腺嘌呤基

DNA consists of a chain made from four types of nucleotide subunits: adenine, cytosine, guanine, and thymine.

的DNA链组成一个由4种核苷酸亚基：腺嘌呤，胞嘧啶，鸟嘌呤和胸腺嘧啶。

The basic group has five kinds, respectively be adenine, guanine, cytosine, thymine and uracil.

碱基有五种，分别为腺嘌呤、鸟嘌呤、胞嘧啶、胸腺嘧啶和尿嘧啶。

There are four bases, called adenine, thymine, guanine, and cytosine.

共有四个碱基：腺嘌呤、胸腺嘧啶、鸟嘌呤和胞嘧啶。

After thecomplete genome extraction of the strain was performed, the genomic DNA was partiallydigested by restriction enzyme Sau3AⅠ, the DNA fragments from 1 to 5Kb was clonedinto prokaryote expression vector pET-28a-c, and transformed host bacteria. The resultsshowed that we succeeded in constructing the gene expression library of haemophilusparasuis serovar 5, which is fundamental for the study of advanced gene screening. Inaddition, primer design was performed based on haemophilus influenzae in this study. In addition, PCR was performed by using genomic DNA of haemophilus parasuisserovar 5 as the template. The results demonstrated that we obtained two neo-gene:23SrRNA gene(conserved gene belonging to the large-subunit of ribosome) and adenylatecyclase gene(encodes adenylate cyclase and participates in converting adenyl nucleosidetriphosphate to cyclic adenosine3",5"-monophosphate). Furthermore, the phylogeneticanalyses between the species was performed, and neighbor-joining tree was constructedbased on comparison of 23S rRNA gene sequences, so it was illuminated betweenHaemophilus parasuis and other species in molecular evolution relationship.

选择我国流行优势菌株副猪嗜血杆菌血清5型地方株为研究对象，提取细菌基因组DNA，用限制性内切酶Sau3AⅠ对基因组DNA进行部分酶切，回收大小为1～5Kb的DNA片段，将其连接入原核表达载体pET-28a-c，最后转化宿主菌，结果成功地构建了基因表达文库，为后续的基因筛选工作奠定基础；另外，本研究选择嗜血杆菌属的流感嗜血杆菌为参考对象进行引物的设计，以副猪嗜血杆菌血清5型地方菌株的基因组DNA为模板，进行PCR扩增反应，结果表明成功地获得两个新基因：23S rRNA基因(存在于核糖体大亚基中的保守性基因)和腺苷酸环化酶基因(负责将腺嘌呤核苷三磷酸转变为环腺苷酸)，并进一步做了不同物种之间的分子系统发育分析，构建了基于23S rRNA基因的邻接法系统发育树，阐明了副猪嗜血杆菌与其它菌种的分子进化关系。

These compounds were identified as follows:chrysophanol (FP-1),physcion(FP-2),eriosematin(FP-3),scoparone(FP-4),lupeol(FP-5),betulinic acid(FP-6),3\',4\'-Dihydroxy-trans-cinamic acid octacosyl ester(FP-7),β-sitosterol (FP-8),flemiphilippinone A(FP-9),monopalmitin(FP-10),emodin(FP-11),islandicin (FP-12),salicylic acid(FP-13),p-methoxyphenylpropionic acid(FP-14),trideca-1, 3-diene(FP-15),lupinifolin(FP-16),flemichin D(FP-17),flemiphilippinin A(FP-18), auriculasin(FP-19),erythrinin B(FP-20),6-C-prenylluteolin(FP-21), 8-(1,1-dimethylallyl) genistein(FP-22),flemiphilippinin E(FP-23),flemiphilippinin F (FP-24),5,7,3\',4\'-tetrahydroxy-6,8-diprenylisoflavone(FP-25),flemiphilippinin D (FP-26),dorsmaninsⅠ(FP-27),osajin(FP-28),6,8-diprenyleriodictyol(FP-29), lupinalbin A(FP-30),genistein(FP-31),3\'-O-methylorobol(FP-32),orobol(FP-33), 5,7,2\',3\',4\'-pentahrdroxyflavone(FP-34),the mixture of biochanin A and prunetin (FP-35 and 36),genistin(FP-37),sophororicoside(FP-38),3\'-O-methylorobol-7-glucoside(FP-39),the mixture of sissotrin and prunetin 4\'-O-β-D-glucoside(FP-40 and 41),adenosine(FP-42) and luteoloside(FP-43,mixture).

