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Using interproscan to analysis on homology to VP60B, a piece of sequence of amino acid in VP60B was found homology with adenovirus type 5 fiber protein knob domain.

VP60B的一段序列与腺病毒纤维蛋白(Adenovirus type 5 fiber protein)的knob domain的一段序列具有同源性。

Methods The tissues of cervical carcinoma with HPV16/18positivity was injected into the hypodermis of nude mice to make tumors and transmission,then Ad-p53gene was injected into the tumors to investigate the effect,while DDP,BLM and normal saline were injected into the tumors to as control groups.

将含HPV16/18型感染的宫颈鳞癌组织注入裸鼠皮下成瘤、传代,将以缺陷型腺病毒为载体的P53基因(Ad-P53)瘤内注射,同时用抗癌药DDP、BLM以及生理盐水作对照,观察P53基因治疗宫颈癌的有效性。

The results indicate that BMP7 will have important role in gene therapy of bone defect repairing.

本研究BMP7重组腺病毒的构建成功,为骨科疾病基因治疗的进一步研究提供了基础。

With the adenoviral vector we construct,the synthesis shRNA insert the mammalian cells,inducing the DNA silence effect,to research the role of S7 in the genaration and progression of tumor.

我们通过构建腺病毒基因载体,将构建合成的shRNA转入细胞,诱导对S7的基因沉默效应,从而研究S7对肿瘤细胞发生、发展的作用。

Although a single intracardial injection of an adenovirus containing the AT2R into male Sprague-Dawley rats did not affect the MAP, the effectiveness of an AT1R antagonist was greater in the AT2R-overexpressing animals compared with those expressing a control vector.7

虽然在雄性斯普拉-道来大鼠心内注射包含血管紧张素II受体血管紧张素II受体的腺病毒后没有观察到平均动脉压的上升,但是在血管紧张素II受体过度表达的个体血管紧张素I受体拮抗剂在血压调控中起到了更为有效的作用。

Objective: To investigate the effects and mechanism of adenovirus-bcl-xs gene on the inhibition of ascites tumor cells and survival rate of nude mice transplanted intraperitoneally with human ovarian carcinoma.

目的:研究重组腺病毒bcl-xs基因对卵巢癌细胞及其裸鼠移植腹水瘤的生长抑制作用和荷瘤裸鼠生存率的影响,同时探讨bcl-xs基因对于卵巢癌的作用机制。

Taken together, the survivin promoter appears to be a superior TSP for breastcancer targeting among them because it had high activity in established cell lines withlower variation and lower activity in liver.

总之,启动子CXCR4和Survivin在乳腺癌细胞系中具有特异的转录靶向性,腺病毒系统中启动子Survivin较CXCR4而言,在乳腺癌细胞中的变异小,而且在重要器官肝脏的活性仅为CXCR4启动子的1/87.46,提示其在乳腺癌基因治疗中具有更好的应用前景。

NSCs were isolated from embryoic 10 days C57/BL6 mouse telencephalon by mechanical separation and cultivated with DMEM/F12(1:1) medium added B27 and basic fibroblast growth factor, bFGF.

但是水迷宫实验逃避潜伏期对比示注射重组VIP腺病毒小鼠和对照组小鼠学习和记忆能力未见显著性差别。

When MOI was 100, expression was obtained at mRNA and protein level.

第三部分:重组腺病毒对不同表达细胞和血管环转染的体外研究。

However, when the MOI was larger than 100pfu/cell, the cell growth was inhibited by viral toxicity.

但当MOI大于100pfu/细胞时,腺病毒会对细胞的生长产生抑制作用。

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