠定了基础，增强了水溶性复膜机的市场竞争能力，能在产业化、系列化生产中收到很好的社会效益和经济效益。

Membrane affinity quantified by IAM and liposome/buffer systems was a more efficient predicator of α values than hydrophobicity from n-octanol/buffer system. Consequently, not only hydrophobic force but also attractive polar extra-interactions involved in the binding to IAM and liposomal membranes but not in an n-octanol phase, ascribed to the binding process within AM. The binding to ordered lipid membrane in the larger part determined drugs'binding to AM intracellular compositions, and the ordered phospholipid plasma membrane was a potential site for drugs'binding in AM.

脂质体／水系统和磷脂膜色谱所测定的膜亲和性比正辛醇／水系统的疏水性更好的预测巨噬细胞内药物的α值，因此包含在磷脂膜色谱中但并不体现在正辛醇／水系统中药物与磷脂膜之间的额外吸引性极性作用力对药物在细胞内结合有着重要贡献；药物与有序磷脂膜的结合在很大程度上决定着药物与巨噬细胞内成分的结合，有序磷脂分子膜可能是巨噬细胞内部潜在的结合部位。

The UV and IR spectroscopic studies of the monomer films showed that the photoreaction ratio of monomer (about 50%) was higher than the corresponding photosensitive polymer (about 10%, PVCi). The polarized IR spectra proved that obvious anisotropic absorption was displayed in the irradiated photosensitive film. With polarizing microscopy method, we found that these kinds of photoalignment films exhibited good alignment ability, and the stability of the photoalignment film could reach about 100℃. 2 A photosensitive self-assembled monolayer was fabricated that contained photo-cycloadditable groups, such as, coumarin and cinnamate. The reaction process were detected by contact angle method because the IR absorption of monolayer was very weak. The photosensitive self-assembled monolayer promote uniform alignment of nematic liquid crystals by LPUV, and it thermal stability could reach about 130℃. 3 A cationic bolaform amphiphiles was synthesized that had two photosensitive double bonds, and can be polymerized by ultraviolet. A layer-by-layer self-assembled deposition film was prepared in aqueous solution by using this material and poly (sodium 4-styrenesulfonate). The UV-Vis. spectra proved that the multilayers film was uniform and layer-by-layer. When LPUV irradiated the film, the photopolymerizable double bond underwent [2+2] cycloaddition, and the production of the reaction distributed along the polarized direction of incident LPUV. Polarized UV-Vis. spectra indicated that the irradiated self-assembled multiplayer film was anisotropic, and the dichromic ration is about 0.09, it is higher than the spin-coating method of photosensitive polymer or monomer. We used this anisotropic film as alignment layer of nematic liquid crystals, and obtained a uniform and stability alignment result, the static contrast ratio is about 100, which is very close to application of liquid crystal device. This thesis is an instance that the photochemical material was used in the physics region of liquid crystals, the route that was from photosensitive monomer, self-assembled monolayer film to self-assembled multiplayer film was undergone, by this logical research method photoailgnment of liquid crystal was extended and consummated.

围绕上述思路开展了如下工作：1合成了三种可进行光二聚反应的光敏小分子单体，LPUV 辐照制作了光控取向膜；用紫外-可见光谱法和红外光谱法分析了薄膜中的光化学反应，发现小分子的光反应程度可达到50%以上，至少比光敏高分子高出5倍；薄膜的偏振红外光谱证实，经过LPUV 辐照后薄膜出现了明显的各向异性；将小分子光控取向膜制成液晶器件，在偏光显微镜下观察看到均匀一致的液晶取向；并且热稳定性达到100℃左右，接近实用化要求。2制备了含肉桂酸、香豆素基团的自组装单层膜；采用接触角的方法监测自组装反应的动力学过程；紫外-可见光谱证实了自组装单层膜在石英基板表面形成；自组装单层膜经LPUV 辐照后能诱导液晶均匀取向，而且热稳定性可达130℃，热稳定性进一步提高。3合成了含有光敏双键的双季铵盐，在水溶液状态下将它和聚乙烯基苯磺酸钠沉积生成layer-by-layer 自组装多层膜，紫外-可见光谱证实这是一个逐层均匀的沉积；在LPUV 照射下，自组装膜中的光敏双键按照偏振方向发生[2+2]环加成反应，形成取向膜，用二向色性值评价的分子取向度高于高分子及小分子光控取向膜的水平；实验证明用自组装多层膜制成的液晶器件亮暗态均匀，静态对比度约为100，达到实用化器件的水平。

The expression changes of relating-apoptosis gene proteins (bcl-2, bax) were detected by immunohistochemistry before and after treating with the Agaricus Blazei Murill extract to explore the possible mechanisms of inducing apoptosis for MGC-803 cells in vitro. Results: The Agaricus Blazei Murill extract significantly inhibited the proliferation of gastric cancer cells in vitro and the inhibitive effects were depended on the medicine concentration and treating times. After treating 24 hours on the gastric cancer cells with the morphologic changes of apoptosis with chromatin margination, karyopyknosis, karyorrhexis were found by the light.

