腺病毒

The model of HCC was established by injecting subcutaneously the cells in different groups in nude mice.

结果 重组腺病毒AdEasy-GFP-ASmrp滴度可达2.5×10〓efu／ml，MOI为100时，即可致90％以上的肝癌细胞SMMC-7721／ADM被感染。

The infection efficiency of adenovirus was determined by the expression level of CAR and integrin on cancer cell surface, lack of CAR exression is associated with poor infectivity.

肿瘤细胞表面CAR和integrin的表达水平与5型腺病毒感染细胞的效率呈正相关。

The titre of purified Adenovirus vector was up to 10〓pfu/ml after packaging and expanding in 293 cells.

重组腺病毒载体在293细胞中包装后可以得到滴度高达10〓pfu／ml的病毒液。

Objective To investigate the effect of adenoviral vector infection on the differentiation potential of human bone marrow mesenchymal stem cells.

目的 研究腺病毒感染骨髓间充质干细胞对MSC分化潜能的影响。

The constructed anenterous adenovirus carrier may be used in developing anticancer medicine for treating tumor.

本发明构建的无肠腺病毒载体，可以开发出有效的治疗肿瘤的抗癌新药。

The present invention constructs anenterous adenovirus carrying trans-activator expression cassette and anticancer gene expression cassette.

本发明构建了携带有反式作用元件(trans-activator，TA)表达盒以及抗癌基因表达盒的无肠腺病毒或AAV。

Methods The skeletal muscle stem cells were enzymatically isolated with collagen and dispase, and then purified by different adhesion time from 6 female SD rats. The purified cells were transinfected with adenovirus carrying lac-Z gene, and injected into the bladder neck of 6 autologous and 4 allogeneic rats. Tissues of blader neck and ureter were obtained in sacrified rats after 5 and 15 days, histological examination and X-gal staining were performed.

采用混合酶消化及反复差速贴壁法分离、纯化培养SD大鼠骨骼肌干细胞，然后用携带lac-Z基因的腺病毒载体转染肌干细胞，待转染成功后将携带lac-Z基因的骨骼肌干细胞注射于自体和同种异体的膀胱颈及后尿道周围，分别于注射后5、15d，处死大鼠取出膀胱颈及后尿道，行组织学检查及X-gal染色。

These results suggest that the chimeric oncolytic adenovirus H101-F35 vector might be a useful candidate for gene therapy of cancer.

这些结果表明嵌合型溶瘤腺病毒载体H101-F35在肿瘤基因治疗中将有很好的应用前景。

An expression vector for one isoform of the mbC α1 subunit cDNA was constructed using the human cytomegalovirus promoter to direct expression and this expression vector was used in a novel transfection assay of primary cultures of bovine adrenal chromaffin cells.

为表达具有生物活性的钙离子通道，将mbC的一个异构体的蛋白编码区插入到由人的涎腺病毒启动子控制表达的质粒中。这个表达质粒

Methods: Ana lyse the express ion of p53 gene and inhibited growth of QBC939 cell lines by combination applica tion p53 gene mediated by recombination adenoviral vector and cisplatine.

将重组体腺病毒p53和顺铂联合作用于人胆管癌细胞QBC939，对p53基因的表达、细胞的生长抑制及机制进行分析。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。