腹水

In patients with ascitic fluid neutrophil count of ＞250 cells/mm3, empiric antibiotic therapy should be started.

治疗 腹水嗜中性粒细胞计数＞250细胞/mm3的患者，应该开始进行经验性的抗生素治疗。

Results : The purity of the antibody was estimated to be greater than 90% after euglobulin precipitation combined with gel filtration, but less than 60% after ammonium sulfate precipitation combined with gel filtration.

腹水含有大量的杂蛋白，采用一步纯化法，不可能得到高纯度的IgM抗体，而多步骤纯化因耗时长，抗体活性损失大。

Euglobulin precipitation combined with gel filtration is a good method for purification IgM Mabs from ascites.

本文通过优球蛋白沉淀结合凝胶过滤法纯化腹水IgM型抗体，并与传统的硫酸铵沉淀结合凝胶过滤法相比较。

Methods:Ascites fluid was subjected to ammonium sulfate precipitation or euglobulin precipitation.

分别以硫酸铵沉淀或优球蛋白沉淀结合凝胶过滤法，从腹水中纯化IgM型单克隆抗体。

Application of McAbs: the ascites of McAb A_12B_4 were purified with euglobulin precipitation, and tagged with colloidal golds, then the immuno-gold filtration assay was developed to examine avian influenza virus. The results showed that the positive outcome was gained with avian H_9, H_7 and H_5 influenza virus.

单抗的初步应用：A1284株腹水经优球蛋白沉淀法纯化后与胶体金标记，应用于胶体金试纸条检测试验，金标A1284株单抗与H9、H7、H5三种亚型禽流感病毒反应结果呈阳性。

Methods anti-tumor activity of Phellinus linteus was estimated by the longevity of EAC mice,the immune effect of tumorous mice was tested by the experiment of carbon particle expurgation and the IL-2 concentration in serum of tumorous mice was determined by ELISA.

以小鼠艾氏腹水癌的生命延长率评价桑黄的抗肿瘤作用，以炭末廓清实验观察荷瘤小鼠非特异性免疫功能，以ELISA法测定荷瘤小鼠血清IL-2浓度。

Methods The anti-tumor activity of Phellinus linteus was estimated by the longevity of EAC mice,the immune effect of tumorous mice was tested by the experiment of carbon particle expurgation and the IL-2 concentration in serum of tumorous mice was determined by ELISA.

目的 观察桑黄对肿瘤的抑制作用及对机体免疫功能的影响方法以小鼠艾氏腹水癌的生命延长率评价桑黄的抗肿瘤作用，以炭末廓清实验观察荷瘤小鼠非特异性免疫功能，以ELISA法测定荷瘤小鼠血清IL-2浓度。

Methods The anti-tumor activity ofPhellinus linteus was estimated by the longevity of EAC mice,theimmune effect of tumorous mice was tested by the experiment ofcarbon particle expurgation and the IL-2 concentration in serum oftumorous mice was determined by ELISA.

以小鼠艾氏腹水癌的生命延长率评价桑黄的抗肿瘤作用，以炭末廓清实验观察荷瘤小鼠非特异性免疫功能，以ELISA法测定荷瘤小鼠血清IL-2浓度。

objective to study the anti-tumor activity and enhancing immune effect of phellinus linteus.methods the anti-tumor activity of phellinus linteus was estimated by the longevity of eac mice,the immune effect of tumorous mice was tested by the experiment of carbon particle expurgation and the il-2 concentration in serum of tumorous mice was determined by elisa.results phellinus linteus can prolong eac mouse's life and can increase the il-2 concentration in serum of tumorous mouse but can't affect the immune effect of tumorous mouse.conclusion chinese hurb phellinus linteus possess some antitumor power for tumor-loaded mice,but further research is needed.

目的 观察桑黄对肿瘤的抑制作用及对机体免疫功能的影响。方法以小鼠艾氏腹水癌的生命延长率评价桑黄的抗肿瘤作用，以炭末廓清实验观察荷瘤小鼠非特异性免疫功能，以elisa法测定荷瘤小鼠血清il-2浓度。结果桑黄对eac荷瘤小鼠的生存时间有延长作用，对荷瘤小鼠血清il-2浓度有提高作用，对荷瘤小鼠非特异性免疫功能无明显影响。结论中药桑黄对荷瘤小鼠具有一定的抗肿瘤能力，作用的强度和作用机制的深度还有待进一步研究。

In vivo tumor metastasis assay, three group cells were inoculated into footpad of 615 mouse subcutaneously. After three weeks, the mice were killed and their lymph nodes were isolated.

结果：1.Gelsolin在小鼠腹水型肝癌细胞系Hca-F细胞和Hca-P细胞胞浆中表达，Western-blot分析显示gelsolin在Hca-F细胞中高表达，在Hca-P细胞中表达较低(P.05)。2。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。