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We compared the difference of gene expression between thymus in one-and ten-month old Balb/c micee using DDRT-PCR. It was found that there would be a significant difference of gene expressions in murine thymuses.

我们采用差异显示反转录PCR技术对1月和10月龄Balb/c小鼠胸mRNA的差异表达情况进行了分析,共获得月龄差异表达的cDNA片段108条(其中90条源于1月龄小鼠胸中,而有18条源于10月龄小鼠胸中)。

The thymuses, spleens, sera and anticoagulant blood were collected after the rats were killed, to carry out these works:(1) Zinc concentrations were analyzed by atomic absorption spectrophotometry;(2) Body weight and the weight of thymuses and spleens were measured, the organ index were calculated;(3) Changes in pathology of thymus and spleen were observed;(4) Using TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling) method, the apoptosis of thmocytes and spleen lymphocytes was checked;(5) The expression of bcl-2、bax mRNA in thymus and spleen were detected by RT-PCR (reverse transcription polymerase chain reaction);(6) The expression of p56〓, a signal transduction protein in thymus and spleen were detected by immunohistochemistry;(7) Two-color cytofluorometric analysis was used to assess the expression of CD4, CD8, CD45RA and CD45RC in peripheral blood lymphocytes.

动物处死后留组织、抗凝血及血清,进行以下检测:(1)原子吸收法测血清锌浓度;(2)测胸、脾脏脏器重量并与大鼠体重比较,计算脏器指数;(3)HE染色对胸、脾脏进行病理观察;(4)原位末端标记法测胸、脾脏中淋巴细胞的自发凋亡,并通过图像分析进行比较;(5)逆转录聚合酶链式反应检测凋亡调控基因bcl-2、bax mRNA在免疫器官中的表达;(6)免疫组化方法检测信号转导蛋白p56〓在免疫器官中的表达;(7)流式细胞术分析外周血淋巴细胞中CD4+、CD8+、CD45RA+及CD45RC+CD4+、CD45RC-CD4+细胞的数量和比例。

The thymuses of ZD rats appeared atrophy, with thin cortex, unclear boundary of thymus cortex and mudulla, and decreased thymocytes.

3显微镜下观察,ZD组大鼠胸明显萎缩,皮质变薄、疏松,皮、髓质分界模糊,胸细胞明显减少,胸小体的数量也明显减少。补锌后,ZS组胸的病理结构有明显恢复,与ZA〓组无明显差异。

A hormone secreted by the posterior lobe of the pituitary gland that constricts blood vessels, raises blood pressure, and reduces excretion of urine.

性垂体前叶脑下垂体性前叶,可分泌包括促肾上皮质激素、乳汁刺激素以及促长激素的多种激素

RhoGDI (RhoGDP-dissociation inhibitor 1) and Cyclophilin A (Peptidyl-Prolyl Cis-Trans Isomerase A) were reported to differentially express in two studies, but their results were inconsistent.

其中mimecan在正常结直肠组织中强表达,却在瘤和癌组织中不表达;TXNDC5(Thioredoxin domain-containing protein 5)在正常,瘤和癌组织中都表达,瘤和癌组织的表达量显著高于正常(P<0.05);RhoGDI(Rho GDP-dissociation inhibitor 1)和Cyclophilin A(Peptidyl-Prolyl Cis-Trans Isomerase A)在两份研究中报道差异表达,却都是一个报道在结直肠癌中高表达,一个报道低表达。

RESULTS The results indicated that PCF could significantly increase the viability of thymocytes and enhance the activities of SOD, GSH-Px, CAT in cells. PCF decreased the amounts of ROS and the rate of apoptosis in cells. PCF inhibited the morpholog alteration of the thymocytes irradiated by UVB and maintained the ultrastructure of cells in normal. PCF attenuated UVB caused DNA fragmentation in thymocytes. While combined ERKs inhibitor yielded typical DNA fragmentation, co-treatment of thymocytes with PCF and JNKs and p38 kinase inhibitors completely blocked UVB-induced DNA fragmentation.

结果 PCF能显著提高UVB辐射后小鼠胸淋巴细胞的活性;提高胞浆中SOD、GSH-px、CAT的活性,降低ROS含量及细胞凋亡率;抑制UVB辐射所致的胸淋巴细胞凋亡的形态学改变,维持细胞的正常超微结构;抑制UVB所致的胸淋巴细胞DNA片段的出现;预先应用ERK抑制剂使DNA片段增加,给予JNK和P38抑制剂后协同PCF使DNA片段带减少。

The numbers of ANAE(superscript +) T cells in the spleen and head kidney were more than those of thymus, therefore, it is suggested that the matured T cells are emigrated thymus to spleen and head kidney.

实验结果进一步证明了胸中的T细胞多为不成熟的T细胞,此外,还含有大量的IgM细胞;而脾脏、头肾内的ANAE T细胞多于胸,推测胸成熟的T淋巴细胞迁移到头肾和脾脏。

The border of hyperplastic nodal which had no pathological nuclear mitoses, invasion and necrosis was clear. The staining of 8 antidodies had not significant value to the differential diagnosis between basal cell adenocarcinoma and adenoma of prostate.

而基底细胞瘤内增生的基底细胞异型性不明显,无病理性核分裂象,增生结节周界清,无浸润性边缘和坏死。8种抗体的免疫酶标记在前列基底细胞癌和瘤的鉴别诊断上无显著性意义。

Results Basal cell adenocarcinoma of prostate was composed of poorly differentiated solid carcinoma nests with many cellular nuclear mitoses arranged like basal cell carcinoma. Necrosis was in the middle of the carcinoma nest. Local differentiation to squamous cell, transitional cell and gland and structure like adenoid cystic carcinoma were found.

结果:前列基底细胞癌为分化差的实体癌呈基底细胞癌样排列,核分裂象多,癌巢中央伴坏死,可见局灶性鳞状细胞、移行细胞或管分化,也可出现样囊性癌样结构。

Methods The genomic DNA was extracted from murine thymocytes and splenocytes for PCR amplification of the target fragments.

推测其中的初始T细胞的数目,评价胸功能,从而为胸功能研究及胸研究模型的建立提供研究依据和方法。

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