腺嘌呤

Their detection limits of adenine (S/N=3) of 2.0×10(^-7)mol/L and 1.7×10(^-7)mol/L, respectively.

腺嘌呤和鸟嘌呤的检测限分别为2.0×10(^-7)mol/L和1.7×10(^-7)mol/L。

Based on the previous literatures, the following major innovation works were carried out in this dissertation:(1) The derivatization reaction conditions of ephedrine and pseudoephedrine with sensitive derivation reagent in aqueous system were studied and two new methods were developed for the assay of them by microemulsion electrokinetic chromatography and micellar electrokinetic chromatography with LIF detection. The sensitivity and analysis times were greatly improved compared with the previous reports;(2) A new method of CZE with indirect LIF detection was developed for the simultaneous determination of six coumarin compounds (esculin, esculetin, isofraxidin, genistein, naringin and sophoricoside) with fluorescein as the probe. The proposed method enlarges the application range of LIF detector, and provides new approach for the analysis of certain compounds difficult to derivatize;(3) Based on the above research, an MEKC with indirect LIF detection method for the simultaneous determination of adenine and guanine in DNA extracts from fungus, maize and soybean was established;(4) A new method for the investigation of the complexes formed between human serum albumin and ampicillin sodium under the simulated physiology conditions using laser light scattering technique was developed.

该论文在综述前人工作的基础上，开展了如下未见文献报道的创新性的研究工作：(1)使用灵敏的衍生试剂对麻黄碱和伪麻黄碱在水体系中的衍生反应条件进行了系统研究，建立了微乳电动色谱-激光诱导荧光检测法和胶束电动色谱—激光诱导荧光检测法测定麻黄碱和伪麻黄碱的灵敏分析新方法，与以前的报道相比，灵敏度和分析时间均有很大改善；(2)使用荧光素钠作为背景荧光试剂，建立了同时分析测定六种黄酮类化合物(秦皮甲素、秦皮乙素、异秦皮定、染料木素、柚皮苷和槐角苷)的毛细管区带电泳—间接激光诱导荧光检测新方法，扩大了激光诱导荧光检测的应用范围，对难衍生化合物的分离分析提供了一种新思路和新途径；(3)以荧光素钠作为背景荧光试剂，建立了一种用于同时测定食品提取DNA中的腺嘌呤和鸟嘌呤含量的胶束电动色谱—间接激光诱导荧光检测新方法；(4)用动态和静态激光散射法研究了模拟生理条件下人血清白蛋白与氨比西林钠盐相互作用所形成的复合物，为药物分子与蛋白质的相互作用、药代动力学等的研究提供了一种新思路。

The detection sensitivity of guanine and adenine was improved greatly by activating the glassy carbon electrode electrochemically.

通过对电极的电化学活化，其测定鸟嘌呤和腺嘌呤的灵敏度可以大大提高。

The proposed method can be used to estimate the guanine and adenine contents in DNA with good selectivity in a linear range of 0.25 μg 〓～5. 0μg〓.

该方法可以用来测定DNA中鸟嘌呤和腺嘌呤的含量，具有很好的选择性。

Chapter 5 A new method for the simultaneous determination of adenine and guanine in DNA extracts using MEKC with indirect LIF detection was proposed.

第五章建立了一种用于同时测定DNA中腺嘌呤和鸟嘌呤含量的胶束电动色谱—间接激光诱导荧光检测新方法。

It plays an important role in maintaining the intracellular balance of A and G nucleotides.

该酶所催化的反应对维持细胞内腺嘌呤和鸟嘌呤核苷酸的平衡发挥着重要作用。

In SGC-7901 cells, 5-Fu can interfere with the DNA synthesis and result in the reduction of the DNA content. Besides, it can affect the unsaturation degree of the hydrocarbon chains and alter the external environment of guanine and adenine residues in cancer cells.

这说明5-氟尿嘧啶可以影响DNA的复制合成，降低胃癌细胞中DNA的含量，并且影响癌细胞中脂肪酸链的饱和度以及鸟嘌呤和腺嘌呤的外环境，正常细胞拉曼谱的改变说明5-氟尿嘧啶存在一定的副作用。

Through three-step continual EMS mutation, the adenine autotrophy strain(Ml-3) and the adenine-requiring revertant strain(M2-8) were screened successively, and the resistant strain(M3-20) to ammonium ion variant was further screened.

将MY-1经连续3次EMS诱变，依次选育富含核黄素的腺嘌呤营养缺陷型菌株(M1-3)和腺嘌呤缺陷型的回复突变株(M2-8)。

MethodsOur study was to treat gastric ulcer by invigorating spleen and benefiting vital energy Chinese drugs through clinical observation and experimental research, and compared with mucosa protectant -gastropine and H2 receptor antagonist -famotidine about the mucosa mitochondrion 8-oxygen guanine DNA glycosylase, thymine glycol DNA glycosylase,3-methyl adenine DNA glycosylase, the effect of gastralgia, splenic asthenia and quality of ulcer recovery.

比较健中愈疡片、黏膜保护剂胃舒平以及H2受体拮抗剂法莫替丁对胃黏膜线粒体 8－氧鸟嘌呤DNA糖基化酶、胸腺嘧啶乙二醇DNA糖基化酶、3－甲基腺嘌呤DNA糖基化酶的影响，并进行健脾益气方药与黏膜保护剂胃舒平以及H2受体拮抗剂法莫替丁对胃溃疡愈合质量的比较和评价。

Thermally denatured DNA was preconcentrated at pretreated GCE by its adsorption in open circuit for 5min or at the potential of+0.3V for 90s, and produced two well-defined oxidation peaks of guanine and adenine residues at+0.80V and+1. 11V in pH 5. 0 phospoate buffer, respectively, but the native DNA did not produce any peaks at the same conditions.

在pH 5.0的磷酸盐缓冲液中，于+0.3V富集90秒或在开路条件下吸附富集5分钟，热变性DNA可以吸附富集在预处理玻碳电极表面，阳极扫描时，鸟嘌呤和腺嘌呤残基产生两个很好的氧化峰，峰电位分别为+0.80V和+1.11V，而在同样条件下，天然DNA却几乎不出现峰。

What would he tell Judith and the children?

他将怎样告诉朱迪丝和孩子们呢？

I this is at that time, the opinion with peacockish true girl is full of in a heart.

这就是当时的我，一个心中布满虚荣的女孩子真实的想法。