腹水的

The uncovered tracts and the liver wound surface were managed by some of the methods combined such as high frequency electrocoagulation, titanium clamp, suture, hemostatic gauze oppression, argon-beam coagulation, protein glue adherence, and Endo-GIA switcher. The total operative outcome, operating time, blood loss, postoperative complications and postoperative hospital stay were summarized in order to assess feasibility and safety of the clinical laparoscopic liver resection.Results: 1. Laparoscopic liver resection with clamp dissection method needs only the ordinary laparoscopic equipment. It has a low cost but causes a little more bleeding. 2. Laparoscopic liver resection operated by microwave tissue coagulation method had very little bleeding when dissecting hepatic parenchyma. The operative field is clear and the cost is low. It is a safe, convenient and effective method. 3. Ultrasound dissector is a relatively ideal appliance for laparoscopic liver resection at present because ofless bleeding during operation. But the speed is slow thus prolongs the operation time. Its separating intensity is not strong enough, and it can only be applied to patients without hepatic cirrhosis. 4. Ligsure has clamp dissection and electrocoagulation functions. It can carbonize hepatic tissue including the tracts whose diameters are less than 7mm. It has some advantage in dissecting. However, it has the shortcomings of the clamp dissector during coagulating. 5. Hand-assisted laparoscopic liver resection can make use of the flexibility and feeling of the operators left hand.

结果：1、钳夹分离切肝法不需特殊仪器设备，只要具备常规的腹腔镜器械即可实施，成本低，肝断面较易渗血；2、微波固化切肝法肝实质出血很少，视野清晰，成本低，操作简单，是一种安全、简便、有效的方法；3、超声刀是目前较为理想的切肝器械，术中出血少，但是，切割速度慢，手术时间长，而且，切割强度有限，适用于无肝硬化的肝切除；4、Ligsure具有挤压粉碎及高频电凝止血两大功能，可使包括7~以下管道的肝组织碳化、形成焦痴，具有一定的优势，但是，也存在分离钳电凝止血的缺点；5、手辅助腹腔镜肝切除可以利用术者左手的灵活性及手感，便于术中显露、分离、控制出血等，可随意协助右手及助手的主要操作，缩短了手术时间，大大提高了腹腔镜肝切除的安全性，李朝龙等的改良方法，同样达到了手辅助目的，又节省了费用；6、小切口腹腔镜辅助切肝法可以使用剖腹肝切除常规器械及剖腹肝切除技术，操作简单、可靠，适用于肝左外叶切除及右肝第V段切除；7、Endo一GIA切肝法在离断肝组织的同时闭合管道结构，多用于肝左静脉、门静脉分支、管径较大胆管的切割，缺点是不能用于较厚肝组织的切割，而且价格昂贵；8、临床巧例腹腔镜肝切除均获得成功，手术时间最短1.5h，最长sh，平均125 min，多数在100 min左右，手术出血量最少50 ml，最多500 ml，平均1 78 ml，除2例合并严重肝硬化的原发性肝癌患者术后出现少量腹水，1例术后发生胆漏外，其余无并发症，发生胆漏的1例患者术后住院40d，多数在术后一周左右出院，术后住院时间5一40d，平均gd。

objective to study the anti-tumor activity and enhancing immune effect of phellinus linteus.methods the anti-tumor activity of phellinus linteus was estimated by the longevity of eac mice,the immune effect of tumorous mice was tested by the experiment of carbon particle expurgation and the il-2 concentration in serum of tumorous mice was determined by elisa.results phellinus linteus can prolong eac mouse's life and can increase the il-2 concentration in serum of tumorous mouse but can't affect the immune effect of tumorous mouse.conclusion chinese hurb phellinus linteus possess some antitumor power for tumor-loaded mice,but further research is needed.

目的 观察桑黄对肿瘤的抑制作用及对机体免疫功能的影响。方法以小鼠艾氏腹水癌的生命延长率评价桑黄的抗肿瘤作用，以炭末廓清实验观察荷瘤小鼠非特异性免疫功能，以elisa法测定荷瘤小鼠血清il-2浓度。结果桑黄对eac荷瘤小鼠的生存时间有延长作用，对荷瘤小鼠血清il-2浓度有提高作用，对荷瘤小鼠非特异性免疫功能无明显影响。结论中药桑黄对荷瘤小鼠具有一定的抗肿瘤能力，作用的强度和作用机制的深度还有待进一步研究。

We used Aeromonas hydrophila which we separated from the diseased Paralichthys olivaceus to made a inactivated whole cell bacterin .We injected this vaccine in the mice and the Paralichthys olivaceus .Both the mice and the Paralichthys olivaceus gained a good protection of 80% and 70%.The fish with dipping immersion gained a good protection of 85%,and the incidence of disease was 6%.We detected the antibody level in the antiserum by agglutination reaction and indirect ELISA which seemed to be immunogenic crucially.We anatomized the mice then we found that all the purtenance of the immunzied mice are healty.All of this proved that the vaccine is effective.

