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Methods In this study,a plasmid vector,rAd5,and rAAV2 were used to transfect rat cochlear marginal cells of stria vascularis by injection into the perilymph through the round window membrane,and the transfection efficiency, target tissue accessibility, cell/ tissue toxicity, time course of expression, and effect on hearing were evaluated in vivo and in vitro.

结果三种载体对原代培养的大鼠耳边缘细胞的转染研究发现,腺病毒的转染效率最高,而腺相关病毒转染对细胞活性影响最小。rAAV2和rAd5对大鼠耳蜗组织的感染实验发现,腺病毒和腺相关病毒携带的增强型绿色荧光蛋白可在多种耳蜗组织中及转染对侧耳蜗组织表达,且并不引起明显的耳蜗组织细胞凋亡。rAAV2携带的EGFP基因在第90天时仍可检测到大量表达。rAd5携带的EGFP基因在第30天时表达明显减弱。

The results indicate that all of the peltate glandular hairs and head glandular hairs are from an origin—protoderm on the leaf primordium and young leaf, which outgrows on leaf surface and increase radial broad and form the initial cells of the glandular hairs.

研究结果表明,唇形科的腺毛不论是盾状腺毛类还是头状腺毛类,在发育上都是单起源的,即所有的腺毛均起源于叶原基或幼叶上的原表皮细胞。该细胞在叶表面向外突出生长,径向加宽,形成腺毛原始细胞。

In 1 series of primary salivary gland tumors,local recurrences preceded distant metastases in all cases of ACA.

在一组对涎腺原发肿瘤的研究中,所有的腺泡细胞腺癌在远处转移之前均见局部复发。

Immunohistochemistryand image analysis was used to semiquantitatively detect the expression of HO-1 and MRP2 protein.

结果:原发胆囊癌组织HO-1和MRP2表达的总阳性率分别为73.1%、77.6%。其中腺癌的阳性率为76.7%和80%。

According to the shape of head, the glandular hairs were divided into capitate and pehate glandular hairs.

根据腺毛头部形状可把腺毛分为盾状腺毛和头状腺毛,二者均发生得很早,它们的原始细胞都来源于原表皮,一般在茎尖第1和第2片叶原基的原表皮细胞以及茎的1~2节间原表皮细胞中开始发生。

In the normal uterus, Cytokeratins immunolabelling were detected in glandular cell, luminal epithelial cell, Vimentin immunolabelling were detected in stromal cell and endoblastic cell; CK7 immunolabelling were not detected in any tissue of the yak utenus.

研究结果显示:未妊娠时,泛角蛋白在子宫内膜腺上皮细胞、腔上皮细胞内表达,波形蛋白在子宫内膜基质细胞内表达,平滑肌肌动蛋白在子宫平滑肌和血管平滑肌内表达,牦牛子宫任何部位均不表达角蛋白7;妊娠30天左右时,泛角蛋白在子宫内膜腺上皮细胞、子宫内膜腔上皮细胞、滋养层细胞、内胚层细胞和尿囊细胞内表达,波形蛋白在子宫内膜基质细胞和内胚层细胞内表达,平滑肌肌动蛋白在子宫平滑肌和血管平滑肌内表达,角蛋白7在尿囊细胞内表达,偶尔在腔上皮细胞的细胞核边缘表达;消化法进行原代培养时,组织经胶原酶消化并通过100目和400目筛网组合可以有效地分离原代子宫内膜基质细胞和子宫内膜腺上皮细胞;分离得到的子宫内膜基质细胞活率达90%以上,并可在体外传代7次以上;分离得到的子宫内膜腺上皮细胞活率可达85%以上,并可在体外传代5次以上;RPMI1640培养基最适合子宫内膜基质细胞和子宫内膜腺上皮细胞的生长,维持子宫内膜基质细胞正常生长的FBS添加量为20%,维持子宫内膜腺上皮细胞正常生长的胎牛血清添加量为30%。

Results The concentration of Chlorogenic acid was 0.05mg/ml, 0.1 mg/ml,0.4mg/ml,0.8mg/ml respectively,it can respectively inhibit respiratory syncytia virus,Coxsackie B 3 virus,adeno associated 7 virus,adeno associated 3 virus and Coxsackie B 5 vi...

结果 氯原酸浓度分别为 0 。0 5mg/ml、0 。1mg/ml、0 。4mg/ml、0 。8mg/ml时,可分别体外抑制合胞病毒、柯萨奇B3、腺病毒 7型、腺病毒 3型和柯萨奇B5型。结论氯原酸对常见呼吸道病毒有较强的抑制作用

Groups of 16,20mg significantly increased the level of hormones of serum, such as insulin,growth hormone, triiodothyronine(T3),thyronine(T4except the group of 16mg),insulin-like growth factor-1 (IGF-1),but there was no significant effect on the level of serum glucose.3 , All dosage can significantly increase the crude protein(except the group of 20mg) and significantly decrease crude fat (p.05);and they had significanteffect on some parameters of body stature, such as length of body slope, length of chest bone, girth of chest, pelvic breadth, metatarsus length, metatarsus girth.

不同剂量的G98均能提高肉鸡的激素水平,其中16、20mg/Kg的两个剂量组能显著性提高血清中胰岛素、生长激素、类胰岛素生长因子-1(IGF-1)、三碘甲腺原氨酸(T_3)、甲状腺素(T_4,16mg/Kg剂量组除外)的水平;但对血糖水平没有显著性影响。

Expression of β-catenin in metastatic carcinomas was significantly lower than that in primary colon carcinomas (p.01). expression of e-cadherin in metastatic carcinoma was significantly lower than that in primary colon carcinoma(p.01),especially in tubiform adenocarcinoma and well and moderately differentiated carcinoma.

结果] 原发灶及其淋巴结转移灶中β-结肠术后多久的正常表达率分别为23.46%和4.94%,原发灶中术后多久高于转移灶(p.01),尤其是高中分化病例,转移灶中异位表达率显著高于原发灶(45.68%比28.40%,p.01),尤其是管状腺癌;原发灶中β-cat表达强度显著高于转移灶(p.01)。e-cad的表达强度在原发灶显著高于转移灶(p.01),尤其是管状癌症治疗和高中分化病例。

The first functional lymphoid organ was head kidney, different from that of other marine teleosts: thymus was the first lymphoid organ normally.

孵化后即出现肾脏原基,8日龄和12日龄分别出现脾脏和胸腺原基;而免疫器官淋巴化的先后顺序较为特殊,首先淋巴化的器官是头肾,其次为胸腺和脾脏,不同于大多数海水鱼类。

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