腺病毒

Secreted by the epithetical cells of animal thymus, Ta1 is widely distributed. It is an immunoregulatory peptide with multiple biological activities such as modulate cell-mediated immunity, anti-aging and anti- virus activity and so on.

它由动物胸腺上皮细胞分泌，在体内分布广泛，是一具有多种生物学活性的免疫调节剂，可调节细胞免疫功能，有较好的抗衰老和抗病毒作用等。

Based on relative literatures,this part has included five resesearch aspects as below.1 Fifteen compounds were isolated and purified by extraction,column chromatography,and their structures were determined on the basis of spectral analysis: acteoside(Ⅰ-1),isoacteoside(Ⅰ-2),crenatoside(Ⅰ-3),cistanoside F(Ⅰ-4),sinapoyl-4-O-β-D-glucoside(Ⅰ-5),adenosine(Ⅰ-6),β-siterol(Ⅰ-7),oleanic acid(Ⅰ-8), succinic acid(Ⅰ-9),caffeic acid(Ⅰ-10),protocatechuic aldehyde(Ⅰ-11),p-hydro xybenzyl alcohol(Ⅰ-12),β-daucosterol(Ⅰ-13),D-galacitol(Ⅰ-14),D-mannitol(Ⅰ-15).Ⅰ-4～15 were obtained from this plant for the first time,andⅠ-6,7,9,and 13 were isolated from Orobanche genus for the first time.2 The scavenging test of DPPH showed that most compounds have comparative antioxidant activity as L-ascorbic acid and part of them show better activity such as the O.coerulescens extract and phenylethanoid glycosides.Acteoside showed potent free radical scavenging effects with a median inhibition concentration of 25.6μg/ml.3 The anti-HBV activities of acteoside,isoacteoside and crenatoside were measured,and all of them showed suppressive activity on the expression of HBsAg and HBeAg in the HepG2.2.15 cell line.

本论文在文献调研基础上对紫花列当化学成分及生物活性进行了研究，并从免疫抗病毒角度探讨紫花列当中特征性成分类叶升麻苷的肝保护作用及其机制。1采用大孔树脂、硅胶和Sephadex LH-20等色谱技术对紫花列当进行系统的植物化学研究，从中分离得到19个化合物，利用UV和NMR等波谱手段及理化性质鉴定了其中的15个化合物，分别为类叶升麻苷(Ⅰ-1)、异类叶升麻苷(Ⅰ-2)、crenatoside(Ⅰ-3)、cistanoside F(Ⅰ-4)、sinapoyl-4-O-β-D-glucoside(Ⅰ-5)、腺苷(Ⅰ-6)、β-谷甾醇(Ⅰ-7)、齐墩果酸(Ⅰ-8)、琥珀酸(Ⅰ-9)、咖啡酸(Ⅰ-10)、原儿茶醛(Ⅰ-11)、对羟甲基苯甲酸(Ⅰ-12)、β-胡萝卜苷(Ⅰ-13)、D-半乳糖醇(Ⅰ-14)和甘露醇(Ⅰ-15除化合物Ⅰ-1、Ⅰ-2和Ⅰ-3外，其余化合物均为首次从该植物中分离得到。2)通过大孔树脂富集该药材有效部位苯乙醇总苷，并采用HPLC测得其所含特征性成分类叶升麻苷的含量可达80%以上。3DPPH自由基清除试验显示紫花列当提取物及其所含的苯乙醇苷类化合物均具有较好的抗氧化能力，其清除DPPH自由基能力接近于抗坏血酸。4采用卡介苗整体致敏、脂多糖离体攻击构建大鼠原代肝细胞免疫性损伤模型，在体外观察类叶升麻苷的保肝作用。

Adeno-associated virus;P27;MG-63 cell;Pien Tze Huang; osteosarcoma

腺相关病毒； P27 ； MG-63细胞；片仔癀；骨肉瘤

Three infectious bronchitis virus isolates named as Q1, J2 and T3 were isolated from proventricular tissue taken from vaccinated chicken flocks in China The biological characteristics of the three isolates were observed.

从我国接种过传染性支气管炎疫苗的幼鸡群的腺胃组织中分离得到三个传染性支气管炎毒株Q1、J2和T3，并对它们的部分生物学特性进行研究。将病毒通过9-11日龄鸡胚，盲传5-6代。

It is in urgent need of excogitating new HBV vaccines and searching for effective therapeutic methods since there is no panacea for chronic HBV infection to date.

迄今为止，慢性乙型肝炎的治疗仍无良策，因此，研制新型HBV疫苗和寻求有效的治疗乙型肝炎的手段迫在眉睫。2型重组腺相关病毒(adeno-associated vires type 2，rAAV-2)作为一种大有希望的基因转移载体，因能有效转导多种组织和细胞而引起了人们的高度重视。

Dr. Swanson is married to the chief executive officer of a gene therapy company that has optioned a license for adeno-associated virus–mediated correction of myotonic dystrophy.

Cooper医师报告没有相关财金关系，Swanson医师与一基因治疗公司的执行总裁结婚，该公司拥有腺相关病毒校正肌肉强直症之执照。

Methods:Anti-rubella virus actions of synthesized adenine and uridine derivants and ethanol extract of rhubarb were studied in three kinds of cell cultures. CPE was used as observation index in BHK21 cell culture.

选用9种新合成的腺嘌呤衍生物、6种尿嘧啶衍生物及大黄醇提液，分别在BHK21、RNC和HNC细胞上进行了体外抗病毒试验。

In vivo gene therapy of ovarian cancer by adenovirus－mediated thymidine kinase gene transduction and ganciclovir administration.

羟甲基无环鸟苷对携有单纯疱疹病毒胸腺嘧啶核苷激酶基因人卵巢癌裸鼠皮下移植瘤生长的抑制作用。

Bioluminescent imaging of AAV-transfected syngeneic grafts revealed gradual and stable transgene expression in graft cardiomyocytes.

生物发光成像显示腺相关病毒转染的同基因移植物在移植心肌中产生持续而稳定的表达。

After inoculation of leukemia virus,the changes in ANAE,AcP and β-Gase reactions were observed in the thymocyte and thymie nurse cell in thymus cortex of experimental mice.

对接种白血病病毒后30d、75d 小鼠胸腺和淋巴结的组织结构及酸性非特异性酯酶、酸性磷酸酶、β～葡糖苷酸酶反应类型进行了观察，并与正常组作对照。

I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时，滚道或滚动元件褪色是很常见的特征。