腺

Because different cell lines may show various levels of susceptibility to adenovirus infection and virus production, non-selectively replicating wtAd5 was used to be a comparison with the tested conditionally replicating adenovirus CNHK500.ONYX-015, the first replication-competent adenovirus entered into clinical trials was also used to be a comparison. We used virus proliferation assay, cell viability assay and western blot to evaluate the proliferation and cytolysis selectivity of CNHK500. Results demonstrated that the CNHK500 virus proliferation multiples in breast cancer cell lines after 48 hours is similar to that of wtAd5, ONYX-015 virus proliferation ability is less than that of CNHK500 in cancer cells.

然后，通过与ONYX-015（E1B 55 KD a蛋白缺失的2型和5型嵌和型腺病毒，美国ONYX生化制药公司研制）、wtAd5进行对比，利用病毒增殖实验和细胞生长抑制实验，验证了CNHK500选择性增殖能力和肿瘤特异性杀伤作用；利用Western Blot检测CNHK500腺病毒E1A和E1B在乳腺癌细胞和正常成纤维细胞中的表达差异，揭示了腺病毒肿瘤靶向性的机制；利用携带绿色荧光蛋白的CNHK500-EGFP感染乳腺癌细胞株和正常成纤维细胞株，直观地观察其感染乳腺癌细胞的能力和在乳腺癌细胞中的增殖过程。

All form ATP from adenosine monophosphate or adenosine diphosphate and inorganic phosphate.

三磷酸腺都是由腺一磷酸或腺二磷酸和无机磷酸形成。

The strong positive expression located in the gland alveolus and duct of serous gland, as well as myoepithelium of mucus glands. In some cases, blood vessel endothelium and fibrous connective tissue also show weakly positive expression of NF-κB.

NF-κB的表达主要见于浆液腺腺泡上皮和导管上皮，呈强阳性表达；黏液腺肌上皮样上皮呈强表达；间质成分中血管内皮呈弱阳性表达、纤维结缔组织呈弱阳性表达。

In 1988 a lacrimal gland tumor was incompletely removed from the left orbit of a 36-year-old woman and diagnosed as "solid basaloid adenoid cystic carcinoma". Soon after, an exenteration of the orbit was performed. In 1998, upon review of the initial histopathology, the diagnosis of solid adenoid cystic carcinoma was changed to basal cell adenocarcinoma..

方法；1988年一个36岁的妇女的泪腺肿瘤从左眼眶不完全摘除，诊断为"实质性基底细胞囊腺癌"，不久进行了眼眶内容去除术。1998年对原始的组织病理学进行回顾，实质性基底细胞囊腺癌被更正为基底细胞腺癌。

I will apply Immumohistochemical method to detect proliferating cell nuclear antigen expression of lung adenocarcinoma histiocyte inpatients, and I will study apoptosis of lung adenocarcinoma cells by TUNEL method; calculate PCNALI and ALI; compare and analysis PCNALI and ALI of different patients with different clinic features simultaneously .

例肺腺癌组织中增殖细胞核抗原的表达，同时通过TUNEL法检测肺腺癌细胞凋亡，计算PCNA增殖指数和细胞凋亡指数，对不同临床特点肺腺癌患者的PCNALI、ALI进行比较分析。

Apoptosis of lymphocytes in bone marrow, spleen, thymus and bursa was detected by light microscope, electron microscope and terminal- deoxynucleotidyl transferase UIP nick end labeling. The results showed ALV-J could induce incoordinate apoptosis of lymphocytes in thymus, bursa and spleen, which was mainly found in early time. And the apoptosis was much more obvious in thymus than in other immune organs.

以电镜观察和HE染色、原位末端标记染色光镜观察对ALV-J感染和混合感染鸡的骨髓、脾脏、胸腺、法氏囊等免疫器官中淋巴细胞的凋亡进行了形态学研究，首次发现ALV-J可诱导胸腺、法氏囊和脾脏中不同程度的淋巴细胞凋亡，凋亡主要见于早期，ALV-J与REV混合感染时淋巴细胞凋亡尤为显著，以胸腺中最为明显。

The result indicate that the DPV DNA can be firstly detected 2 hours after inoculability from brain,liver,spleen,bursa of fabricius,thymus.

结果表明，接种2小时后即能够从脑、肝、脾、法氏囊、胸腺中检出DPV DNA.12小时，即可从心脏、肝脏、脾脏、肺脏、肾脏、十二指肠、直肠、法氏囊、胸腺、胰腺、脑组织、胸肌、食道、腺胃、血液、舌头、口腔分泌物、皮肤、骨髓和粪便等20种组织器官中检测到DPV的DNA。检出时间最早和检出率最高的组织器官为肝脏和脑组织。

The high scores of AgNOR and the positive of C-erbB-2 indicates a high degree of malignance of patients with endometrial carcinoma.

AgNOR值增高提示患者可能患有子宫内膜腺癌且提示子宫内膜腺癌恶性程度高，如合并C-erbB-2蛋白的阳性表达率增高可进一步提示患者患有子宫内膜腺癌。

Such cancer is characterized by its moistness , the vagueboundary with its surrounding tissues , the relapse after operation , the intrusion intovessels and the metastasis to remote parts through blood circulation .

涎腺腺样囊性癌是较为常见的涎腺恶性肿瘤之一，具有浸润性强，与周围组织界限不清，术后易复发；易侵入血管，沿血循环发生远处转移等特点。

Using SMARTTM cDNA library construction technology from Clontech biocompany,we have constructed 4 Matchmaker cDNA libraries for posterior silk gland of 4th moulting larvae,posterior silk gland,middle silk gland and fat body of larvae at 3rd day of 5th instar, respectively.

家蚕的丝腺是一个特异化的器官，主要用于合成蚕丝蛋白，包括在家蚕的后部丝腺表达的丝素重链蛋白、丝素轻链蛋白、P25蛋白(fibrohexamerin，Fhx)以及在家蚕中部丝腺表达的丝胶蛋白。

The absorption and distribution of chromium were studied in ryeusing nutrient culture technique and pot experiment.

采用不同浓度K2CrO4(0，0.4，0.8和1.2 mmol/L)的Hoagland营养液处理黑麦幼苗，测定铬在黑麦体内的亚细胞分布、铬化学形态及不同部位的积累。

By analyzing theory foundation of mathematical morphology in the digital image processing, researching morphology arithmetic of the binary Image, discussing two basic forms for the least structure element: dilation and erosion.

通过分析数学形态学在图像中的理论基础，研究二值图像的形态分析算法，探讨最小结构元素的两种基本形态：膨胀和腐蚀；分析了数学形态学复杂算法的基本原理，把数学形态学的部分并行处理理念引入到家实际应用中。