腹腔妊娠

Methods: Separate and abstract the effective components of Huangqi (Omni-Saponin、total-flavone) and Danshen (Danshen-ketone、 Danshen-Quinone). Applying L-Arginine methyl ester to pregnant rats by I. P to make Nitrogen Oxide Synthesis blocking models (uterus-placenta-fetus lacking blood and oxygen model or pregnant hypertension syndrome model). On day 12, 18 of pregnancy paunched the rats and took out the fetus and placenta/uterus. Measured the basal BP during pregnant period and BP、protein、basal activity each day. Observed placenta/deciduas ultramicroscopic structure by electron microscope and took count of languishment cells. Measured the expressive intensity of bcl-2、Bax of placental nutrient cells by SABC method.

一氧化氮合成酶阻滞剂L-精氨酸甲酯妊娠大鼠腹腔给药，制作一氧化氮合成阻滞模型（子宫-胎盘-胎儿缺血缺氧或妊娠高血压模型），妊娠第12、18天剖腹取胎、胎盘／子宫，妊娠期测基础血压，每日测血压、蛋白质量、基本生理活动；电镜观察胎盘／蜕膜超微形态结构，并进行细胞凋亡计数；免疫组化检测胎盘滋养细胞bcl-2、Bax表达强度；原位杂交法检测胎盘滋养细胞、血管内皮细胞VEGF、eNOS、iNOS、MCP-1、MMP9（基质金属蛋白酶9）mRNA表达。

In order to investigate the effect of IFN-γon the expression of TNF-αin the hypothala- mus-pituitary-ovary axisof RU486-induced aborted SD rats, RU486 was used to establish the abortion model, then the aborted rats were administrated with different doses of IFN-γintraperitoneal injection, the method of immunohistochemical ultrasensitive SP was us- ed to study the variation of positive TNF-αcells in hypothalamus, pituitary, ovary and uterus in normal pregnant group A, aborted model group B and three different doses of IFN-γgroup C,D,E. The main goal of this research was to study whether the low dose of IFN-γcould have a protective function to pregnancy and the mechanism of its function so as to provide morph- ologic basis for the scientific using of IFN-γin the process of animal abortion control.

为探讨IFN-γ对流产大鼠下丘脑-垂体-卵巢轴(hypothalamus-pituitary- ovary-axis，HPOA )TNF-α表达的影响，本试验用米非司酮(RU486)建立SD大鼠流产模型，通过腹腔注射不同剂量IFN-γ，采用超敏感免疫组织化学SP法(链霉素抗生物素—过氧化物酶法)，观察了孕13天(d13)SD大鼠正常妊娠A组、流产模型B组、不同剂量IFN-γC、D、E组下丘脑、垂体、卵巢、子宫TNF-α的表达变化，探讨低剂量IFN-γ对妊娠是否有保护作用及其作用机制，期望能为在妊娠过程中如何科学应用IFN-γ防止动物流产提供形态学依据。

Results 10 cases of PEP occurred after laparoscopic surgery,and 5 happened at laparotomy. 3 cases after salpingectomy,and 12 after after conservative operation of tubal pregnancy.

结果 持续性异位妊娠发生于腹腔镜手术后有10人，发生于开腹手术5人，持续性异位妊娠发生于输卵管切除术后3人，发生于保留输卵管后的有12人。

Objective To explore the value of laparoscopic operation in the treatment of gynecologic diseases.Methods Retrospective analysis of 170 cases of laparoscopic surgery in ynecology.53 cases in the salpingocyesis group,49 cases salpingotomy,4 cases salpingectomy;48 cases in the ovarian cyst group,35 cases enucleation and repair of ovary,8 cases ovary sutures atches,3 adnexnectomy and 2 perforation of ovary;52 cases in the uterine problems group,10 cases LM5 cases CISH,11 cases LSH（laparoscopic supracervical ysterectomy）,14 cases LTH（laparoscopic total hysterecotomy）,12 cases LAVH laparoscopically assisted vaginal hysterectomy）;17 cases in the infertility group were studied in terms of lysis of pelvic adhesions and salpingostomy.

目的 探讨妇科腹腔镜手术的临床应用价值方法回顾性分析170例妇科腹腔镜手术病例，其中异位妊娠组53例，行输卵管切开取胚49例，输卵管切除术4例；卵巢疾患组48例，囊肿剥除术及卵巢成形术35例，卵巢缝合修补8例，患侧附件切除3例，囊卵巢多点打孔2例；子宫疾病组52例，行肌瘤剥除10例，改良CISH术5例，子宫次全切除术11例，子宫全切术14例，腹腔镜辅助阴式子宫切除12例；不孕症组17例，行盆腔粘连分解输卵管伞造口术。

objective:to explore the value of laparoscopy in diagnosis and treatment of female infertility.methods:the clinical data of 139 cases of female patients with infertility were analyzed retrospectively.results:among 139 cases,55 cases with pelvic adhesion,34 cases with salpingitis,20 cases with endometriosis,and 14 cases with polycystic ovary.the pregnancy rates were 47.27%,41.18%,55.56% and 46.15% respectively after laparoscopic surgery.pelvic adhesion salpingitis and endometriosis were the major cause of female infertility.conclusions:laparoscopic technique can find out the reason of infertility more earlier and more directly and provide timely therapy.so it has excellent clinical effect.

