腺病毒

The recombination vector was digested by Pac I enzyme and transfected into 293A cell by Lipofectamine method to obtain recomb...

该载体经PacI酶切，脂质体法转染人胚肾293A细胞，获得重组腺病毒载体pAD-E7.pAD-E7转染人HaCaT细胞，激光共聚焦显微镜分析E7蛋白表达情况。

Methods: We constructed a recomb ined adenoviral vector carrying CD or LacZ. 293 packaging cell was trans fected by the newly-constructed plasmid and generated the infectious virus.

构建含CD或LacZ基因的重组腺病毒载体，在293细胞内重组包装后，体外感染小鼠结肠癌细胞株C26，并给予不同浓度的前药氟胞嘧啶(5-FC)进行肿瘤杀伤效果观察。

A RU486 regulatable replication-defective recombinant adenoviruses vector containing single chain fusion murine IL-12 gene was successfully constructed,which provides a good basis for further research on gene therapy in vivo.

成功构建了重组腺病毒 Ad-RUmIL-12，可以通过RU486诱导mIL-12单链融合蛋白的表达，为下一步体内基因治疗奠定了良好的基础。

OBJECTIVE: To construct a novel adenoviral eukaryotic expression vector that can co-express mutant hypoxia-inducible factor-1 alpha (HIF-1α) target protein and humanized Renilla reniformis green fluorescent protein reporter molecule under normoxic conditions.

目的：构建能够同时表达突变型低氧诱导因子1α(hypoxia inducible factor 1 alpha，HIF-1α)目的蛋白和人源化绿色荧光蛋白(human renilla reniformis green fluorescent protein，hrGFP)报告分子的新型腺病毒真核细胞表达载体。

Objective To investigate the effect of adenovirus-mediated gene therapy with vascular endothelial growth factorand basic fibroblast growth factordelivered into the subdermal space to treat compromised skin flaps in rats.

目的探讨以腺病毒载体局部应用血管内皮生长因子和碱性成纤维细胞生长因子基因治疗对大鼠缺血皮瓣生存的影响。

Methods The hTERT promoter as E1A and E1Bp gene were amplified by PCR; the GFP gene and GM-CSF were also amplified by PCR, and the products were subcloned into Teasy plasmid in certain order to generate pSh-GFP-SV55K plasmid.

采用PCR的方法克隆腺病毒E1区基因(E1A， E1B55K)、EIB的启动子(E1Bp)以及人端粒酶逆转录酶亚基启动子，以及2个目的基因：人GM-CSF和GFP(Green Fluorescence Protein， GFP)。

The proliferation of cultured rat renal fibroblasts was measured by means of methylthio tetrazole method.

用重组PTEN腺病毒转染体外培养大鼠肾成纤维细胞，MTT法检测细胞增殖活力，免疫细胞化学检测PCNA蛋白表达。

Objective: To study the inhibitory effects on gastric cancer by adenoviral transduction of mouse endostatin gene.

目的：研究腺病毒介导的鼠内皮抑素基因对胃癌的治疗作用。

ABSTRACT: Objective In order to investigate the treatment of bone defect repairing by recombinant adenovirus transudation the hBMP7 gene, the vector of hPMP7 recombinant Ad was constructed.

目的 为了研究人骨形态发生蛋白7(hBMP7)导入骨缺损模型中的治疗作用，构建重组腺病毒载体。

Considering undividing cells might be transfected with adenovirus vector andmacrophages could be activated by IL-2,we transfected mIL-2 gene into the freshlyisolated peritoneal macrophages with recombinant adenovirus,and pulsed with tumorantigen in vitro,in order to enhance the immune effector function as well as theantigen presentation function simutaneously,and acquired more effective actions ofantitumor.

本研究选用可转染非分裂细胞的缺陷型腺病毒为载体，将活化巨噬细胞的小鼠IL-2基因转染至小鼠腹腔巨噬细胞，并在体外行肿瘤抗原冲击，以期在增强其免疫效应功能的同时充分发挥其抗原提呈功能，从而取得更好的抗瘤效果。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。