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OBJECTIVE: Three sesquiterpene lactones 1-O-acetylbritannilactone, isoalantolactone and britannilactone were isolated from the roots of Inula helenium and the flowers of Inula japonica. The antitumor activities of the three sesquiterpenoids in the cell lines of HeLa, HEC-1, SHIN3, HOC-21 and HAC-2 were measured, and the relationship between structure and activity and the possible mechanisms were explored.

目的: 观察菊科植物中分离出的3种倍半萜化合物异土木香内酯、1-氧-乙酰大花旋覆花内酯和大花旋覆花内酯对体外培养的人子宫颈癌HeLa细胞、人子宫内膜癌HEC-1细胞、人卵巢透明细胞癌SHIN3及HOC-21细胞和人卵巢囊腺癌HAC-2细胞增殖的影响,研究这3种倍半萜化合物的体外抑制肿瘤细胞增殖作用及其结构与活性之间的构效关系,进而探讨3种倍半萜化合物的体外抑制肿瘤细胞增殖的可能机制。

Pituitary hypophysis is themostcomplex body oftheglands, hormones produced by the body andnotonly bone and thesofttissue growth, and can affect theotherendocrine glandsactivities.

脑垂体hypophysis是身体内最复杂的内分泌腺,所产生的激素不但与身体骨骼和软组织的生长有关,且可影响其它内分泌腺的活动。

Pituitary hypophysis isthemostcomplex body of theglands, hormones produced by the bodyandnotonly bone and the softtissue growth, and can affecttheotherendocrine glandsactivities.

脑垂体hypophysis是身体内最复杂的内分泌腺,所产生的激素不但与身体骨骼和软组织的生长有关,且可影响其它内分泌腺的活动。

Wenxin capsule can promote endolethial cells releasing CO and synthesing 〓, enhance activity of HO, induce angiectasis through activating guanylic cyclase and adenglate cyclate of intracellar of vessel smooth muscle cell to enhance content of intracellar cGMP and AMP. 3. Wenxin capsule can inhibit releasing of platelet activiting factor 〓 which inhibit platelet aggregation and have antithrombosis function. 4. Wenxin capsule can promote activity of t-PA, reduce activity of PAI-1, strengthen fibrinolytic function, therefore inhibit fibrin disposition. 5. Wenxin capsule can inhibit synthesis of smooth muscle cellular DNA. Its function don't kill it directly but prevent transformation from period Go to peried S. 6. Wenxin capsule is an effective prescription of anti-coronary atherosclerosis atherosis. The results of image pattern analysis tastifies that area of lipid plaque in treating group reduce 54% and 58% in contrast with that in control group. The results of light, electro- microscopy show that structure of endolethial cell and smooth muscle cell in treating group is more integral than that in control group.

其作用机制之一是由于该药具有良好的调脂降脂作用,其不仅能明显降低血清LDL-c及升高HDL-c含量,且能有效阻止TC沉积于血管壁,从而保护内皮细胞结构完整;此外,温心胶囊尚具有明显的抗氧化作用,该药不仅能明显降低血浆LPO含量,且能提高SOD活性,同时其对血红素在细胞内代谢的调节作用也参与了抗氧化过程。2 温心胶囊能明显促进内皮细胞释放CO及合成〓,提高HO活性,通过激活血管平滑肌细胞内鸟苷酸环化酶及腺苷酸环化酶引起细胞内cGMP、AMP浓度增加而引起血管舒张。3 温心胶囊能明显抑制血小板激活因子〓的释放,从而抑制血小板聚集,抗血栓形成。4 温心胶囊能明显提高t-PA活性,降低PAI-1活性,增强纤溶功能,从而抑制纤维蛋白沉积。5 温心胶囊能明显抑制平滑肌细胞DNA合成,其抑制作用不是直接杀伤抑制,而是阻止细胞由Go期向S期转化。6 温心胶囊是抗冠状动脉粥样硬化的有效制剂,图象分析结果证实,该药治疗组脂斑面积比对照组缩小了54%和58%,光电镜结果显示内皮细胞及平滑肌细胞结构较为完整。

The location and structures of sex-pheromone-producing gland in female H.insularis were studied by EAG,GC,SEM,and TEM.These studies showed that thegland situate in the intersegmental membrane between the eighth and ninthabdominal segments,and is an eversible abdominal fold;Many plump cones disturbon the surface of the gland.The glandular cells of 2-day old virgin female H.insularis are arranged in one layer,among which the central cells are columnarepithelial cells and flat on two sides.The nucleus is irregular elliptical.There isevident conjugation between cells and the involution is more in the basal membraneof cell.Microvilli are distributed on the cytoplasmic membrane and linked withendocuticle on which there are many layers of chitin,and the outer cuticule is staineddeeper.The cell contains bubbles,mitochondria,glycogen deposits,roughendoplasmic reticulum and smooth endoplasmic reticulum.