本实验将所分离到的嗜水气单胞菌用终浓度为0.2%~0.5%的甲醛对菌体进行灭活，制备了灭活疫苗，并优化灭活疫苗的灭活条件；将制备的灭活疫苗分别注射小鼠和牙鲆，均取得了较为理想的效果，其中小鼠的免疫保护率达到80%，牙鲆的免疫保护率达到70%；采用渗透压差法大规模浸泡免疫牙鲆也取得了很好的效果，浸泡免疫过的牙鲆发病率仅为6%，攻毒后免疫保护率为85%；通过应用间接ELISA方法检测受免动物的抗血清，并将牙鲆的抗血清注射新西兰白兔，从而获得兔抗鱼血清，更便于检测，结果都表明动物体内产生了较高的抗体水平；剖检攻毒后的动物，发现免疫过的动物内脏健康无异样，表明该疫苗安全有效，从而为防治牙鲆腹水症开辟了一条新途径，也为进一步制备其基因工程疫苗奠定了基础。

Results: Tyroserleutide can significantly increase the life span of H22 tumor-bearing mice by 50-70% in dosages of 20ug/kg/d-80ug/kg/d,specially the high dosage of 80ug/ml can significantly increase the life span by 69.24%; Tyroserleutide can inhibit the growth of transplanted hepatocellular tumor BEL-7402 in nude mice,the rate of tumor inhibition was25-50% in dosages of 40-320ug/ml ,the inhibition rate of 160ng/ml was 44.03%; Tyroserleutide could inhibit the growth of H22 and BEL-7402 tumor in a dose-dependent manner. Simultaneously, tumoricidal activity of tyroserleutide against BEL-7402 cell line in vitro was observed hinger when compared with the control group(P.05).The inhibition effect of 72hrs was higher than 24hrs,48hrs,96hrs.And specially the high dosage of 160ug/ml can significantly inhibit growth of tumor cell by 19.36%. Tyroserleutide can activated PEM and marked enhance cytotoxicity andphagocytosis functions in vitro and in vivo. The OD values of cytotoxicity were observed hinger when compared with the control group(P.05).The cytotoxicity of macrophages activated by tyroserleutide against BEL-7402 and B16-F10 was 35.58%,61.2% in vitro and21.39%,47.63% in vivo. The cytotoxicity rate of nude mice PEM was 32.86%,73.07% in vivo. Furthermore, tyroserleutide alone could stimulated the production of IL-1B TNF- a and NO by M . Tyroserleutide and LPS could synergistically activated M producing more cytotoxicity effectors. Conclusion: Tyroserleutide had inhibition functions against hepatoma carcinoma .Its possible mechanisms were related to the affect that Tyroserleutide could inhibit tumor cell directively and induce tumor cells apoptosis or death effectively.

结果：酪丝亮肽能显著延长腹水型肝癌H_(22)小鼠的生存时间，给药剂量为80μg／kg／d时疗效最显著，达到69.24％，在20μg／kg／d-80μg／kg／d剂量范围内生命延长率为50-70％，给药剂量与荷瘤鼠生存时间呈现一定量效关系；酪丝亮肽能显著抑制人肝癌BEL-7402移植瘤裸鼠的肿瘤生长，给药剂量为160μg／kg／d时疗效最显著，抑制率为44.03％，并且在40-320μg／kg／d剂量范围内抑制率为25-50％，给药剂量与肿瘤抑制率呈现一定量效关系；酪丝亮肽体外对人肝癌BEL-7402细胞生长有一定的抑制作用，在作用72hrs时各浓度酪丝亮肽对肿瘤细胞的抑制作用较24hrs、48hrs、96hrs明显，其中浓度为100μg／ml时抑制率达19.36％；酪丝亮肽体内外均能增强小鼠腹腔巨噬细胞对肿瘤细胞的杀伤：体外作用中巨噬细胞对BEL-7402、B16-F10的杀伤功能明显增强，与效应细胞对照组相比有显著性差异(P＜0.05)杀伤率分别达到35.58％、61.2％；体内作用中巨噬细胞对BEL-7402、B16-F10的杀伤功能明显增强，与生理盐水对照组相比有显著性差异(P 。05)，杀伤率分别达到21.39%、47.63%；裸鼠腹腔巨噬细胞经酪丝亮肤作用后对BEL一7402、B 16一F10杀伤功能明显增强，与生理盐水对照组相比有显著性差异(P.05)，最高杀伤率分别达到32.86%、73.07%；酪丝亮肤能增强单核巨噬细胞系统的吞噬功能，吞噬指数与生理盐水组比较有显著性差异(P.05)；酪丝亮肤体外作用能促进小鼠腹腔巨噬细胞分泌合成细胞毒效应分子IL一lp、TNF一Q和NO，与效应细胞对照组相比有显著性差异(P.05)；酪丝亮肤体内作用能促进小鼠腹腔巨噬细胞分泌合成细胞毒效应分子IL一lp、TNF一Q和NO，与生理盐水对照组相比有显著性差异(P.05)；酪丝亮肤能促进鼠巨噬细胞株R戌W264.7分泌合成IL一1p和NO，IL一1日、NO水平分别在酪丝亮肤作用24hrs、12hrs时达到高峰，酪丝亮肤单独应用能提高巨噬细胞的分泌合成功能，而且酪丝亮肤能与LPS协同作用刺激巨噬细胞的细胞毒效应分子分泌合成。