目的：探讨腹腔镜诊治女性不孕症的应用价值。方法：回顾分析我院2004年12月至2007年12月应用腹腔镜诊治女性不孕症139例患者的临床资料。结果：139例中盆腔粘连55例，输卵管炎34例，子宫内膜异位症20例，多囊卵巢综合征14例，术后妊娠率分别为47.27%，41.18%，55.56%，46.15%。盆腔粘连、输卵管炎、子宫内膜异位症是引起女性不孕的主要盆腔病因。结论：应用腹腔镜技术能早期、直观、准确的明确不孕症输卵管及盆、腹腔病因并可同时予以针对性治疗，在直视下通液，动态观察输卵管通畅度和形态，大大降低了常规通液术的假阳性、假阴性率。

Results All cases became amenorrheic during GnRH-α therapy. The enlarged uteri all decreased to normal or near normal size. Menstruation returned in 80～90 days after cessation of treatment. Three cases conceived within four menstrual periods. One of them resulted in the birth of a healthy 3 150 g male at 38 weeks gestation by cesarean section. The second pregnancy resulting after adenomyomectomy was terminated by emergent cesarean section at 30 weeks gestation because of threatened rupture of uterus. The third is now normal at 28 weeks pregnancy .The fourth has had 2 menstrual periods and is still being followed up.

结果 GnRH-α用药期间4例均闭经，血雌二醇于用药的第3周降到绝经期水平；用药6次后增大的子宫缩小至正常或接近正常。1例子宫后壁局限性腺肌瘤未行手术切除者，用药后腺肌瘤缩小73.7%。3例于腹腔镜术后、停药转经的4个月内妊娠；另1例停药转经2个月，现在随访中。3例妊娠转归：1例足月剖宫产分娩；1例手术切除肌壁间腺肌瘤者，孕30周时因先兆子宫破裂急诊行剖宫产术；1例目前孕22周。

Their fecundity outcome were followed-up after cessation of GnRH-α treatment. Results All cases became amenorrheic during GnRH-α therapy. The enlarged uteri all decreased to normal or near normal size. Menstruation returned in 80～90 days after cessation of treatment. Three cases conceived within four menstrual periods. One of them resulted in the birth of a healthy 3 150 g male at 38 weeks gestation by cesarean section. The second pregnancy resulting after adenomyomectomy was terminated by emergent cesarean section at 30 weeks gestation because of threatened rupture of uterus.

结果 GnRH-α用药期间4例均闭经，血雌二醇于用药的第3周降到绝经期水平；用药6次后增大的子宫缩小至正常或接近正常。1例子宫后壁局限性腺肌瘤未行手术切除者，用药后腺肌瘤缩小73.7%。3例于腹腔镜术后、停药转经的4个月内妊娠；另1例停药转经2个月，现在随访中。3例妊娠转归：1例足月剖宫产分娩；1例手术切除肌壁间腺肌瘤者，孕30周时因先兆子宫破裂急诊行剖宫产术；1例目前孕22周。

Methods: The clinical data of 41 patints with rare ectopic pregnancy including interstitial pregnancy.23 cornual uterogestation,9 ovarian pregnancy,4 abdominal,2 pregnancy were retrospectively analyzed,deformity uterogestation,1 intramural pregnancy,1 and nub of fallopian tube pregnancy,were retrospectively analyzed.

回顾 分析 2003年7月至2005年10月经腹腔镜手术治疗的特殊部位异位妊娠41例的临床资料，包括输卵管间质部妊娠23例，宫角妊娠9例，卵巢妊娠4例，腹腔妊娠2例，残角子宫妊娠1例，子宫肌壁间妊娠1例，输卵管残端妊娠1例。

Eccyesis the good peritoneoscope surgery treats, now eccyesis the surgery treats the best method.

异位妊娠行腹腔镜手术治疗，是当今异位妊娠手术治疗最佳方法。

objective to evaluate the clinical value of early diagnosis and therapy of suspicious ectopic pregnancy by laparoscopy.methods the clinical data of 53 suspicious tubal pregnancy patients were analyzed retrospectively,who had received laparoscopic operation in our hospital from january 2003 to december 2005.results all cases received laparoscopic operation,none was transferred to traditional surgery during the operation.46 cases were diagnosed as tubal pregnancy during the operation with pathologic proof.preoperative diagnosis rate was 86.8%.the salpingecstomy were performed in the 32 cases.the salpingotomy were performed in the 7 cases.conclusion the laparoscopic operation is the best choice in the early diagnosis and therapy of suspicious ectopic pregnancy,and it gain time for conservative therapy.but not too early.

目的 评价可疑异位妊娠患者腹腔镜早期诊治的临床价值。方法收集2003年1月～2005年12月收治的经腹腔镜手术治疗的可疑输卵管妊娠患者53例资料进行回顾性分析。结果 53例手术均经腹腔镜完成，无1例中转，术中诊断输卵管妊娠的46例，术前诊断率86.8%，32例行输卵管切除术，7例行保守性手术。结论腹腔镜手术是早期确诊可疑异位妊娠患者的最佳选择，也为保守性治理赢得时间，但不宜操之过急。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。