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The titres of ascitic fluids of four MAbs ranged from 1:80000 to 1:5120000 when tested by indirect ELISA.

各株单抗腹水的ELISA效价均在1:80000-1:5120000之间。

Objective To observe the effect of curcumin on biological behavior such as proliferation and metastatic of ascitic hepatocarcinoma cell strain with high metastatic potential in lymphatic system of mice.

目的 研究姜黄索对小鼠腹水型肝癌高淋巴道转移细胞株生长和转移行为的影响。

Culture supernatant and ascitic titres of the antibodies were 1 ∶ 500,1 ∶ 8.0×104,when tested by ELISA respectively.

用间接ELISA法检测细胞上清单抗效价为1∶ 500;单抗腹水滴度为1∶ 8.0× 104。

Ascitic fluid should be inoculated into blood culture bottles at the bedside.

腹水应该在床旁接种入血培养瓶。

Purpose: To evaluate the diagnostic value of the combined assay of tumor mark in seurm and ascitic fluid to the alimentary system malignant neoplasm.

目的:探讨联合测定血清与腹水肿瘤标志物对消化系恶性肿瘤的诊断价值。

Ascitic fluid should be inoculated into blood culture bottles at the bedside.

腹水应该在床边接种入血培养瓶中。

The titre of ascitic fluids of MAb against the coat protein of RBSDV was 1:1280 in ACP-ELISA.

该单抗腹水对RBSDV病叶的ACP-ELISA检测灵敏度为1:1280。

The McAb titers of three hybridoma cells lines from ascetic fluid were 1∶6000, 1∶6000 and 1∶12000 by ELISA test respectively. The sensitivity of the McAbs was 102-103 cfu. The McAbs did not have cross-reactivity with any other bacteroides and clostridia.

结果:建立了3株稳定的杂交瘤细胞株,ELISA法测定腹水McAb效价分别为1∶6000,1∶6000和1∶12000,敏感性为102-103 cfu,与其它杆菌、梭菌无交叉反应,竞争抑制试验表明3株McAb作用于细胞表面不同的抗原决定簇。

Preliminary bioassay: Compounds I, VI, VII, XXIII and XIVshowmoderate cytotoxicities against Ehrlich tumor cells (〓 30-40μ g/ml), while compound 1 exhibites stronger activity (〓 10.5μ g/ml). Saturated butanolide was almost inactive.

化合物Ⅰ、Ⅵ、Ⅶ、ⅪⅤ和XXⅢ的初步生理活性试验表明,它们均对艾氏腹水瘤细胞表现有中等的细胞毒性,其〓大多在30~45微克/毫升之间,含有双键的不饱和内酯Ⅰ和XIV有较好的活性,其中尤以Ⅰ的活性最强,〓为10.5微克/毫升。

For purification of McAb,the results suggested that ion exchange chromatography method can be applied for analysis and caprylic acid method for a very large volume of ascitic fluid.

单抗纯化结果显示离子交换层析法适用于科研分析,而辛酸沉淀法则适用于大量腹水中单抗的纯化。

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关闭眼睛,深呼吸,一切不再是梦想,犹如。。。。。。