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An important intermediate of deoxyadenosylcobalamin biosynthesis by anaerobic pathway of Propionibacterium freudenreichii was separated from the broth in this paper.

在费氏丙酸杆菌(Propionibacterium freudenreichii)发酵生产维生素B12的研究中,分离到脱氧腺苷钴胺素合成途径中一种关键而稳定的中间产物。

The eluate was prepared by dissolving am- monium formate 6.3 g and octanen sulfoacid sodium 1.0 g in 700 mL water(pH 3.8 adjusted with formic acid)and subsequently diluted with water to 1000 mL.

结果:在本色谱条件下腺苷蛋氨酸与辅料、溶剂峰及其有关物质的分离度符合要求,在200~600μg·mL~(-1)范围内线性良好(r=0.9998)。回收率为99.61%~99.98%,RSD为0.23%~1.3%。

S-adenosylmethionine is a naturally occurring molecule, which widely distributes throughout tissues and fluids. It has been one of the very important pharmaceuticals because of its essential role in transmethylation, transsulphuration and transaminopropylation.

S-腺苷蛋氨酸是一种广泛存在于各种组织和细胞中的生物小分子,具有转甲基、转硫和转氨丙基等重要生理作用,已成为治疗肝损伤、胆汁郁积和抑郁症等疾病的重要药物。

RESULTS: Intracerebroventricular injection of SAM produced a significant decrease in blood pressure and heart rate of the anesthetized rats.

结果:侧脑室注射S-腺苷蛋氨酸能够显著降低麻醉大鼠的血压,减慢大鼠的心率。

The optimum condition for separating and purifying SAM was using the solution at pH of 5.0 containing 3.0 g SAM per liter at the flow velocity of 6 L/h through the ion-exchange column (φ50 mm×1500 mm)filled with D152 resin. Impurities were removed first by eluting the column with double-distilled water and acetic acid, and then with 9 L to 15 L of sulfuric acid of 50 mmol/L concentration at the flow velocity of 6 L/h.

论文对SAM的性质、药理作用以及当前国际上关于SAM的研究进展进行了较为详尽的综述,并在此基础上确立了研究内容,取得了如下成果: 1利用本实验室构建的基因工程菌JM71发酵生产S-腺苷-L-蛋氨酸,在50 L发酵罐中发酵6天后,SAM终产量达到6 g/L发酵液。

After inoculation of leukemia virus,the changes in ANAE,AcP and β-Gase reactions were observed in the thymocyte and thymie nurse cell in thymus cortex of experimental mice.

对接种白血病病毒后30d、75d 小鼠胸腺和淋巴结的组织结构及酸性非特异性酯酶、酸性磷酸酶、β~葡糖苷酸酶反应类型进行了观察,并与正常组作对照。

A co-regulation existed in the expression among chalcone synthase, methionine sulfoxide reductase and acyl carrier protein Ⅱ, also did in the expression between allergenic protein and prolamine.

查尔酮合酶、S-腺苷基甲硫氨酸还原酶和酰基载体蛋白Ⅱ的表达存在基因间共调控,过敏反应蛋白和醇溶蛋白基因的表达也存在基因间共调控。

Objective To study the changes of N-methyl-D-aspartate-receptor-channels current in developing hippocampal neurones during hypoxia and effect of adenosine intervention.

目的 研究缺氧对发育中的海马神经元N-甲基-D门冬氨酸受体通道电流的影响及腺苷干预作用的离子通道机制。

Aliquots were subjected to impedance aggregometryin response to collagen, adenosine diphosphate, ristocetin,and arachidonic acid performed at 22°C, 34°C, 37°C,and 40°C.

一部分用作在22°C 、34°C 、37°C 和40°C 下测定对胶原、二磷酸腺苷、瑞斯西丁素和花生四烯酸反应中的阻抗集合度测试。

Methods Platelet aggregations induced by ADP, AA and PAF, respectively, were measured by turbidimetric method after platelet incubated with 5'-nucleotidase.

采用比浊法测定白唇竹叶青蛇毒5'-核苷酸酶对二磷酸腺苷、花生四烯酸、血小板活化因子诱导血小板聚集的影响。

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The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作:对伸臂位于顶部的单层框架—筒体模型进行分析,提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。

Its advantage is that it can be used in smaller units.

其优点在于可以在较小的单位中应用。