结合触角电位、毛细管气相色谱、扫描电镜、透视电镜等技术对小线角木蠹蛾雌蛾腹尖末端不同组织部位提取物的测定分析以及腺体位置和形态结构的观察发现:小线角木蠹蛾性信息素分泌腺位于腹部末端8~9节之间,是一个由节间膜特化而成的上皮结构,为一可外翻的腹褶,腺体表面分布着饱满的锥形体,羽化后2天未交尾的雌蛾腺体细胞呈单层排列,腹面中央由密集的柱形上皮细胞组成,细胞排列向两侧延伸至背部,其形状由柱形逐渐变为扁平形,细胞核为椭圆形,细胞与细胞间有明显的胞连接,细胞基底膜基褶较多,质膜上分布着微绒毛,并与内表皮连接,内表皮上有多层几丁质,外角质层染色较深,细胞质中含有空泡、线粒体、脂质粒、粗面内质网和光面内质网。

Hypothalamic-pituitary functions in eight children with intrasellar and suprasellar tumors (five craniopharyngioma, one epidermoid cyst, one germinoma, one pituitary adenoma) were studied before and after surgical treatment. Three children who received endocrine function assessment before operation showed at least one hormone deficiency.

本文收集8例患有蝶鞍内或蝶鞍上方肿瘤的病童,分析手术前或手术后的下视丘一脑垂腺机能,所有於手术前接受下视丘一脑垂腺机能评估的病童,皆至少有一个荷尔蒙分泌异常,其中以生长激素及促性腺激素的异常最常见。

Results Cuticular papillates,glandular hairs,peltate scales,and non-glandular hairs presented on the leaf epidermis of C.paliurus.Anomocytic stomata were confined to the lower epidermis.Large cluster crystals existed in the uppermost layer of palisade cells.Midrib included one closed vascular system and one additional vascular strand.Secondary veins are semicraspedodromous and form marginal loops,tertiary veins are percurrent,quaternary veins form orthogonal or polygonal areoles filled up by branched and freely ended veinlets.

结果青钱柳叶表皮有许多小乳突,腺毛,盾状鳞片和非腺毛表皮毛;气孔器不规则型,仅分布于下表面;靠近表皮的叶肉组织中有规律地分布含簇晶的细胞;主脉中维管组织环状,封闭,具有一个副维管束;二级脉在叶缘内分支,相互连接形成脉环,三级脉及顶型,脉间区内充满分支的自由结束的小细脉。

Moreover, overpression of Wnt10b in the organ culture of vibrissae HF has been manipulated by infected the recombinant adenovirus expressed Wnt10b protein(Adwnt10b).

Ki67在毛母质中的表达变化规律以及在毛母质中的表达部位与Wnt10b具有一致性。2、我们对Wnt10b重组腺病毒进行了逐级扩增并应用氯化铯超高速密度梯度离心法纯化,病毒最终滴度达到109 PFU/ml;3、首次发现加入Wnt10b重组腺病毒可明显促进体外器官培养的触须毛囊毛干和内根鞘生长,这与本课题组在在体动物实验中发现的现象一致。

The remaining eighteen pigs, served as three viral administered groups, underwent coronary artery ligation and received intramyocardial viral injection. rAAV- GFP group (n=6), rAAV-CD151 group (n=6) and rAAV-antiCD151 group (n=6) animals respectively received direct intra- myocardial injection of rAAV-GFP, rAAV-CD151 and rAAV-antiCD151 1×10~(12 viron particles per pig, at 10 sites correspondingly.

采用三质粒共转染法包装CD151、antiCD151和GFP重组腺相关病毒(recombinant adeno-associated virus,rAAV)。2.1月龄小型猪22头,随机分为4组,分别为正常对照组4头(不予冠脉结扎和注射病毒,正常喂养),rAAV-GFP组6头结扎冠状动脉左前降支,心肌内分10点注射1×10~(12viron particles的rAAV-GFP病毒,rAAV-CD151组6头结扎冠状动脉左前降支,心肌内分10点注射1×10~(12viron particles的rAAV-CD151病毒,rAAV-antiCD151组6头结扎冠状动脉左前降支,心肌内分10点注射1×10~(12vironparticles的rAAV-antiCD151病毒。

Ultrastructurally, the nuclear membranes of lymphocyte and reticular cell were broken; the rough-surfaced endoplasmic of lymphocyte, reticular cell and plasma cell were swelling in immune organs; the crista of mitochondrion was broken in plasma cell of spleen.

胸腺、法氏囊中淋巴细胞和网状细胞的核膜局部断裂,粗面内质网肿胀;脾脏中浆细胞线粒体嵴断裂,粗面内质网肿胀。

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