结果：(1)通过细胞培养和MTT法，表明长白山姬松茸在体外对MGC-803细胞株有明显的抑制作用，加药组与对照组相比其生长抑制率有显著性差异(P＜0.01)，而且这种抑制作用呈现浓度和时间的依赖性；(2)通过集落形成率的测定结果表明，加药组与对照组相比其集落形成率和集落形成抑制率有明显差异(P＜0.01)，说明长白山姬松茸对MGC-803胃癌细胞株有明显抗增殖作用；(3)光镜观察结果表明，加药组可见细胞脱水浓缩伊红染色增强，胞体缩小，内含高度浓缩的胞核呈深蓝色等典型细胞凋亡形态；经AO／EB荧光染色观察结果表明，当终浓度为1.0mg／ml的姬松茸提取物作用于MGC-803胃癌细胞24h后，其凋亡率和破膜率与自然凋亡率与破膜率相比，均有显著性差异，其凋亡率86.3％(P＜0.001)，破膜率为41.6％(P＜0.01)，说明姬松茸确实有诱导MGC-803胃癌细胞凋亡的作用，同时也有细胞毒作用，但以诱导细胞凋亡为主；(4)免疫组化结果表明，用药前后凋亡相关基因的BCl-2、Bax蛋白均有显著性的改变(P＜0.001)。

The main target of PDT-CPD4 was probably membrance organelles, such as cytomembrance、lysosome、microsome. It made lipide and protein of these membrance dissociate and degenerate, membrance penetration increased. Typical biochemical changes appeared, 180bp bases DNA fragmentation ladder pattern was observed in agar electrophoresis.

但PDT作用后出现线粒体肿胀，可能是因为PDT的主要靶部位是膜性细胞器如线粒体、胞质膜、内质网和溶酶体等，光化学反应产物使这些膜的脂类和蛋白质发生分解变性，膜通透性增高所致。

In this review data on the use of rituximab is presented from 92 studies involving 1197 patients with the following diseases: systemic lupus erythematosus, idiopathic thrombocytopenic purpura, anti-neutrophil cytoplasmic antibody associated vasculitis, Grave's disease, autoimmune hemolytic anemia, pemphigus vulgaris, hemophilia A, cold agglutinin disease, Sjogren's syndrome, graft vs. host disease, thrombotic thrombocytopenic purpura, cryoglobulinemia, IgM mediated neuropathy, multiple sclerosis, neuromyelitis optica, idiopathic membranous nephropathy, dermatomyositis, and opsoclonus myoclonus.

本综述资料总结了92项共1197例患者应用利妥昔的研究，包括系统性红斑狼疮、特发性血栓性血小板减少性紫癜，抗中性粒细胞抗体相关性血管炎、甲状腺功能亢进、自身免疫性溶血性贫血、寻常型天疱疮、甲型血友病、冷凝集素病、干燥综合征、移植抗宿主疾病、血栓性血小板减少性紫癜、IgM介导性神经病、多发性硬化、视神经脊髓炎、特发性膜性肾病、皮肌炎和眼肌阵挛性肌阵挛。

Experimental results revealed that triethanolamine has remarkable effect on arc restraining,which ...

实验结果表明：三乙醇胺可以有效抑制火花放电，增加膜层的厚度，使表面孔隙变小，提高表面光洁度；当三乙醇胺浓度为30g.dm-3时，膜层耐蚀性能最好；在阳极氧化过程中，三乙醇胺化学吸附于镁合金表面，从而改变微弧氧化过程中氧气气泡在镁合金表面的吸附强度和氧气气泡的大小，降低了微弧氧化陶瓷层孔隙率，提高了阳极氧化膜的致密性和耐蚀性。

After amplying a 2.2kb fragment form the PPV-SC1 RF-DNA,we clone the fragment into pMD 18-T,named pTNSl.The whole sequence which is 1989 bp long was determined by sequencing, including the complete ORF of PPV-SC1 NS1 which encoding 662 amino acids.Alignment of pairs of sequence indicates that there are 98% and 99% similary with other porcine parvovirus strains Kresse and NADL-2, respectively. Multiple sequence alignment discloses that there are a few difference between ppv-scl nsl gene and other ppv nsl gene: A-G at 39nt,T-C at 153nt,A-G at 175nt, A-C at 1117nt, A-C at 1535nt .Alternative codon in ppv-scl nsl have distinctly different frequentfy by codonbias analysis at EMBOSS(http://genopole.toulouse.inra.fr/bioinfo/emboss). Thereis not distinct hydrophobicity and transmenbrane helices in ppv-scl nsl protein. Struction domain anslysis of PPV-SC1 NS1 protein indicate that there are a ATP/GTP-binding site motif A at 398-405,16 Protein kinase C phosphorylation site,21 Casein kinase II phosphorylation site,and 3 cAMP/cGMP-dependent protein kinase phosphorylation site.At the same time ,there is a same motif between ppv-scl nsl and Poxvirus D5 protein-like which may share in the same fuction which is necessary during virion duplication.

将PPV-SC1 NS1序列与其他PPV NS1基因进行多序列比对，结果显示，PPV-SC1 NS1与其他的PPV NS1的同源性较高，仅存在个别的差异，分别是第39位A→G，第153位T→C，第175位A→G，第1117位A→C，第1535位A→C；同源搜索比较表明，PPV-SC1与PPV NS1同源性可达98％、99％，与其他的细小病毒NS1基因也存在很大的保守性；密码子偏向性分析结果表明PPV-SC1 NS1基因在同一氨基酸的不同密码子的选择上存在一定的偏向性；PPV-SC1 NS1蛋白总体上说具有亲水性不存在明显的疏水性区段，用swiss TMPRED软件预测PPV-SC1 NS1的跨膜区，返回的结果并没有得到有显著意义的跨膜区的存在；根据基于motif数据库的结构域预测，PPV-SC1 NS1的第393-415位氨基酸残基存在潜在的ATP／GTP结合位点，该蛋白还存在16个蛋白激酶C磷酸化位点，21个酪蛋白激酶2磷酸化位点，3个cAMP-／cGMP依赖蛋白激酶磷酸化位点，PPV-SC1 NS1蛋白与POX_D5(痘病毒D5蛋白)具有一致的保守结构域，推测NS1可能与POX_D5有类似的功能。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。