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腓肠肌

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The crutching and walking function of the leg reconstructed at last Conclusion When all the necrotic intermuscular vessels and muscles except caput mediale of gastrocnemius were removed, the necmsis may be delayed by means of reticular collateral circulation among skin, deep and superficial fascia and tibia of the injured limb. However, the blood supply is very little, the severe ischemia wound and ischemia pain can't be healed.

腓肠肌内侧头以外全小腿肌肉、肌间血管均坏死被切除时,小腿皮肤、深浅筋膜组织、腓肠肌内侧头肌瓣与胫骨内血供系统在踝以下所构成的网状侧支循环能为患足提供微量血供,延缓足的坏死,但仅凭这一微量血供无法治愈广泛贫血创面和顽固性肢体缺血痛。

Method]reversed sural neurofasciocutaneous flap with muscle was applied for repairing the soft tissue defect simultaneous open fracture of tibia lower section 6 cases,the soft tissue defect simultaneous chronic osteomyelitis of tibia lower section 5 case,the soft tissue defect of sole of foot simultaneous calcaneus epicarp 3 case, the soft tissue defect of sole of foot simultaneous calcaneus osteomyelitis or dead space 2 case. the scope of the flap was 18 c m×13 cm~11 c m×7 c m and that of the gastrocnemius flap was 4 cm×3 cm~9 c m×6 cm,the thickness was 1~3 cm,the skin flap was bigger than the muscle flap.

方法]自2004年6月以来,应用带肌肉的腓动脉穿支蒂腓肠神经营养血管皮瓣修复小腿下段及足踝部软组织缺损16例,其中胫骨下段开放性骨折伴软组织缺损6例,胫骨下段软组织缺损伴慢性骨髓炎5例,足后跟足底软组织缺损伴跟骨表层组织缺损3例,跟骨骨髓炎伴窦道2例,皮瓣面积30 cm×10 cm~6 cm× 4.5cm,切取的腓肠肌瓣面积4 cm×3 cm~9 cm×6 cm,肌肉厚度1~3 cm,皮瓣面积比肌瓣的面积要大。

Methods 26 patients with gastrocnemius spasticity after stroke were randomly divided into ultrasonic group and control group. The stretch therapy was used in the two groups, and the continuous ultrasonic wave therapy was added on ultrasonic group for 4 weeks. The scores of modefied Ashworth scale, the ratio of Hmax and Mmax, and the fascicle length of gastrocnemius pre and post treatment were observed and compared respectively in the two groups.

26例脑卒中后腓肠肌痉挛的患者随机分为超声波组和对照组,两组在给予腓肠肌牵张治疗的基础上,超声波组采用超声治疗仪对患侧腓肠肌进行连续超声波治疗4周;比较治疗前后两组改良的Ashworth量表评分、胫神经H反射的最大H波和M波比值、腓肠肌肌束长度。

Anatomical study proved:①.4~8 nutritional vessels of sural nerve –small saphenous vein were the intermuscular perforators of peroneal artery, the furthermost one located at(1.0±1.3)cm above the tip of external malleolus with diameter (0.6±0.2)mm;②.2~6 musculocutaneous perforators were given off by the artery of outer head of gastrocnemius muscle to supply nutrition to the sural nerve-small saphenous vein;③.intermuscular perforators from peroneal artery supplied blood to fibula and outer head of soleus muscle.the perforators with an average outer diameter of (1.3-1.6)cm gave off cutaneous branch ,fascial branch and nutritional vessels to the sural nerve-small saphenous vein,which formed the vessel chain of the sural nerve-small saphenous vein ,sub-fascial and suprafascial vessel net.

结果:1、解剖实验证实:①、腓肠神经-小隐静脉营养血管来自腓动脉肌间隔穿支4~8支,最远的跟外侧动脉穿支距外踝尖上(1.0±1.3)cm,外径(0.6±0.2)mm。②、腓肠肌外侧头动脉沿途发2-6支的肌皮穿支营养腓肠神经-小隐静脉。③、腓动脉肌间隔穿支营养腓骨、比目鱼肌外侧半。上述穿皮支平均外径在0.3~1.6mm,发出皮支、筋膜支、腓肠神经-小隐静脉营养血管,形成腓肠神经-小隐静脉血管链以及深、浅筋膜血管网。

130 rats were randomly divided into five groups: 5F electroacupuncture treatment group, 50Hz electroacupuncture treatment group,100Hz electroacupuncture treatment group, the model group and the control group. 4mm defect was produced in the left sciatic nerve of each rat and silicon nerve guards were used to bridge the nerve defect not including the rats of the control group. After 10 weeks electroacupuncture treatment, gross observation, histological examination of the sciatic nerve were carried with method of HE stainning and S-100 immunhistochemical staining, Sevier-Munger stainnning. The histomorphometric examination of the triceps muscles were observed with measuring width muscle cell and the wet weight, and measuring the content of the heparin and the lactic aid of the triceps muscle with biochemistry methods.

