腺病毒

Methods: The telomerase activity in breast cancer cells and MRC-5 cell line were detected semi-quantitively by TRAP-ELISA assay. Virus proliferation as- say and cell viability assay were used to evaluate the proliferation and cytolysis selectivity of CNHK300, and the results were compared with those of wtAd5. The breast cancer cells were treated with CNHK300 combining paclitaxel to evaluate the killing effect of the combined regimen.

用TRAP-ELISA方法检测乳腺癌细胞株(MDA-MB-453、SK-BR-3)和正常成纤维细胞株MRC-5的端粒酶活性；进行病毒增殖实验和细胞生长抑制实验，并与野生型腺病毒wtAd5进行对比，验证CNHK300选择性复制和选择性杀伤能力；用细胞生长抑制实验进一步考察CNHK300与化疗药物紫杉醇联合应用对乳腺癌细胞的杀伤作用；用Western blot检测腺病毒E1A在细胞中的表达。

AIM: To detect the stability of the recombinant adenovirus plasmid containing major outer membrane protein gene from Chlamydia psittaci in HEK293 cells, to evaluate whether cross reactivity exists or not between recombinant adenovirus and positive serum of Egg Drop Symdrome, and to evaluate whether SPF chicks vaccinated with the recombinant adenovirus were protected or not when challenged with virulent CpL strain.

目的： 检测重组腺病毒质粒在HEK293细胞中的稳定性；重组腺病毒是否与减蛋综合征阳性血清发生中和反应，以及用重组腺病毒免疫SPF小鸡是否产生保护。

Results The concentration of Chlorogenic acid was 0.05mg/ml, 0.1mg/ml, 0.4mg/ml, 0.8mg/ml respectively, it can respectively inhibit respiratory syncytia virus, Coxsackie B3 virus, adeno-associated 7 virus, adeno-associated 3 virus and Coxsackie B5 virus.

结果 氯原酸浓度分别为0.05mg/ml、0.1mg/ml、0.4mg/ml、0.8mg/ml时，可分别体外抑制合胞病毒、柯萨奇B3、腺病毒7型、腺病毒3型和柯萨奇B5型。

Results The concentration of Chlorogenic acid was 0.05mg/ml, 0.1 mg/ml,0.4mg/ml,0.8mg/ml respectively,it can respectively inhibit respiratory syncytia virus,Coxsackie B 3 virus,adeno associated 7 virus,adeno associated 3 virus and Coxsackie B 5 vi...

结果 氯原酸浓度分别为 0 。0 5mg/ml、0 。1mg/ml、0 。4mg/ml、0 。8mg/ml时，可分别体外抑制合胞病毒、柯萨奇B3、腺病毒 7型、腺病毒 3型和柯萨奇B5型。结论氯原酸对常见呼吸道病毒有较强的抑制作用

Moreover, overpression of Wnt10b in the organ culture of vibrissae HF has been manipulated by infected the recombinant adenovirus expressed Wnt10b protein(Adwnt10b).

Ki67在毛母质中的表达变化规律以及在毛母质中的表达部位与Wnt10b具有一致性。2、我们对Wnt10b重组腺病毒进行了逐级扩增并应用氯化铯超高速密度梯度离心法纯化，病毒最终滴度达到109 PFU/ml；3、首次发现加入Wnt10b重组腺病毒可明显促进体外器官培养的触须毛囊毛干和内根鞘生长，这与本课题组在在体动物实验中发现的现象一致。

Methods Recombinant adenovirus GDF-5 (Ad-GDF-5) containing green fluorescent protein and Ad-GFP were amplifiedand tittered. hBMSCs at passage 3 were infected with two viruses at different titers. At 2 days after intervention, GFP expression was observed using fluorescence microscope, and GDF-5 expression in hBMSCs was detected by RT-PCR.

将重组腺病毒GDF-5（adenovirus GDF-5，Ad-GDF-5）和空载腺病毒Ad-GFP 进行扩增并测定滴度，并以不同滴度两种病毒感染第3 代hBMSCs，干预后2 d 荧光显微镜下观察GFP 表达情况，RT-PCR 检测GDF-5 在hBMSCs中的表达。

Methods The serum of 187 children with pneumonia from July 2002 to December 2004 in hospital were investigated for respiratory syncytial virus, adenovirus 3 influenza viruses A and B, parainfluenza virusestype 1,2,3,4 and coxsackievirus A 1.7, echovirus 7 by employing the indirect immunofluorescence assayfor the identification of nearly 8 different viruses, and 3 different enteroviruses.

采集2002年7月~2004年12月因肺炎住我院儿科的187例患儿血清，应用间接免疫荧光法检测8种常见呼吸道病毒病原体和3种肠道病毒的抗体：呼吸道合胞病毒、腺病毒3(ADV3)、流感病毒A和B型、副流感病毒l~4型、柯萨奇病毒A1、7型、埃可病毒7型的特异型IgM抗体。

Results The highest positive rates were found in respiratory syncytial virus (RSV,30.5%) and adenovirus (AdV,27.4%),and the other findings were as following: Coxsackie virus (CV,11.3%), EB virus (EBV,6.5%),echovirus (ECHOV,4.8%),parainfluenza virus (PIV,1.6%), influenza virus B (IVB,1.6%) and influenza virus A (IVA,0%).

结果 呼吸道合胞病毒、腺病毒阳性率最高，分别为30.5%、27.4%，其次为柯萨奇病毒、EB病毒，分别为11.3%、6.5%，埃可病毒为4.8%，副流感病毒及流感病毒B均为1.6%，流感病毒A为0%。

In vitro killing effect of adenovirus - mediated herpes simplex virus thymi- dine kinase gene under regulation of hypoxic response element on hepatoma cell line HepG2WANG Xiao - zhong, PENG Qi - quan, PENG Yun - heng, LIAO Wen - peng Department of Hepatobiliary Surgery, Central Hospital of Shantou City, Shantou 515031, China.

王小忠 彭启全彭云恒廖文鹏广东省汕头市中心医院肝胆外科，广东汕头515031摘要：目的：构建携带受低氧反应元件调控的单纯疱疹病毒胸苷激酶基因的重组腺病毒载体，探讨该重组腺病毒对体外培养的肝癌细胞HepG2的特异性杀伤活性。

The biological activity of IFN-γwas detected in the supernatant of the cultured 293 cells following rAds' infection, and it could be blocked by the antibody for IFNγ. The B16F10 cells infected with the purified rad (RSV/IFN-01) didn't show cytopathic effect , and more biological activity of IFN-γwas detected in the infected B16F10 cells' supernatant with the increased MOI values. Therefore, the recombinant adenovirus constructed in this study can express and secrete human IFN-γ.

其中的1号重组病毒纯化后，按不同的MOI值感染复制缺陷型腺病毒不能在其中增殖的B16F10细胞，未出现细胞病变效应（cytopathic effect，CPE），而上清中的γ-干扰素活性却随MOI值增大而升高：这证实构建的复制缺陷型腺病毒是能表达并分泌人γ-干扰素的。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。