这些化合物分别为大黄酚(FP-1)、大黄素甲醚(FP-2)、eriosematin(FP-3)、滨蒿内酯(FP-4)、羽扇豆醇(FP-5)、白桦酸(FP-6)、咖啡酸二十八烷酯(FP-7)、β-谷甾醇(FP-8)、蔓性千斤拔酮A(FP-9)、单棕榈酸甘油酯(FP-10)、大黄素(FP-11)、islandicin(FP-12)、水杨酸(FP-13)、对甲氧基苯丙酸(FP-14)、十三烷-1，4-二烯烃(FP-15)、lupinifolin(FP-16)、千斤拔素D(FP-17)、蔓性千斤拔素A(FP-18)、auriculasin(FP-19)、erythrinin B(FP-20)、6-C-异戊烯基木犀草素(FP-21)、8-(1，1-二甲烯丙基)-染料木黄酮(FP-22)、蔓性千斤拔素E(FP-23)、蔓性千斤拔素F(FP-24)、5，7，3′，4′-四羟基-6，8-双异戊烯基异黄酮(FP-25)、蔓性千斤拔素D(FP-26)、dorsmaninsⅠ(FP-27)、osajin(FP-28)、6，8-双异戊烯基圣草素(FP-29)、lupinalbin A(FP-30)、染料木黄酮(FP-31)、3\'-O-甲基香豌豆苷元(FP-32)、奥洛醇(FP-33)、5，7，2′，3′，4′-五羟基黄酮(FP-34)、鹰嘴豆素甲和樱黄素的混合物(FP-35和FP-36)、染料木苷(FP-37)、槐属苷(FP-38)、7-葡萄糖基-3\'-O-甲基香豌豆苷(FP-39)、印度黄檀苷和樱黄素4′-O-β-D-葡萄糖苷的混合物(FP-40和FP-41)、腺嘌呤核苷(FP-42)和木犀草苷(FP-43，混合物)。

Moreover, the preliminary test of the biological activity of the 8 target derivatives showed that 7-(2-adenine)ethoxyl-2-ethyl-4"- methoxy isoflavone and 7-(2-hydroxyl-3-piperidino)propoxy-2-ethyl-4"- methoxyl isoflavone have fine biological activities on suppression of the growth of MCF-7 cell in breast cancer and the hela cell in cervical cancer of human being.

另外还对8个目标衍生物进行了初步的药理活性试验，发现7-(2-腺嘌呤)乙氧基-2-乙基-4′-甲氧基异黄酮和7-(2-羟基-3-哌啶)丙氧基-2-乙基-4′-甲氧基异黄酮均有较好的抑制人乳腺癌MCF-7细胞和人宫颈癌hela细胞生长的药理活性。

Thermally denatured DNA was preconcentrated at pretreated GCE by its adsorption in open circuit for 5min or at the potential of+0.3V for 90s, and produced two well-defined oxidation peaks of guanine and adenine residues at+0.80V and+1. 11V in pH 5. 0 phospoate buffer, respectively, but the native DNA did not produce any peaks at the same conditions.

在pH 5.0的磷酸盐缓冲液中，于+0.3V富集90秒或在开路条件下吸附富集5分钟，热变性DNA可以吸附富集在预处理玻碳电极表面，阳极扫描时，鸟嘌呤和腺嘌呤残基产生两个很好的氧化峰，峰电位分别为+0.80V和+1.11V，而在同样条件下，天然DNA却几乎不出现峰。

They were two new compounds, [cyclo(Pro-Val-Phe-Phe-Pro-Val-Phe-Ser-Leu),7],[2-N-(1-methoxycarbonylethyl)guanosine, 10], and eight known compounds,(p-hydroxybenzoic acid, 1),(methyl succinate, 2),(russulaceramide, 3),(phenylalanine, 4),(guanine, 5),(β-carboline, 6),(uridine, 8), and (adenosine, 9). All the compounds were isolated from Amanita exitialis for the first time.

分别为：对羟基苯甲酸(p-hydroxybenzoic acid，1)、丁二酸二甲酯(methyl succinate，2)、神经酰胺(russulaceramide，3)、苯丙氨酸（phenylalanine，4）、鸟嘌呤核苷(guanine，5)、β-咔啉(β-carboline，6)、环（脯氨酸-缬氨酸-苯丙氨酸-苯丙氨酸-脯氨酸-缬氨酸-苯丙氨酸-丝氨酸-亮氨酸） [cyclo(Pro-Val-Phe-Phe-Pro-Val-Phe-Ser-Leu)，7]、尿嘧啶核苷(uridine，8)、腺嘌呤核苷(adenosine，9)、2-N-(1-甲氧羰基乙基)鸟苷[2-N-(1-Methoxycarbonylethyl)guanosine， 10]。10个化合物均为首次从致命鹅膏中分离得到，其中化合物7和10为新化合物。

In the dehydrogenating process,the anionic A-T fragmentgradually changes its electronic configuration fromπ~* toσ~* state,like the singlebases adenine and thymine.

在脱氢过程中，阴离子A-T的碎片会逐渐改变它的低能电子组态，从π~*态转变到σ~*态，这种变化跟在单个碱基腺嘌呤和胸腺嘧啶的情况相似。

The shorter variant mHemk2 is 1696 bp in length encoding a putative mouse protein of 14.7 kDa, consisting of 138 amino acid residues.In protein data bank annotations, Hemk has previously been classed among methyltransferases methylating N6-adenine or N4-cytidine in DNA because of the presence of S-adenosyl-L-methionine binding motif and of an NPPY motif in the protein.

在蛋白数据库的注释中，由于在Hemk蛋白中存在S-腺苷-L-甲硫氨酸结合基序和一个NPPY基序，因此，在蛋白库的说明中已经将Hemk蛋白归类于能甲基化DNA中N6-腺嘌呤或N4-胞嘧啶的甲基转移酶。

What would he tell Judith and the children?

他将怎样告诉朱迪丝和孩子们呢？

I this is at that time, the opinion with peacockish true girl is full of in a heart.

这就是当时的我，一个心中布满虚荣的女孩子真实的想法。