腺病毒

Methods The target DNA sequence of yrdC was obtained from human spleen tissue by using RT-PCR,construct high titer recombinant adenovirus Ad-yrdC by using AdEasy adenovirus carrier system,detect yrdC protein expression by using Western blot and immunohistochemical method,set up control group and transfected Ad-Null group,and study the effects Ad-yrdC on BGC-823 cell line by drawing cell growth curve and MTT chromatometry.

方法用RT-PCR方法获取人脾脏组织yrdC基因序列，采用AdEasy腺病毒载体系统构建携带yrdC基因的高滴度重组腺病毒Ad-yrdC，将其转染胃癌BGC-823细胞，应用Western blot和免疫组织化学法检测yrdC蛋白表达，设对照组和转染Ad-Null组，并分别绘制细胞生长曲线和MTT比色法研究Ad-yrdC对BGC-823细胞的影响。

However, molecular conjugate vector containing adenovirus could enter cells via adenovirus receptor which exists on large range type of cell membrane, its cell targeting is affected.

然而，由于在不同组织细胞膜存在腺病毒受体，分子偶联载体可能通过腺病毒受体进入细胞内，致使基因转移靶向性受到影响。

Objective: In order to investigate whether the recombinant adeno-associated virus -mediated foreign genes, LacZ/hVEGF, could pass the blood brain barrier by intra-carotid artery delivery or not and to investigate whether the foreign genes could be expressed in vivo in ischemic brain of the focal embolic stroke rats or not, a high titer and completely adenovirus free recombinant adeno-associated virus vector was constructed and studied.

目的：为了检测腺相关病毒载体携带的人类血管内皮因子基因能否通过血脑屏障及能否在脑组织中表达其基因产物，我们构建了一种新型、高滴度、无腺病毒污染的rAAV携带的报告基因和hVEGF基因分别经颈动脉对自体血栓局部脑卒中大鼠进行了研究。

The study mentioned above indicated that in addition to anti-adenovirus effect, prior effect of the contained- Qingfei oral liquid serum was to adjust the function of the body and histiocyte. So it could treat the child with adenovirus pneumonia. This embodied the multitarget effect Chinese herbs on treating viral diseases.

以上研究表明，清肺口服液含药血清除了有抗腺病毒的作用外，更重要的在于调节机体和相应的组织细胞功能，从而达到治疗小儿腺病毒肺炎的目的，体现了中药治疗病毒性疾病作用的多靶点效应。

Objective: To find the way to inactivate the contaminated adenovirus in the air of clean room and laminar hood.

目的：寻找对腺病毒灭活的有效方法，来预防大规模生产腺病毒时所致的生产细胞的污染。

After linearisation by restriction enzyme PacI, the recombinant plasmids were transfected into HEK 293-cells and amplified to give large quantities of infectious recombinant adenovirus carrying the sequences of si-FOXO1s, designated Ad-si-FOXO1-1, Ad-si-FOXO1-2, Ad-si-FOXO1-3, Ad-si-FOXO-pan and Ad-NC, respectively.

人工合成si-FOXO1s的cDNA序列，退火形成的双链cDNA克隆到腺病毒表达载体pAdTrack-CMV上，经PmeI线性化后与pAdEasy-1细胞内同源重组，最后在HEK293细胞中包装为重组腺病毒，分别命名为Ad-si-FOXO1-1、Ad-si-FOXO1-2、Ad-si-FOXO1-3、Ad-si-FOXO-pan和Ad-NC。

Pocine interferon α mature protein genes were amplified by reverse transcription polymerase chain reaction, and the recombinant replication-defective human adenovius serotype 5 plasmid pAd-poIFN-α was constructed. When the recombinant plasmid pAd-poIFN-α was linearized with PacI, and then transferred into HEK-293A cells, the virus plaque was isolated and purified in HEK-293A cells by three of plaque purification passages.

本研究利用RT-PCR方法扩增猪α干扰素成熟蛋白基因后构建了重组腺病毒质粒pAd-poIFN-α，经PacI酶切后转染HEK-293A细胞，3次噬斑纯化后获得了重组腺病毒rAd-poIFN-α。

Reverse Transcription-Polymerase Chain Reaction was used to clone the whole-length gene of BMP-2 and construct AdV vector.

应用反转录-聚合酶链反应法克隆出骨形态发生蛋白质类2全长基因，构建重组腺病毒载体，DNA-磷酸钙共沉法将辅助质粒PJM17转染293细胞，同源重组构建出重组腺病毒。

The inhibition of PC3 ceils proliferation by AD-HOSM was determined by trypan blue exclusion ceil count assay.

结论重组腺病毒介导HOSM表达能有效抑制PC3在体外的增殖，提示重组腺病毒介导的HOSM基因治疗可能成为胰腺癌治疗的候选方案。

Methods RNAi combinant adenovirus vector which targeted 1566 site of NF-κB p65 mRNA was constructed and the effect of p65 gene knockdown in HUVEC was detected by Western blot analysis.

构建针对NF-κBp65 mRNA序列1566位点的重组RNAi腺病毒表达载体，并利用RNAi腺病毒抑制HUVEC p65表达。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。