To determine the effects of ovarian steroid hormones and some cytokines on the expression of RANTES in cultured cells METHODS AND RESULTS The study included the following two parts 1 The Protein Production of RANTES in Peritoneal Fluids and the Expression of RANTES mRNA in Eutopic and Ectopic Endometrium of Patients with Endometriosis Twenty-eight patients with endometriosis were selected as study group and twenty patients without endometriosis and maglinant disease were selected as control group . In our study, 10 samples of peritoneal fluid in Em and 10 samples of in En were taken, 13 specimens of ovarian endometrioma , 3 red peritoneal endometriotic lesions , 2 uterosacral ligament nodules , 17 matched eutopic endometrium and 12 specimens of endometrium in En were biopsied.

研究目的 1 从蛋白水平检测RANTES在子宫内膜异位症患者腹水中的表达，从核酸水平比较RANTES在内异症患者的异位病灶和在位内膜与非内异症患者子宫内膜之间表达的差异，探讨RANTES在内异症患者盆腔炎性反应中的作用；2 体外培养在位和异位的子宫内膜细胞，研究细胞因子TNFα、INFγ、雌孕激素以及PPARγ激动剂15-d-PGJ〓对RANTES表达的影响，探讨这些因素在内异症发病机理及内异症患者盆腔炎性反应中的作用。

Therefore, the author excogitates some synthesis courses of new compounds including selenium and aspirin based on medicament chemistry and coalition principle. The new compound is named 5-5'-diselenium monoacetyl salicylic acid and ascertained by IR ,UV and MALDI-TOF-MS, and its structure is: OOHThe compound is inhibiting to sarcoma and ascites tumour of white mice by pharmacology experiments, so it is worth researching deeply.

为此，本文运用化学拼合原理，提出了几种既含硒又含乙酰水杨酸的化合物新结构的合成路线，经过多次理论分析和探索试验，得到一种预测的新的化合物，命名为5-5′-二硒单乙酰水杨酸，经红外、紫外、飞行时间质谱测定，证明该化合物的结构为：经药理预试验表明，该化合物对小鼠S_(180)肉瘤和艾氏腹水型肿瘤均有抑制作用，具有深入研究的意义。

This study suggests that some unusual sonographic findings, especially an area of ascites with incomplete mobile septations or particulate ascites, should be considered indicative of tuberculous peritonitis.

本研究认为当出现一些不常见的超声表现，尤其是腹水内出现不完全的可活动的分隔或光点回声时应该考虑到结核性腹膜炎的可能。

This study suggests that some unusual sonographic findings, especially an area of ascites with incomplete mobile septations or particulate ascites, should be considered indicatie of tuberculous peritonitis.

本研究认为当出现一些不常见的超声表现，尤其是腹水内出现不完全的可活动的分隔或光点回声时应该考虑到结核性腹膜炎的可能。

CPLP sensitized with bovine serum albumin were chequerboard titrated by antibody against BSA, and effects of sensitized conditions on latex agglutination test were studied.

结果表明，以0.05 mol／L，pH8.0的Na_2HPO_4-NaH_2PO_4缓冲液稀释CPLP乳液至1.0％，BSA的终浓度为0.5 mg／mL，37℃致敏30 min时，与1∶160稀释的腹水抗体(效价1∶40，000)发生凝集反应的效果最好。

Recoveries between 95 %～108 % have been achieved in the application of the method to measurements of Al in biological samples such as synthetic renal dialysate, Ringer's solution, human blood, cerebrospinal fluid of patients, and urine of diabetic patients.

采用标准加入法将所建立的方法应用于实际样品如人全血、脑病患者的脑脊液、糖尿病患者的尿、肺癌患者的腹水、合成肾透析液及合成脑脊液等样品中铝测定，结果表明回收率在95%～108%之间，所测得的样品中铝含量与文献值和ICP法值吻合。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。