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腺病毒

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With in situ DNA hybridization, the EBV genomes have been demonstrated in the labial glands of the Sjogren′s syndrome patients (16/28), moreover the elevated content of EBV DNA has been identified in those with more severe destruction of labial glands. It is concluded that EBV, vhich has a normal site of latency at salivary glands in a nonpathogenic state, may be reactivated in Sjogren′s syndrome patients and play a role in the pathogenesis of this disease.

另外,我们用原位杂交方法观察了EB病毒DNA在干燥综合征病人唇腺组织的存在状况,结果发现28例干燥综合征病人中16例为阳性,其中腺泡组织破坏严重者,EB病毒DNA的含量亦高,并且EB病毒主要分布于大量浸润的淋巴细胞中,其次位于腺泡上皮细胞内,少量见于腺泡腔中,以上结果提示,在干燥综合征病人EB病毒处于活跃的复制状态,干燥综合征病人靶器官受损与EB病毒的活跃复制及其诱导的免疫反应密切相关。

Objective:in order to compare the curative effects of using lamirudine and thymosin together with using thymosin only to the paients of chronic hepatitis b.methods:we had chosen 27 medical cases of chronic hepatitis b,which were divided into two groups:15 cases asone group,and 12 cases as the other group.let group a take 100 mglamirudine orally,qd,with half a year as a period of treatment.at the same time givethe patients injections of 100 mg thymosin,qd,for a month.to group b was only takenlamirudine.alt,tbil,the indicators of the virosis of hepatitis b (hbsag,hbeag,hbv-dna)were observed as quatos.results: at the end of the treatment, the rates that groupa and group b made hbsag transform into negative were respectively 26.67% and 16.67%(p>0.05);hbeag:33.33%,8.33%;hbv-dna:53.33%,33.33%(p>0.05).conclusion:combining thymic peptide with 3tc to treat chronic type b hepatitis, thymic peptide will not only promote b-cell to mature and transformation, but also to keep the subpopulation of t-cell in immune system in balance, which can enhance the reaction of matured t-cell to the antigen or other stimulus.

目的:比较拉米夫定(3tc)与胸腺肽联用和单用拉米夫定治疗慢性乙型肝炎的疗效。方法:选择慢性乙型肝炎患者27例,分为联合组15例和拉米夫定组12例;联合组用拉米夫定100 mg,口服,qd,疗程半年,合并用胸腺肽100 mg,静滴,qd,疗程1个月。拉米夫定组单用拉米夫定。以谷丙转氨酶、血清胆红素、乙肝病毒标志物(hbsag,hbeag,hbv-dna)为观察指标,比较不同治疗方案对指标的影响。结果:治疗结束时联合组与拉米夫定组对慢性乙肝患者血清hbsag阴转率分别是26.67%和16.67%(p>0.05);hbeag阴转率分别是33.33%和8.33%(p>0.05);hbv-dna阴转率分别是53.33%和33.33%(p>0.05)。结论:胸腺肽联合3tc治疗慢乙肝,胸腺肽具有促进t淋巴细胞的成熟和转化,并维持免疫系统各t淋巴细胞亚群间的平衡作用,能增强成熟t淋巴细胞对抗原或其他刺激的反应。

Four viral strains were found in proventricular tissues; three of them were identified as reticuloendotheliosis virus, coronavirus and Newcastle disease virus respectively. Another unidentified virus was of spherical shape, ranging from 60nm to 80nm in diameter, and could be found only among cells.

从腺胃组织的超薄切片中可观察到四种病毒颗粒,分别为IBV、网状内皮组织增生症病毒和新城疫病毒,此外还有一种呈球形,大小在60~80nm的病毒颗粒,该病毒主要分布于细胞间隙中,尚未鉴定。

The remaining eighteen pigs, served as three viral administered groups, underwent coronary artery ligation and received intramyocardial viral injection. rAAV- GFP group (n=6), rAAV-CD151 group (n=6) and rAAV-antiCD151 group (n=6) animals respectively received direct intra- myocardial injection of rAAV-GFP, rAAV-CD151 and rAAV-antiCD151 1×10~(12 viron particles per pig, at 10 sites correspondingly.

采用三质粒共转染法包装CD151、antiCD151和GFP重组腺相关病毒(recombinant adeno-associated virus,rAAV)。2.1月龄小型猪22头,随机分为4组,分别为正常对照组4头(不予冠脉结扎和注射病毒,正常喂养),rAAV-GFP组6头结扎冠状动脉左前降支,心肌内分10点注射1×10~(12viron particles的rAAV-GFP病毒,rAAV-CD151组6头结扎冠状动脉左前降支,心肌内分10点注射1×10~(12viron particles的rAAV-CD151病毒,rAAV-antiCD151组6头结扎冠状动脉左前降支,心肌内分10点注射1×10~(12vironparticles的rAAV-antiCD151病毒。

The compositions of fatty acids in mid-gut gland of cultured healthy Penaeus monodon, healthy Penaeus japonicus, P. monodon slightly infected by MBV, P. monodon severely infected by MBV and P.

应用气相色谱法分别检测了人工养殖的健康斑节对虾、健康日本对虾、斑节对虾杆状病毒轻度感染斑节对虾、MBV重度感染斑节对虾及患中肠腺坏死杆状病毒病日本对虾中肠腺脂肪酸的组成及含量。

Consequently E1B deletion adenovirus had been approved to take clinical trial by China State Food and Drugs Administration.

因此E1B缺失重组腺病毒被国家药品食品管理局批准进行人体试验研究。

A recombinant adenoviral vector encoding PKG Ⅱ was successfully constructed and primary result of experiment with the vector confirmed that PKG Ⅱ could inhibit EGF induced MAPK signaling. This provided the foundation for further research about the role of PKG Ⅱ in tumorigenesis.

成功构建了携带PKG Ⅱ基因的重组腺病毒,初步证明PKG Ⅱ对细胞增殖相关信号转导有抑制作用,为进一步研究PKG Ⅱ与肿瘤细胞发生发展的关系提供了基础。

Objective To investigate the role of SOX-9(SRY-related high mobility group box gene 9)regulating the apoptosis in the hyaline cartilage cells of human cervical zygapophysial joints.

目的 探讨重组腺病毒介导的人SOX-9(AdSOX-9)对人的颈椎关节突关节软骨细胞凋亡细胞数目及Caspase-3基因表达的作用及相关意义,更好理解颈椎软骨退变的发生机制。

Adenovirus-mediated HSV-TK/GCV system can effectively prevent PCO. However, it has toxicity for iris and ciliary body.

腺病毒载体介导的HSV-TK/GCV系统能够有效抑制PCO,但对虹膜睫状体有一定的毒副作用。

Objective To explore the appropriate method of isolating, purifying and culturing the rat bone marrow mesenchymal stem cells in vitro, and observe the differentiation of MSCs into neuron-like cells induced by noggin gene transfected with adenovi- rus vector.

目的 探讨体外分离、培养大鼠间充质干细胞的方法,采用重组腺病毒介导的noggin基因体外诱导方法,观察 MSCs向神经细胞的定向分化效应。

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The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作:对伸臂位于顶部的单层框架—筒体模型进行分析,提出了等效转动约束的概念和转动约束刚度的表达式。

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