实验方法:将130只Wistar大鼠随机分为五组:正常组、造模组、电针5Hz组、电针50Hz组、电针100Hz组,除正常组外,其余各组手术切取坐骨神经4mm后,做坐骨神经硅胶管桥接术,造成周围神经损伤模型,取患侧足三里、三阴交、环跳穴,术后第四天开始电针,每两天一次,每次10分钟,治疗10周后处死动物,取出硅胶管内新生的坐骨神经,纵切片,HE染色、S100免疫组化染色、Sevier-Munger银染,观察坐骨神经纤维再生情况,以健侧同段坐骨神经做对照;取患侧脖肠肌称取湿重,HE染色,观察腓肠肌肌细胞直径,生化测量腓肠肌肌糖元、乳酸。

ETHODS: The gastrocnemius bundle branch and soleus muscle bundle branch of the right achilles tendon were sutured after surface skin and subcutaneous tissue discission. The gastrocnemius bundle branch was transected at 2 cm above calcaneal bone insertion and a 2-cm silicone tube was applied to separate the site of suture using 5-0 modified Kessler method. The site of suture was treated with nothing in silicone tube as the blank control group. In other three groups, the site of suture was treated with 100, 50, 10 ng VEGF in silicone tube at the operation day and 7, 14, 21 days post-operation.

切开家兔右侧跟腱表面皮肤、皮下组织,钝性分离跟腱的腓肠肌束支和比目鱼肌束支,于跟骨止点上方约2 cm处横行切断跟腱腓肠肌束支,将2 cm长硅胶管套入一侧断端,5-0缝合线改良Kessler法缝合跟腱,缝合后将硅胶管覆盖跟腱缝合处,空白组硅胶管内不加药,其他3组于手术当日及术后第7,14,21天往硅胶管内加入血管内皮生长因子100,50,10 ng。

objective to study the effects of ethanol extract of the huangqi on the skeletal muscles contractility in rats.methods the gastrocnemius and soleus muscle contractility were observed by stimulating the sciatic nerve with electricity after affusing stomach with ethanol extract of the200mg/kg dosage of huangqi for2weeks.results the ethanol extract of the huangqi could shorten latent period,increase maximal contractive extent and time and relaxative time at the twitch and complete tetanus of gastrocnemius and soleus muscles.conclusion the ethanol extract of the huangqi can enhance skeletal muscles contractility in rats.

方法] 灌胃给予200mg/kg黄芪酒精提取液2周后,观察大鼠坐骨神经受刺激后腓肠肌及比目鱼肌的收缩力。[结果]黄芪酒精提取液缩短腓肠肌及比目鱼肌单收缩和完全性强直收缩潜伏期,增加单收缩最大收缩幅度,延长最大收缩时间及最大舒张时间;增加完全性强直收缩的最大收缩度,延长最大收缩时间。[结论]黄芪酒精提取液可增强大鼠骨骼肌的收缩力。

Methods BTXA labeled with125Iwas obtained. The radioactivitymeasurementwas taken tomeasure the solubility ofBTXA in gel and BTXA solution. The rightgastrocnemiusmuscles of12 SD ratswere injectedwithBTXA geland the leftgastrocnemiusmuscles injectedwith normal salinewere used as controls. HE staining and 1% gold chloride stainingwere used to study the tissue changes 6 months and 12 months afterBTXA gel injection and transmission electronmicroscopy was used to observe the ultrastructural changes.

将BTXA行示踪标记配制125I-BTXA水凝胶与125I-BTXA水溶液,进行样品放射性强度测定。12只SD大鼠用随机数字表法分为A、B两组,每组6只,大鼠右侧腓肠肌注射BTXA水凝胶,左侧腓肠肌注射等体积生理盐水作对照,于注射后6个月、12个月切取腓肠肌标本,常规病理HE染色、1%氯化金染色,透射电子显微镜观察组织结构变化。

Thirty SD rats were randomly divided into five groups: control, 1 week, 2 week, 3 week and 4 week and stimulating the right triceps surae was stimulated by electrical stimulator. Using real-time quantitative PCR measurement to measure two alternative splices of IGF-1(IGF-1Ea and MGF) and MyoG mRNA expressing of gastrocnemius and right biceps brachii.

SD大鼠30只,随机分为5组,每组6只,分别为对照组和1、2、3、4周电刺激组,刺激部位为右侧腓肠肌,实时荧光定量PCR法分别检测双下肢腓肠肌和前肢肱二头肌的胰岛素样生长因子-1(IGF-1)不同变构体肝型胰岛素样生长因子(IGF-1Ea)和机械力生长因子以及成肌分化因子(Myogenin, MyoG)mRNA表达。

The treadmill training of T-group rats were continuously trained for 4~6 weeks at the intensity of about 75% VO2max (18??5~24m/min,gradient of 0°,each motion lasting 50 minutes,twice a day). Detect the content of gastrocnemius MHC mRNA by gastrocnemius reverse transcription polymerase chain reaction,and detect the changes of MHC muscle fiber and its size of cross section area through immunohistochemistry. Meanwhile,apply the electric stimulation to determine the maximal tension of isometric contraction of post-training gastrocnemius.

运动训练组大鼠进行连续4~6wk强度约为75%VO2max(18.5~24m/min,坡度为0°,每次运动50min,每天2次)的跑台训练,反转录聚合酶链式反应腓肠肌MHC mRNA含量,以免疫组化方法检测肌球蛋白肌纤维的改变情况及横截面积的大小,同时运用电刺激观察训练后腓肠肌等长收缩最大张力。

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